We narrowed to 14,145 results for: TIM
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Plasmid#213351PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only
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PK1-DuET
Plasmid#213366PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PRRP-DuET
Plasmid#213367PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PTAFR-DuET
Plasmid#213368PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
QRFP-DuET
Plasmid#213372PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
P2RY8-DuET
Plasmid#213365PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
NPS-DuET
Plasmid#213353PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
MRGPRG-DuET
Plasmid#213343PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSTR5-DuET
Plasmid#213380PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
SUCNR1-DuET
Plasmid#213381PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
S1PR4-DuET
Plasmid#213375PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSTR1-DuET
Plasmid#213376PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PTGER1-DuET
Plasmid#213370PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrA_F164R238
Plasmid#216230PurposeS. aureus USA300 GyrA mutant F164 R238 residues codon-optimized for E. coliDepositorInsertDNA gyrase
Tags6xHisExpressionBacterialMutationChanged arginine 238 to serine, phenylalanine 168…PromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB_A467
Plasmid#216234PurposeS. aureus USA300 GyrB mutant A467 residue codon-optimized for E. coliDepositorInsertDNA gyrase
Tags6xHisExpressionBacterialMutationChanged alanine 467 to valinePromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB_T522
Plasmid#216235PurposeS. aureus USA300 GyrB mutant T522 residue codon-optimized for E. coliDepositorInsertDNA gyrase
Tags6xHisExpressionBacterialMutationChanged threonine 522 to isoleucinePromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP4-EGFP
Plasmid#210378PurposeExpresses SARS-CoV-2 NSP4 fused with EGFP in mammalian cellsDepositorInsertNSP4 (ORF1ab SARS-CoV-2)
TagsEGFPExpressionMammalianMutationMany synonymous changes due to codon optimizationAvailable SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP2-Flag
Plasmid#210344PurposeExpresses SARS-CoV-2 NSP2 in mammalian cellsDepositorInsertSARS-CoV-2 NSP2 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
TagsFlagExpressionMammalianMutationMany synonymous changes due to codon optimizationAvailable SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET23a-H6-TEV-AncSZ
Plasmid#214235PurposeBacterial expression construct of AncSZ, an kinase domain engineered through ancestorial reconstruction of the kinase domains of both SYK and ZAP-70. For in vitro protein tyrosine phosphorylationDepositorInsertSyk kinase (SYK Human)
Tags6xHisExpressionBacterialMutationSequence gained through ancestral reconstruction …PromoterT7Available SinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
LLP782_Traptavidin-KRAB-CO
Plasmid#211775PurposeAviTag counterpart binding domain, Traptavidin, fused to transcriptional repressor KRAB, with GFP selectionDepositorInsertSpyCatcher-DNMT3A
Tags3xFLAGExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available SinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP3C-mCherry
Plasmid#210377PurposeExpresses the C terminal of SARS-CoV-2 NSP3 fused with mCherry tag in mammalian cellsDepositorInsertNSP3C (ORF1ab SARS-CoV-2)
TagsmCherryExpressionMammalianMutationMany synonymous changes due to codon optimizationAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
LLP785_Traptavidin-DNMT3A-CO
Plasmid#211778PurposeAviTag counterpart binding domain, Traptavidin, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertTraptavidin-EZH2
Tags3xV5ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect EZH2 e…PromoterpEF1a and pSV40Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP781_SnoopCatcher-KRAB-CO
Plasmid#211774PurposeSnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional repressor KRAB, with GFP selectionDepositorInsertTraptavidin-KRAB
Tags3xV5ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect KRAB e…PromoterpEF1a and pSV40Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
(B) NAT marker (R) (SBE173)
Plasmid#195014Purposenourseothricin (NAT) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOSDepositorInsertpXYL+NAT+tNOS
ExpressionBacterialPromoterpXYLAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(B) G418 marker (R) (SBE 137)
Plasmid#195011Purposegeneticin (G418) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOSDepositorInsertpXYL+G418+tNOS
ExpressionBacterialPromoterpXYLAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
p202-Syt1-C2A-wt
Plasmid#201553PurposeExpresses wild-type Octopus synaptotagmin-1 C2A domain in E. coliDepositorInsertSynaptotagmin-1 C2A domain (partial)
ExpressionBacterialMutationCodon-optimized for E. coliPromoterRBSAvailable SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynW pHluorin-SYT9 IRES mRuby3
Plasmid#195698PurposeLentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoterDepositorAvailable SinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynW Syt9 IRES mRuby3
Plasmid#195700PurposeLentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoterDepositorAvailable SinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TEV-A7 (2-180)
Plasmid#190066PurposeCodon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain.DepositorInsertAnnexin A7 PRD (ANXA7 Human)
