165,343 results

1. pD649-HAsp-EDAR-COMP5AP-AviTag-9xHis

   Plasmid

   #157119

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   EDAR (EDAR Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pD649-HAsp-EDA2R-COMP5AP-AviTag-9xHis

   Plasmid

   #157096

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   EDA2R (EDA2R Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pD649-HAsp-CEACAM6-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156723

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   CEACAM6 (CEACAM6 Human)
   

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pD649-HAsp-CEACAM5-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156937

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   CEACAM5 (CEACAM5 Human)
   

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available Since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pD649-HAsp-CEACAM8-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156730

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   CEACAM8 (CEACAM8 Human)
   

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available Since

   Jan. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Full-length WT talin1

   Plasmid

   #182992

   Purpose

   express FL WT talin1 in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

   Insert

   Talin1 WT (Tln1 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(-) HuTF-Fc-His tag

   Plasmid

   #183253

   Purpose

   Soluble expression of the extracellular domains of human tissue factor (TF)-Fc

   Depositor

   Alexander McLellan

   Insert

   Tissue factor (F3 Human)
   

   Tags

   8xHis

   Expression

   Mammalian

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15b MOSPD2 [282-490]

   Plasmid

   #186469

   Purpose

   Expression of human MOSPD2 (MSP domain from residue 282 to 490), fused to a 6HIS tag, in bacteria

   Depositor

   Catherine Tomasetto

   Article

   Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

   Insert

   MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
   

   Tags

   6HIS

   Expression

   Bacterial

   Mutation

   MSP domain [282-490]

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJOG299

   Plasmid

   #105360

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   promotor AP1 (At1g69120;1400bp)
   

   Use

   Synthetic Biology

   Mutation

   BsaI/ BpiI restriction sites removed

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. CL20_mEGFP_ASPSCR1_TFE3

    Plasmid

    #205776

    Purpose

    Express mEGFP-tagged fusion protein, ASPSCR1_TFE3 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    ASPSCR1_TFE3 (ASPSCR1, TFE3 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR_Pax6-RE_tBFP_PGK_mCitrine

    Plasmid

    #188388

    Purpose

    Lentiviral vector - tBFP reporter for Pax6-based SNIPRs with a constitutive mCitrine

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    Pax6-RE_tBFP_PGK_mCitrine
    

    Use

    Lentiviral

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA C12 ecDHFR DD YFP HA

    Plasmid

    #192815

    Purpose

    Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.

    Depositor

    John Hulleman

    Article

    Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021

    Insert

    E. coli dihydrofolate reductase
    

    Tags

    enhanced yellow fluorescent protein and hemagglut…

    Expression

    Mammalian

    Mutation

    W74R/T113S/E120D/Q146L

    Promoter

    CMV

    Available Since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJH001

    Plasmid

    #67641

    Purpose

    High copy Cas9 expression vector with LEU2 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    Empty
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMTU-DO-HPH-C4

    Plasmid

    #160343

    Purpose

    High-copy expression vector for K. marxianus, hygromycin selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Expression

    Yeast

    Promoter

    n/a

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKM208

    Plasmid

    #13077

    Depositor

    Kenan Murphy

    Article

    Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

    Insert

    lacI repressor (lacI E. coli)
    

    Expression

    Bacterial

    Available Since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. IDG_CACNB2_OE_1

    Plasmid

    #161684

    Purpose

    Overexpresses CACNB2

    Depositor

    Mike McManus

    Insert

    CACNB2 (CACNB2 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCX138

    Plasmid

    #229213

    Purpose

    CRISPR array plasmid for NOTCH2 (Spacers 25-48)

    Depositor

    Jennifer Doudna

    Article

    Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

    Insert

    CRISPR array plasmid for NOTCH2 (Spacers 25-48)
    

    Expression

    Mammalian

    Available Since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-MET

    Plasmid

    #116762

    Purpose

    Lentiviral expression of MET

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    MET (MET Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMKO.1 GFP

    Plasmid

    #10676

    Purpose

    Retroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.

    Depositor

    William Hahn

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAKTaq

    Plasmid

    #25712

    Purpose

    Bacterial expression of Taq polymerase

    Depositor

    David Engelke

    Article

    Engelke et al Anal Biochem. 1990 Dec . 191(2):396-400.

    Insert

    Taq polymerase
    

    Expression

    Bacterial

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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