We narrowed to 22,939 results for: REP

Showing: 4741 - 4760 of 22939 results

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A3Bi-Cas9n-UGI

Plasmid

#119135

Purpose

Base editor made from full-length APOBEC3B with an L1 intron to prevent self-restriction

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3B L1 intron-Cas9 nickase-UGI

Use

CRISPR

Mutation

L1 intron insertion

Promoter

CMV

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR2B-dCas9-KRAB

Plasmid

#115547

Purpose

Entry cassette for dCas9-KRAB

Depositor

Rene Maehr

Article

Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

Insert

dCas9-KRAB

Tags

HA

Expression

Bacterial and Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3 ss-FLAG-AP-hNDP (aa 25-133)

Plasmid

#115789

Purpose

Expresses and secretes human Norrin with N-terminal flag and alkaline phosphatase tags, contains artificial signal sequence upstream of tags.

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

NDP (NDP Human)

Tags

alkaline phosphatase and flag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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LT3GEN

Plasmid

#111173

Purpose

Tet-regulated miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Promoter

T3G

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA

Plasmid

#105000

Purpose

Expresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

Depositor

Jeffrey Chao

Article

Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

Insert

Renilla luciferase

Tags

24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

Expression

Bacterial and Mammalian

Promoter

TetCMV

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.5 shETV5-A

Plasmid

#74977

Purpose

shETV5 shRNA

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ETV5 (ETV5 Human)

Use

RNAi

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2 Pc-gE

Plasmid

#51256

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Glu

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 216,220,224,230,248 mutated to Glu

Promoter

EF1a

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-pCALM1-EYFP-WPRE-bGHpA

Plasmid

#213971

Purpose

AAV expression of EYFP from pCALM1 promoter

Depositor

Zhonghua Lu

Article

Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

Insert

pCALM1

Use

AAV

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPGC1a_Proximal Promoter-FLuc2P

Plasmid

#154259

Purpose

Destabilized firefly luciferase reporter containing the Human PGC-1alpha Proximal Promoter from -500 to +52.

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

PGC-1a Proximal Promoter (PPARGC1A Human)

Expression

Mammalian

Promoter

Human PGC-1alpha Proximal Promoter

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_FLAG-AbKRS-chIPYE

Plasmid

#140020

Purpose

Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

AbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)

Tags

FLAG

Expression

Mammalian

Mutation

1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…

Promoter

EF1

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-Puro-{CMV-CuO}>{PAX3::FOXO1}:T2A:mCherry

Plasmid

#236629

Purpose

Puromycin selected lentiviral construct with cumate-inducible PAX3::FOXO1

Depositor

Genevieve Kendall

Article

Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

Inserts

PAX3::FOXO1 (FOXO1, PAX3 Human)
mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-CuO

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pIS1_Opt_Renilla

Plasmid

#210571

Purpose

Expresses Optimal Renilla luciferase

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Optimal Renilla

Use

Luciferase

Expression

Mammalian

Promoter

HSV TK; T7

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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PGK EGFP-PPM1H-WT

Plasmid

#200084

Purpose

Mammalian expression of wild-type PPM1H

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

PPM1H (PPM1H Human)

Tags

EGFP

Expression

Mammalian

Promoter

PGK

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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PGK CFP

Plasmid

#200429

Purpose

Mammalian expression of CFP

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

CFP

Expression

Mammalian

Promoter

PGK

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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A1-20B1 (P001)

Plasmid

#161820

Purpose

This plasmid carries Yn situ preamplifier A1-20B1 sequence.

Depositor

C. Ron Yu

Article

Wu et al Cell Rep Methods. 2022 Apr 18;2(4):10020

Insert

A1-20B1

Use

Synthetic Biology

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pNIC-CFP

Plasmid

#173074

Purpose

Expression vector for E. coli producing target protein with N-terminal CFP fusion

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Type

Empty backbone

Tags

mCerulean

Expression

Bacterial

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-DD-Hygro

Plasmid

#175267

Purpose

MegaGate destination with C-terminal DD tag and hygromycin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Tags

Destabilization Domain

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Ssh1

Plasmid

#163602

Purpose

Expresses Protein phosphatase Slingshot homolog 1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pHR Src-TagBFP

Plasmid

#171036

Purpose

TagBFP-tagged Src kinase

Depositor

Jared Toettcher

Article

Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

Insert

Src

Use

Lentiviral

Expression

Mammalian

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-Y419F

Plasmid

#140317

Purpose

Expresses Flag-tagged human Src-Y419F in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

Y419F

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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