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pDAC565

Plasmid

#195242

Purpose

Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)

Depositor

Jennifer Doudna

Publication

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA

Expression

Mammalian

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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lentiCRISPRi(v2)-Blast

Plasmid

#170068

Purpose

Expresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistance

Depositor

Neville Sanjana

Publication

Morris et al Science. 2023 May 19;380(6646):eadh7699.

Insert

KRAB-dCas9-MeCP2

Use

CRISPR and Lentiviral

Tags

2A and 2xHA tag

Expression

Mammalian

Promoter

EFS-NS

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMD2-VSVGmut

Plasmid

#182229

Purpose

Expresses VSVGmut protein in mammalian cells for lentiviral pseudotyping

Depositor

Michael Birnbaum

Publication

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

VSVGmut

Expression

Mammalian

Mutation

K47Q, R354A

Promoter

CMV

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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scFLARE2

Plasmid

#158700

Purpose

Neuronal expression of scFLARE2 component

Depositor

Alice Ting

Publication

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

scFLARE2

Use

AAV

Tags

HA, Flag, VP16

Expression

Mammalian

Mutation

Truncated form of TEV protease (220-242 amino aci…

Promoter

Syn

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pXG237

Plasmid

#141281

Purpose

ATF4 translational reporter

Depositor

Martin Kampmann

Publication

Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

Insert

uORF1/2 of ATF4 followed by mApple

Use

Lentiviral

Tags

mApple

Promoter

CMV

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-S5E2-dTom-nlsdTom

Plasmid

#135630

Purpose

AAV vector to drive the expression of dTomato in PV cortical interneurons under the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Publication

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has service

AAV1, AAV9, and AAV PHP.eB

Insert

dTom-nlsdTom

Use

AAV

Mutation

None

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pTP264

Plasmid

#149334

Purpose

Bacterial expression of Biotin ligase BirA

Depositor

Dirk Görlich

Publication

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Biotin ligase BirA

Tags

His14 and bdNEDD8

Expression

Bacterial

Available since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA3 TFAM-mScarlet

Plasmid

#129573

Purpose

Expression of mScarlet fused TFAM

Depositor

Stephen Tait

Publication

Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TFAM (TFAM Human)

Expression

Mammalian

Mutation

None

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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PB-U6insert-EF1puro

Plasmid

#104537

Purpose

A piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAs

Depositor

George Church

Publication

Kalhor et al Science. 2018 Aug 9. pii: science.aat980

Type

Empty backbone

Use

CRISPR, Mouse Targeting, and Synthetic Biology ; …

Expression

Mammalian

Promoter

U6

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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EGFP-BAF

Plasmid

#101772

Purpose

Expresses EGFP tagged BAF in human cells

Depositor

Daniel Gerlich

Publication

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pHR-Tre3G-47xCAG-12xMS2

Plasmid

#99148

Purpose

Encodes 47xCAG repeats with 12xMS2 tag, 2nd generation lentiviral vector

Depositor

Ron Vale

Publication

Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

Insert

47xCAG 12xMS2 hairpins

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

Plasmid

#92392

Purpose

produces AAV expressing Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

Depositor

Hyungbae Kwon

Publication

Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

Human Synapsin

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Ca-FLARE (TF)

Plasmid

#92213

Purpose

Transcription factor component of FLARE

Depositor

Alice Ting

Publication

Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

NRX-TM-Nav1.6 -CaMbp(M2)-eLOV-TEVcs(ENLYFQ▲M)-tTA-VP16

Use

AAV

Expression

Mammalian

Promoter

synapsin

Available since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

Plasmid

#85452

Purpose

Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.

Depositor

Aviv Regev

Publication

Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

Insert

U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro

Use

CRISPR and Lentiviral

Tags

NLS

Expression

Mammalian

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMJ117

Plasmid

#85997

Purpose

One-guide Perturb-seq vector backbone; modified human U6 promoter; constant region 3

Depositor

Jonathan Weissman

Publication

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

EGFP-NT2_cr3
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified human U6

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pX601-mCherry

Plasmid

#84039

Purpose

Staphylococcus aureus (SaCas9) conjugated with mCherry

Depositor

Yuet Wai Kan

Publication

Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

Insert

SaCas9

Use

AAV and CRISPR

Tags

NLS and T2A-mCherry

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pAAVS1-P-CAG-mCh

Plasmid

#80492

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expression

Depositor

Knut Woltjen

Publication

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry

Use

Donor vector (human)

Expression

Mammalian

Promoter

CAG

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLentiCas9-T2A-GFP

Plasmid

#78548

Purpose

Cas9 protein with GFP

Depositor

Roderic Guigo, Rory Johnson

Publication

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

T2A-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

In frame with Cas9

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHRm-GFP(1-10)-VP64-NLS

Plasmid

#70228

Purpose

Expresses GFP(1-10)-VP64-NLS in mammalian cells

Depositor

Bo Huang

Publication

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP(1-10)-VP64-NLS

Use

Lentiviral

Expression

Mammalian

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSTARR-seq_human

Plasmid

#71509

Purpose

humanSTARR-seq screening vector with SCP1 core promoter

Depositor

Alexander Stark

Publication

Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

Type

Empty backbone

Use

Humanstarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Mammalian

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles