Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 13,263 results for: cDNA

Showing: 7081 - 7110 of 13263 results

  2. pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations (NT51)

    Plasmid
    #49061
    Purpose
    Expresses human NKCC1 mutated Ser and Thr residues in the N-terminus and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    S150N, S155Q, S170Q, T177A, S183R, S193E, T203A, …
    Promoter
    CMV
    Available Since
    Nov. 19, 2013
    Availability
    Academic Institutions and Nonprofits only
    49061-plasmid-map-sequence-id-72183

    Enlarge

  4. pcDNA4/TO-myc NES-mCE(K294A) NLS-Flag

    Plasmid
    #82469
    Purpose
    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme, inactive form
    Depositor
    Daniel Schoenberg
    Article
    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d
    Insert
    mRNA capping enzyme (Rngtt Mouse)
    Use
    Tags
    Flag and Myc
    Expression
    Mammalian
    Mutation
    changed Lysine 294 to Alanine
    Promoter
    CMV
    Available Since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    82469-plasmid-map-sequence-id-163073

    Enlarge

  9. pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKL

    Plasmid
    #73210
    Purpose
    Expresses myc-dL5(E52D)-mCer3 fusion protein in peroxisomes of mammalian cells with a C-terminal PTS (SKL). (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Insert
    KozATG-myc-dL5-2XG4S-mCer3-SKL (MYC Synthetic, Human)
    Use
    Tags
    The FAP and mCerulean3 are fused with 2 copies of…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available Since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73210-plasmid-map-sequence-id-139746

    Enlarge

  10. pcDNA3.1 Flag YFP hNKCC1 extracellular-cysteineless (NT864)

    Plasmid
    #49069
    Purpose
    Expresses human NKCC1 mutant lacking extracellular cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C563S,C568S,C577S,C582S in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    49069-plasmid-map-sequence-id-72196

    Enlarge

  17. pcDNA4TO T21R-F8.2 (S54L+T127A)-T2A-mCherry

    Plasmid
    #225584
    Purpose
    Mammalian expression vector for Doxycycline inducible expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter
    Depositor
    William McEwan
    Article
    Benn et al Science. 2024 Aug 30;385(6712):1009-1016
    Insert
    T21R-F8.2 (S54L+T127A)-T2A-mCherry
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    T21R-F8.2 (S54L+T127A)
    Promoter
    CMV
    Available Since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    225584-plasmid-map-sequence-id-446523

    Enlarge

  20. pCDNA3.1(+)-hSyn-HA-mTREK-1(S131C/A286F)-IRES-GFP

    Plasmid
    #225490
    Purpose
    Neuronal expression IRES-containing bicistronic vector for generating mTREK-1 (S131C/A286F) + EGFP under the hSyn promoter.
    Depositor
    Dan Minor
    Article
    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1
    Insert
    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    Use
    Neuronal expression
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available Since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    225490-plasmid-map-sequence-id-448327

    Enlarge

  21. pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2-GABARAP(G116A)

    Plasmid
    #212108
    Purpose
    Expression of lipidation deficient GABARAP split tandem protein with mTagBFP2
    Depositor
    Dieter Willbold
    Article
    Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.
    Insert
    GABARAP(G116A)-mTagBFP2-GABARAP(G116A) (GABARAP Human, Entacmaea quadricolor)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    G116A (lipidation deficiency) of both GABARAPs
    Promoter
    CMV
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    212108-plasmid-map-sequence-id-420140

    Enlarge

Showing: 7081 - 7110 of 13263 results