We narrowed to 23,245 results for: Sis
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Plasmid#167967Purposeexpress E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E
Tagshexa-histidine tagExpressionBacterialMutationY708E, Y713E, Y715E, Y717EPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBRC901
Plasmid#167948Purposeexpress wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)DepositorInsertwild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)
Tagshexa-histidine tagExpressionBacterialPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN202
Plasmid#167950Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutationsDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations
Tagshexa-histidine tagExpressionBacterialMutationK540M, Y713E, Y715E, Y717E, Y718EPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTVKRSF225
Plasmid#135005PurposeMusF2 prenyltransferases from Nmu. PCC 7906DepositorInsertMusF2 prenyltransferaseM
TagsSUMO fusionExpressionBacterialPromoterT7Available SinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBHRSF227
Plasmid#135006PurposeMusF1 prenyltransferase from Nostoc sp. UHCC 0398DepositorInsertMusF1 prenyltransferase
TagsSUMO fusionExpressionBacterialAvailable SinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.GFP.miR-ctrl
Plasmid#169305PurposeConstitutive overexpression of miR-ctrl with GFP as a reporter fluorescent proteinDepositorInsertmiR-ctrl
UseLentiviralTagsEGFPPromoterSFFVAvailable SinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-GMRWhite
Plasmid#165885PurposeBlasticidin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screeningDepositorInsertTransgenesis Ready Selection Vector
UseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pW214-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR
Plasmid#170812PurposeLentiviral vector to co-express an sasgRNA with NLS-mScarlet-IDepositorTypeEmpty backboneUseLentiviralExpressionMammalianMutationNAAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUMO human-cPLA2-C2-domain
Plasmid#170752PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorAvailable SinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30 W96A-GLTP
Plasmid#170734PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVMG0217_Cq-U6-2b_2xBbsI-gRNA
Plasmid#169339PurposeExpression of gRNA in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0217_Cq-U6-2b_2xBbsI-gRNA
UseCRISPRExpressionInsectPromoterCPIJ039728Available SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-CVm-Sec22b
Plasmid#168029PurposeExpresses eCFP-Sec22b(A131-H190)-mVenus in bacterial cellsDepositorInserteCFP-Sec22b(A131-H190)-mVenus
TagsHisx6 and mycExpressionBacterialAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-CVm-VAMP8
Plasmid#168028PurposeExpresses eCFP-VAMP8(G7-R67)-mVenus in bacterial cellsDepositorInserteCFP-VAMP8(G7-R67)-mVenus
TagsHisx6 and mycExpressionBacterialAvailable SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPuroR-GMRWhite
Plasmid#165884PurposePuromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screeningDepositorInsertTransgenesis Ready Selection Vector
UseSynthetic BiologyAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBI-CMV1-mir374b~421-CMV2-mir1
Plasmid#164674PurposeExpress miR-374b~421 and miR-1-1 in mammalian cellsDepositorAvailable SinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBI-CMV1-mir374b-CMV2-mir1
Plasmid#164672PurposeExpress miR-374b and miR-1-1 in mammalian cellsDepositorAvailable SinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBI-CMV1-mir181b-1~125-CMV2-mir1
Plasmid#164671PurposeExpress miR-181b-1~125a and miR-1-1 in mammalian cellsDepositorAvailable SinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xQb oligo-library based
Plasmid#158201PurposeCassette of 10 different binding sites with high affinity to QB phage coat protein, as extracted from the oligo pool experiment.DepositorInsert10 binding sites of QB based on oligo library experiment
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-17
Plasmid#160717PurposeTrpC Terminator bordered by BsaI restriction sitesDepositorInserttryptophan synthase transcription terminator (TtrpC) bordered by BsaI restriction sites
UseSynthetic BiologyMutationIncorporation of BsaI restriction sitesAvailable SinceMarch 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-15
Plasmid#160715PurposeFor C-terminal tags with Trichoderma optimised Synthetic 3X-FLAG peptide bordered by BsaI restriction sitesDepositorInsertTrichoderma optimised Synthetic 3X-FLAG peptide
UseSynthetic BiologyAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-3
Plasmid#160703Purposetef1-alpha promoter from Trichoderma virens bordered by BsaI restriction sitesDepositorInserttef1-alpha
UseSynthetic BiologyMutationIncorporation of BsaI restriction sitesAvailable SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRWJB006
Plasmid#160113PurposeDestination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene freeDepositorTypeEmpty backboneUseCRISPRExpressionBacterial and PlantPromoter35SAvailable SinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRWJB004
Plasmid#160112PurposeDestination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;DepositorTypeEmpty backboneUseCRISPRExpressionBacterial and PlantPromoterUBQ10Available SinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK528
