We narrowed to 165,307 results for: addgene

1. pJJiaDEST-APEX2

   Plasmid

   #149517

   Purpose

   N-termial APEX2 tag. Gateway destination vector for mammanlian expression

   Depositor

   Vojo Deretic

   Article

   Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

   Type

   Empty backbone

   Tags

   FLAG-APEX2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

   Plasmid

   #117687

   Purpose

   U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

   Depositor

   Erik Sontheimer

   Article

   Gao et al Methods Enzymol. 2019;616:365-383. doi:

   Inserts

   Kanamycin cassette
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and hPGK

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIC291

   Plasmid

   #51403

   Purpose

   Retroviral expression of EGFP-TEV-S-tag SKA1 for protein localization and affinity purification (LAP)

   Depositor

   Iain Cheeseman

   Article

   Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

   Insert

   Ska1 IMAGE 3909951 (SKA1 Human)
   

   Use

   Retroviral

   Tags

   EGFP, S tag, and TEV protease site

   Expression

   Mammalian

   Available Since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. PP1 7-300

   Plasmid

   #26566

   Depositor

   Wolfgang Peti

   Article

   Kelker et al J Mol Biol. 2009 Jan 9. 385(1):11-21.

   Insert

   PP1a (PPP1CA Human)
   

   Tags

   His6, TEV cleavage site, and Thio-6

   Expression

   Bacterial

   Mutation

   Contains only aa 7-330

   Available Since

   Dec. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pSLIK-GFP

   Plasmid

   #66844

   Purpose

   Tet-inducible expression of GFP in mammalian cells

   Depositor

   Christian Metallo

   Article

   Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. NLS-tdPCP-GFP-VPR

   Plasmid

   #183936

   Purpose

   PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   tdPCP-GFP-VPR
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV (TATA box removed)

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. CBR1_pCSdest

   Plasmid

   #53826

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   CBR1 (CBR1 Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   Sept. 9, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

8. hHS1

   Plasmid

   #159169

   Purpose

   Human codon-optimized cytosolic labile heme reporter

   Depositor

   Amit Reddi

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

   Insert

   hHS1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pBabepuroBAF180

   Plasmid

   #41078

   Depositor

   Ramon Parsons

   Article

   Xia et al Cancer Res. 2008 Mar 15;68(6):1667-74.

   Insert

   BAF180 (PBRM1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   3 silent mutations: 3595 a to g; 4981 a to t; 587…

   Available Since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCH68 (dAsCas12a-3xKRAB piggyBac)

    Plasmid

    #217334

    Purpose

    piggyBac expression of dAsCas12a-3xKRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    dAsCas12a
    

    Use

    CRISPR

    Tags

    nucleoNLS-3xKRAB-3xHA-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    E993A

    Promoter

    CAG

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCherry-STIM1

    Plasmid

    #228952

    Purpose

    Retroviral expression of mCherry-STIM1

    Depositor

    David Pellman

    Article

    Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. UPB-colon (UPB4)

    Plasmid

    #180786

    Purpose

    Unique Projection Barcode (UPB4)-Colon for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    hM3Dq ( partial )
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. BPK1520_puroR

    Plasmid

    #173901

    Purpose

    SpCas9 guide cloning vector derived from BPK1520 that allows for puromycin-based selection

    Depositor

    Ronald Cohn

    Article

    Erwood et al Genome Res. 2019 Dec;29(12):2010-2019. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV/U6

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMP597

    Plasmid

    #134999

    Purpose

    Inducible ZF-m6A Writer. Use abscisic acid (ABA) to direct GATC methylation to ZF binding sites. pMinCMV-NLS-ABI1cs-ZF-NLS-P2A-Dam(N132A)-PYL1cs-HA pGK-BlastR

    Depositor

    Ahmad Khalil

    Article

    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

    Insert

    pMinCMV-NLS-ABI1cs-ZF-NLS-P2A-Dam(N132A)-PYL1cs-HA pGK-BlastR
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMMP-dnPERK-IRES-eGFP

    Plasmid

    #36954

    Depositor

    Bill Sugden

    Article

    Lee et al Blood. 2008 Feb 15;111(4):2280-9. Epub 2

    Insert

    dnPERK (EIF2AK3 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    deletion of aa592-1061 to create dominant negative

    Promoter

    CMV

    Available Since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS_8xMmaPylT_EF1_FLAG-MmaPylRS

    Plasmid

    #174890

    Purpose

    Mma PylRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Article

    van Husen et al Chembiochem. 2021 Aug 25. doi: 10.1002/c

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    U25C PylT

    Promoter

    EF1

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMTKM05

    Plasmid

    #233477

    Purpose

    SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and Hyg selection.

    Depositor

    Vincent Martin

    Article

    Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGDP4 tet(A)

    Plasmid

    #112887

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    tet(A)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Sequence was codon optimized for E. coli K12 usin…

    Promoter

    Plac

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. CAG-Crx-IRES-GFPd2

    Plasmid

    #73997

    Purpose

    Plasmid expressing Crx-IRES- GFPd2

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    Crx-IRES-GFPd2 (Crx Mouse)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-lox-GFP shRNA p19-2

    Plasmid

    #14091

    Depositor

    Tyler Jacks

    Article

    Sage et al Nature. 2003 Jul 10. 424(6945):223-8.

    Insert

    p19 ARF shRNA -2 (Cdkn2a Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Available Since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only