164,765 results

1. GFP-Nucleolin

   Plasmid

   #28176

   Depositor

   Michael Kastan

   Article

   Takagi et al Cell. 2005 Oct 7. 123(1):49-63.

   Insert

   Nucleolin (NCL Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-GFP11(x7)-Actin

   Plasmid

   #181967

   Purpose

   Human beta actin tagged with 7 copies of GFP11.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   GFP11(x7)-Actin (ACTB )
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV mClover3-Galectin-3

   Plasmid

   #215376

   Purpose

   Expression of the endolysosomal damage marker Galectin-3 with an N-terminal mClover3 tag.

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   LGALS3 (LGALS3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV mRuby3-Galectin-3

   Plasmid

   #215375

   Purpose

   Expression of the endolysosomal damage marker Galectin-3 with an N-terminal mRuby3 tag.

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   LGALS3 (LGALS3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus

   Plasmid

   #61629

   Purpose

   R2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoter

   Depositor

   Dolf Weijers

   Article

   Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

   Inserts

   mDII
   ntdTomato
   DII
   n3xVenus
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   RPS5A

   Available Since

   March 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC

   Plasmid

   #183790

   Purpose

   A single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.

   Depositor

   Jay Shendure

   Article

   Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

   Inserts

   P2A-eGFP-TargetBC-5xTAPE-1
   pegRNA-InsertBC
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   G>A in the gRNA scaffold- please see depositor…

   Promoter

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD24-sfGFPx1

   Plasmid

   #51558

   Purpose

   Superfolder GFP ORF cloned into pBAD24 for expression in E. coli

   Depositor

   Francisco Malagon, Sankar Adhya

   Article

   Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

   Insert

   superfolder GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHREAC

   Plasmid

   #134908

   Purpose

   Overexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Download fully annotated map under Resource Information.

   Depositor

   George Lomonossoff

   Article

   Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   double 35S

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. VAMP3-mCherry

   Plasmid

   #92423

   Purpose

   Expression of VAMP3 C-terminally conjugated to mCherry.

   Depositor

   Geert van den Bogaart

   Article

   Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

   Insert

   VAMP3 (Vamp3 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME-Cas9-T2A-GFP

    Plasmid

    #63155

    Purpose

    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Insert

    Cas9-T2A-GFP
    

    Use

    CRISPR; Tol2 middle entry vector for gateway

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-N-WASP

    Plasmid

    #47406

    Depositor

    Peter McPherson

    Article

    Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

    Insert

    N-WASP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-SpdCas9-VP64

    Plasmid

    #115794

    Purpose

    Expresses SpdCas9-Vp64 fusion protein when packaged into AAV

    Depositor

    Nadav Ahituv

    Article

    Matharu et al Science. 2018 Dec 13. pii: science.aau06

    Insert

    VP64
    

    Use

    AAV

    Tags

    3XFLAG and dCas9-VP64

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-CMV-Luc2-IRES-ZsGreen-W

    Plasmid

    #164432

    Purpose

    Lentiviral vector backbone encoding firefly luciferase and ZsGreen

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Inserts

    Luciferase
    ZsGreen
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. EFSp-GFP-YAP

    Plasmid

    #174168

    Purpose

    Lentiviral vector to constitutively express YAP under control of the EFS promoter.

    Depositor

    Rod Bremner

    Article

    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

    Insert

    YAP (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    3x FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    Aug. 19, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. DLX2-hygro

    Plasmid

    #97330

    Purpose

    Generating lentiviruses to express Dlx2 and hygromycin resistance

    Depositor

    Marius Wernig

    Article

    Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

    Insert

    DLX2 (Dlx2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TetO

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS-Hsp70-Cas9

    Plasmid

    #46294

    Purpose

    A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.

    Depositor

    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

    Article

    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

    Insert

    codon optimized Cas9
    

    Use

    CRISPR

    Tags

    3x Flag

    Expression

    Insect

    Mutation

    Silent change from C to A at bp 834 of insert

    Promoter

    Hsp70

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. hGli3 flag3x

    Plasmid

    #84921

    Purpose

    mammalian expression of Gli3

    Depositor

    Martin Fernandez-Zapico

    Insert

    Gli3 (GLI3 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. CaV2.1/pcDNA3.1 ~ Human

    Plasmid

    #140575

    Purpose

    Expression in mammallian cells or oocytes

    Depositor

    Dan Minor

    Article

    Abderemane-Ali et al Neuron. 2019 Mar 20;101(6):1134-1149.e3.

    Insert

    CaV2.1 Human (CACNA1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBad-sfGFP 150TAG

    Plasmid

    #85483

    Purpose

    Arabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150

    Depositor

    Ryan Mehl

    Article

    Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

    Insert

    superfolder GFP 150TAG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    150TAG (amino acid 150 mutated to stop codon)

    Promoter

    Arabinose

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDD282

    Plasmid

    #66823

    Purpose

    GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    GFP-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized GFP

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGP-AAV-syn-iGABASnFR2n-WPRE

    Plasmid

    #218875

    Purpose

    AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2n
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S99A F104H R168P

    Promoter

    Synapsin

    Available Since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pBPLexA::p65Uw

    Plasmid

    #26231

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    LexA::p65 (RELA Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE-AXL

    Plasmid

    #116714

    Purpose

    Lentiviral expression of AXL

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    AXL (AXL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-PE6g

    Plasmid

    #207857

    Purpose

    Mammalian expression of PE6g prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6g
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Cas9(H840A, E471K, H99R, I632V, H721Y, R654C, D64…

    Promoter

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PPRE-H2B-eGFP

    Plasmid

    #84393

    Purpose

    Fluorescent reporter for PPARgamma activity

    Depositor

    Severine Degrelle, Thierry Fournier

    Article

    Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

    Insert

    PPARG response element (PPARG Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    PPRE

    Available Since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. FLHKI-pGFPN3

    Plasmid

    #21917

    Depositor

    Hossein Ardehali

    Article

    Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

    Insert

    Full length human HKI in pGFP-N3 (HK1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    This is full length human HKI in pGFP-N3

    Promoter

    Available Since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-CaMKII(0.3)-mVenus-FKBP-K7GEF

    Plasmid

    #225828

    Purpose

    A component of SYNCit-K (Synapse-targeted chemically induced translocation of Kalirin-7)

    Depositor

    Haruo Kasai

    Article

    Sawada et al Science. 2024 Sep 27;385(6716):1459-1465

    Insert

    mVenus-FKBP-K7GEF
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pICSL50009

    Plasmid

    #50309

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    C terminal HA tag (6x Human influenza hemagglutinin)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PB-U6insert-EF1puro

    Plasmid

    #104537

    Purpose

    A piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAs

    Depositor

    George Church

    Article

    Kalhor et al Science. 2018 Aug 9. pii: science.aat980

    Type

    Empty backbone

    Use

    CRISPR, Mouse Targeting, and Synthetic Biology ; …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. p3E_he1a:mCherry

    Plasmid

    #113881

    Purpose

    p3E entry vector for Three Multisite Gateway

    Depositor

    Marnie Halpern

    Insert

    he1a (he1.1 Zebrafish)
    

    Use

    Zebrafish danio expression

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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