Plasmid#155388PurposedCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)DepositorInsertsdCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs
UseCRISPRExpressionBacterialMutationD917A (nuclease-deactivating)Available SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-HO-G
Plasmid#164163PurposeExpresses mEGFP (monomeric EGFP with the A206K mutation) under the control of CAG promoter, codon-optimized for Homo sapiens, basic vector, multiclonig site at C terminalDepositorInsertmEGFP A236K
ExpressionMammalianPromoterCAGAvailable SinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
p8115 LentiCRISPR v2 sgPTPN14-3
Plasmid#163314PurposeExpresses Cas9 and a human PTPN14-targeting control guide RNADepositorInsertsgPTPN14-3
UseCRISPR and LentiviralPromoterU6Available SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
HJP-178-IVS-Syn21-CreAB-NrdJ-1N-WPREw
Plasmid#162473PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreAB
UseCre/LoxAvailable SinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
HJP-208-IVS-Syn21-CreAB-NrdJ-1N-WPREUw
Plasmid#162478PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreAB
UseCre/LoxAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
HJP-195-IVS-Syn21-gp41-1C-CreB-NrdJ-1N-WPREUw
Plasmid#162476PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreB
UseCre/LoxAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
HJP-194-IVS-Syn21-CreA-gp41-1N-WPREUw
Plasmid#162475PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreA
UseCre/LoxAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
HJP-179-IVS-Syn21-NrdJ-1C-CreC-WPREw
Plasmid#162472PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreC
UseCre/LoxAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
HJP-176-IVS-Syn21-CreA-gp41-1N-WPREw
Plasmid#162470PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreA
UseCre/LoxAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
HJP-196-IVS-Syn21-NrdJ-1C-CreC-WPREUw
Plasmid#162477PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreC
UseCre/LoxAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
HJP-180-IVS-Syn21-gp41-1C-CreB-NrdJ-1N-WPREw
Plasmid#162471PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreB
UseCre/LoxAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS1HR1AtR2NtB2
Plasmid#160890PurposeExpression of N. tabacum Rubisco small subunit (rbcS-SH1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-S1H
Tags6xHisExpressionBacterialPromoterrbcS-SH1: T7, chaperones: T13Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtT4aR1AtR2NtB2
Plasmid#160887PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T4a), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T4a
ExpressionBacterialPromoterrbcS-T4a: T7, chaperones: T10Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE669
Plasmid#153235PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceOct. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDUAL-Pnmt1-Ost4-GFP
Plasmid#154368PurposeYeast expression of Ost4DepositorInsertOst4 (ost4 Fission Yeast)
ExpressionYeastAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE672
Plasmid#153238PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsGFPExpressionPlantAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15-His-∆NGlpG(Y205F)
Plasmid#155132PurposeExpresses N-terminally His-tagged E. coli GlpG Y205F variant (residues 87-276) from pET15bDepositorInsertGlpG (glpG Escherichia coli)
TagsHisExpressionBacterialMutationY205F, insert codes for GlpG residues 87-276PromoterT7Available SinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE473
Plasmid#153264PurposeM1E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE500
Plasmid#153273PurposeM6E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE502
Plasmid#153275PurposeM8E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Myosin7B-sgRNA
Plasmid#154861PurposeLentiviral expression of Cas9 and sgRNA targeting Myosin7BDepositorAvailable SinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAL-C2X dAmp/Kana+-2MT
Plasmid#154353PurposeBacterial expression of 2MTDepositorInsert2MT
TagsHisExpressionBacterialAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDUAL-P41nmt1-Sad1-GFP
Plasmid#154366PurposeYeast expression of Sad1DepositorInsertSad1 (sad1 Fission Yeast)
ExpressionYeastAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDUAL-P41nmt1-Nup124-GFP
Plasmid#154364PurposeYeast expression of Nup124DepositorInsertNup124 (nup124 Fission Yeast)
ExpressionYeastAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDUAL-Pnmt1-Ost4-GFP-MTalpha
Plasmid#154370PurposeYeast expression of Ost4DepositorInsertOst4 (ost4 Fission Yeast)
ExpressionYeastAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDUAL-P41nmt1-Sad1-GFP-MTn
Plasmid#154367PurposeYeast expression of Sad1DepositorInsertSad1 (sad1 Fission Yeast)
ExpressionYeastAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-tglA(C51)HIB
Plasmid#134675PurposeExpresses His6-TglA(C51), TglH, TglI and TglB in E. ColiDepositorInsertsTglA(C51)
TglH
TglI
TglB
TagsHis6ExpressionBacterialMutationinsertion of cysteine 51PromoterT7Available SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only