We narrowed to 9,856 results for: UTY

1. pLCKO_PSMD1_sgRNA_5

   Plasmid

   #74181

   Purpose

   lentiviral vector expressing sgRNA targeting PSMD1

   Depositor

   Jason Moffat

   Article

   Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

   Insert

   PSMD1 sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 Promoter

   Available Since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNAIIIB rac1 QL

   Plasmid

   #61633

   Purpose

   mammalian expression of rac1 constitutively active mutant

   Depositor

   JS Gutkind

   Article

   Coso et al Cell. 1995 Jun 30;81(7):1137-46.

   Insert

   Rac1 (RAC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q61L, constitutively active

   Promoter

   CMV

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

3. BCL9-BirA*

   Plasmid

   #109147

   Purpose

   To generate stable cell lines expressing BCL9-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells

   Depositor

   Mariann Bienz

   Article

   van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife

   Insert

   BCL9 (BCL9 Human)
   

   Use

   Tags

   BirA(R118G)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO2

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSF11-wmiRFP2-T2A-HO1

   Plasmid

   #179485

   Purpose

   Expresses miRFP2 and HO1in worm cells

   Depositor

   Kiryl Piatkevich

   Article

   Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

   Insert

   wmIRFP2-T2A-HO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NO

   Promoter

   tag-168 promoter

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEMS1725

   Plasmid

   #105870

   Purpose

   High copy number plasmid containing iCreERT2-FFrt-KanR-FFrt insert

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

   Insert

   iCreERT2-FFrt-KanR-FFrt
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLCKO_PSMD1_sgRNA_1

   Plasmid

   #74180

   Purpose

   lentiviral vector expressing sgRNA targeting PSMD1

   Depositor

   Jason Moffat

   Article

   Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

   Insert

   PSMD1 sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 Promoter

   Available Since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FUG-T2A-Cas9

   Plasmid

   #75346

   Purpose

   Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif. For cell transfection or use in lentiviral packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

   Depositor

   Bryan Luikart

   Article

   Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP (via t2a)

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-FAM86A

   Plasmid

   #85117

   Purpose

   bacterial expression of FAM86A

   Depositor

   Pal Falnes

   Article

   Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

   Insert

   EEF2KMT (FAM86A Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNCST-AausFP2

   Plasmid

   #129500

   Purpose

   Expresses AausFP2 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AausFP2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

    Plasmid

    #124660

    Purpose

    reporter protein which emits bright blue fluorescence

    Depositor

    Yutaka Kuroda

    Article

    Wu et al Sci Rep. 2020 Nov 18;10(1):20069. doi: 1

    Insert

    Gaussia luciferase-C9D-TGinsert
    

    Use

    Tags

    IEGRTMGDDDGDDDGDDD（FactorXa,TMG,C9D-Tag） and MHHH…

    Expression

    Bacterial

    Mutation

    changed glutamic acid 100 to alanine, and glycine…

    Promoter

    T7 promoter

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. tet-pLKO shMKP5.v2 puro

    Plasmid

    #87793

    Purpose

    Expresses an inducible short hairpin targeting human MKP5 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP5 (DUSP10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE tight

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. tet-pLKO shMKP5.v1 puro

    Plasmid

    #87792

    Purpose

    Expresses an inducible short hairpin targeting human MKP5 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP5 (DUSP10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE tight

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNCST-AausGFP

    Plasmid

    #129503

    Purpose

    Expresses AausGFP constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AausGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FKBP-Stx3-mCitrine

    Plasmid

    #92428

    Purpose

    Expression of syntaxin-3 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    Stx3 (Stx3 Rat)
    

    Use

    Tags

    FKBP (FK506 binding protein 12); allows for heter…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST47-CYFIP2.GFP

    Plasmid

    #109140

    Purpose

    GFP-tagged CYFIP2 expression construct

    Depositor

    Josef Kittler

    Article

    Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

    Insert

    CYFIP2 (CYFIP2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FKBP-Stx4-mCitrine

    Plasmid

    #92427

    Purpose

    Expression of syntaxin-4 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    Stx4 (Stx4 Rat)
    

    Use

    Tags

    FKBP (FK506 binding protein 12); allows for heter…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28-mTDGa.1

    Plasmid

    #81051

    Purpose

    bacterial expression of catalytically dead murine TDG

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TDG (Tdg Mouse)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    asparagine 151 changed to alanine

    Promoter

    T7

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCapV

    Plasmid

    #183028

    Purpose

    the patatin-like phosphodiesterase CapV from the animal commensal strain Escherichia coli ECOR31 cloned in the L-arabinose inducible vector pBAD28

    Depositor

    Ute Romling

    Article

    Li et al NPJ Biofilms Microbiomes. 2022 May 11;8(

    Insert

    patatin-like phospholipase CapV
    

    Use

    Tags

    No

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD promoter

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Hy_pMT Flag-Hel25E K91N

    Plasmid

    #110121

    Purpose

    Expresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Hel25E (Hel25E Fly)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    K91N mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Hy_pMT Flag-Hel25E D193E

    Plasmid

    #110120

    Purpose

    Expresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Hel25E (Hel25E Fly)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    D193E mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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