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pXCX.CMV.EGFP.WPRE

Plasmid

#38055

Depositor

James Uney

Article

Glover et al Mol Ther. 2002 May;5(5 Pt 1):509-16.

Insert

EGFP

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pCfB8740

Plasmid

#126906

Purpose

Template for PCR amplification of XdCrtI

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI

Use

Synthetic Biology

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB8741

Plasmid

#126907

Purpose

Template for PCR amplification of XdCrtE

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtE

Use

Synthetic Biology

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pSG 3/4

Plasmid

#63868

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UGA near N-term

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pSG 853

Plasmid

#63873

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UAA near 5' end

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pSG 163

Plasmid

#63871

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UAG near 5' end

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pCS2+8NCerulean-ABCB1a

Plasmid

#34944

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCB1a

Use

Sea urchin, zebrafish, xenopus

Tags

Cerulean

Expression

Mammalian

Promoter

SP6

Available Since

Jan. 7, 2013

Availability

Academic Institutions and Nonprofits only
LV UAS-DsRed-Express2-PGK-Puro

Plasmid

#190801

Purpose

LV construct encoding UAS-DsRed-Express2 reporter gene.

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

UAS-T2A-DsRed-Express2

Use

Lentiviral and Synthetic Biology

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSW104

Plasmid

#122295

Purpose

For run-off transcription of yeast RPL41A mRNA with and without an 82 nucleotide poly(A) tail.

Depositor

Sarah Walker

Article

Liu et al Methods. 2019 Mar 26. pii: S1046-2023(18

Insert

RPL41A

Use

In vitro transcription

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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Cas9m3

Plasmid

#47315

Purpose

Cas9 D10A+D839A+H840A

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

Cas9m3

Use

CRISPR

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pGL2-ptc-lu

Plasmid

#37678

Depositor

Philip Beachy

Article

Chen et al Cell. 1999 Aug 6;98(3):305-16.

Insert

ptc promoter (−758 to +130)

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

ptc

Available Since

Nov. 12, 2012

Availability

Academic Institutions and Nonprofits only
PD13

Plasmid

#164530

Purpose

Expresses HA-Ub noK

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Ubiquitin noK

Use

Lentiviral

Tags

HA and Ub noK

Mutation

All seven Lysines of Ubiquitin changed to Arginine

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE-FLAG-hG9a

Plasmid

#33023

Depositor

Martin Walsh

Article

Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

Insert

hG9a

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

5'LTR

Available Since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
pJW1504

Plasmid

#62358

Purpose

RPS2 HA-TEV-AVI pop-in/pop-out plasmid

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

RPS2 fragment

Use

Yeast targeting

Tags

HA-TEV-AVI

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2+8NmCherry-ABCC1

Plasmid

#34942

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCC1

Use

Sea urchin, zebrafish, xenopus

Tags

mCherry

Expression

Mammalian

Promoter

SP6

Available Since

Jan. 7, 2013

Availability

Academic Institutions and Nonprofits only
pEF/myc/ER FLIPglu-3.2mDelta13V

Plasmid

#18019

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16A S112A Delta13

Tags

ECFP and Venus

Expression

Mammalian

Mutation

F16A, S112A in binding protein

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pIBG-FTet

Plasmid

#33361

Depositor

Doug Mortlock

Article

Chandler et al Mol Cell Biol. 2007 Apr;27(8):2934-51. E

Inserts

IRES-Bgeo
TetR cassette

Use

Mouse Targeting

Tags

FRT site

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pEnt L1L3 tTA-3

Plasmid

#27105

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

tetracycline transactivator

Use

Gateway entry vector

Available Since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pSG lac10

Plasmid

#63875

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

-1 frameshift near 5' end

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
B3-4

Plasmid

#111474

Purpose

translational release factor 2

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational release factor 2

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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