TagsGB1-His-TEVExpressionBacterialMutationPRD only, residues 2-180Available SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TEV-A7-Strep (2-180)
Plasmid#190067PurposeCodon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain with strep.DepositorInsertAnnexin A7 PRD (ANXA7 Human)
TagsGB1-His-TEV and StrepExpressionBacterialMutationPRD only, residues 2-180Available SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 4 CMV eGFP
Plasmid#206272PurposeENTR Vector 4 for MultiSite Gateway assembly. Encodes eGFP under the control of a CMV promoterDepositorInserteGFP
UseMultimate/gateway entr 4ExpressionMammalianAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 4 CMV mTagBFP
Plasmid#206279PurposeENTR Vector 4 for MultiSite Gateway assembly. Encodes mTagBFP under the control of a CMV promoter.DepositorInsertmTagBFP
UseMultimate/gateway entr 4ExpressionMammalianAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
1BR9-AtWUSSTOP
Plasmid#202053PurposeE. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHELDepositorInsertAtWUS E. Coli Codon Optimized (WUS Mustard Weed)
Tags6xHis and GFP11ExpressionBacterialPromoterT7 PromoterAvailable SinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
NO MS-Sensor (30)
Plasmid#199782PurposeSynthetic gene circuitDepositorInsertNO MS-Sensor
UseSynthetic BiologyAvailable SinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
NO- DSD Sensor 1
Plasmid#199785PurposeSynthetic gene circuitDepositorInsertNO- DSD Sensor
UseSynthetic BiologyAvailable SinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sfGFP-MOORmut
Plasmid#198213PurposepJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing a non-permissible minimum optimal O-linked recognition site (MOORmut) followed by a 6x-His tagDepositorInsertsfGFP_MOORmut
Tags6x His Tag and mutated (non-permissible) MOORmut …ExpressionBacterialPromoterT7Available SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG.Myc-SARS2_3CL(C145A)
Plasmid#200121PurposeExpresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL C145A in mammalian cellsDepositorInsertSARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)
TagsMycExpressionMammalianMutationC145APromoterCAGAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pcoCASphi_E9t_V2_2x35Sp_HSP18t_ribozyme_AtPDS3_gRNA10
Plasmid#197959PurposeT-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by 2x35Sp and flanked by ribozymes.DepositorInsertspcoCasphi-2
AtPDS3 gRNA10
UseCRISPRExpressionPlantPromoter2x35S promoter and pUBQ10Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pcoCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10
Plasmid#197960PurposeT-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by UBQ10 gene promoter and flanked by ribozymes.DepositorInsertspcoCasphi-2
AtPDS3 gRNA10
UseCRISPRExpressionPlantPromoterpUBQ10Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pco-nCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10
Plasmid#197980PurposeT-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by CmYLCVp, flanked by ribozymes.DepositorInsertspco-nCasphi-2
AtPDS3 gRNA10
UseCRISPRExpressionPlantPromoterCmYLCVp and pUBQ10Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Sniper2P
Plasmid#193857PurposeExpresses human codon-optimized Sniper2P and blasticidin resistance: EFS promoter-Sniper2P-NLS-FLAG-P2A-BSDDepositorInsertSniper2L
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationE1007LPromoterEFSAvailable SinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-DrCavin1b
Plasmid#194292PurposeMultisite gateway entry clone for expression of codon optimised zebrafish cavin1b with fusion tag at the N-terminus. Parton lab clone KXJDepositorInsertcavin1b (cavin1b Zebrafish)
UseMultisite gateway entry vectorAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV sgRNA-Notch1 #2 GFP
Plasmid#106951PurposeLentivirus encoding sgRNA targeting murine Notch1. sgRNA is less optimal. Includes GFP marker.DepositorAvailable SinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV sgRNA-Notch1 #3 GFP
Plasmid#106952PurposeLentivirus encoding sgRNA targeting murine Notch1. sgRNA is less optimal. Includes GFP marker.DepositorAvailable SinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::UMSBP::sNluc2_ORF1
Plasmid#186765PurposeAn mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at the endogenous gene's start codon.DepositorInsertNanoluciferase
PromoterT7Available SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::ENO::sNluc2
Plasmid#186770PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) with enolase 5' and 3' UTRsDepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::RPL15::sNluc0
Plasmid#186771PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0) with RPL15 5' and 3' UTRsDepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::RPL15::hNluc
Plasmid#186772PurposeAn mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with RPL15 5' and 3' UTRsDepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::hNluc
Plasmid#186774PurposeAn mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with y-box binding protein 5' and 3' UTRsDepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGAPDH::sNluc1
Plasmid#187221PurposeA plasmid containing a planarian codon-optimized Nluc (sNluc1), driven by the planarian GAPDH promoter.DepositorInsertNanoluciferase
UseOverexpressionPromoterGAPDHAvailable SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only