We narrowed to 1,593 results for: PTS

1. pCIneo-lambdaN-HA-HsTNRC6A-delMII_F

   Plasmid

   #146297

   Purpose

   Mammalian Expression of HsTNRC6A-delMII

   Depositor

   Elisa Izaurralde

   Article

   Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

   Insert

   HsTNRC6A-delMII (TNRC6A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one non silent mutation K1578E compared to the se…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCIneo-lambdaN-HA-HsTNRC6A-C-term_F

   Plasmid

   #146294

   Purpose

   Mammalian Expression of HsTNRC6A-Cterm

   Depositor

   Elisa Izaurralde

   Article

   Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

   Insert

   HsTNRC6A-Cterm (TNRC6A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one non silent mutation K1578E compared to the se…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pN1-HsSmg5-del133-155-V5-SBP-MBP_V

   Plasmid

   #147763

   Purpose

   Mammalian Expression of HsSmg5-del133-155

   Depositor

   Elisa Izaurralde

   Article

   Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

   Insert

   HsSmg5-del133-155 (SMG5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one silent mutation C969T compared to the sequenc…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pN1-HsSmg5-del267-809-V5-SBP-MBP_V

   Plasmid

   #147764

   Purpose

   Mammalian Expression of HsSmg5-del267-809

   Depositor

   Elisa Izaurralde

   Article

   Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

   Insert

   HsSmg5-del267-809 (SMG5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one silent mutation C969T compared to the sequenc…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pN1-HsSmg5-del810-846-V5-SBP-MBP_V

   Plasmid

   #147765

   Purpose

   Mammalian Expression of HsSmg5-del810-846

   Depositor

   Elisa Izaurralde

   Article

   Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

   Insert

   HsSmg5-del810-846 (SMG5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one silent mutation C969T compared to the sequenc…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pN1-HsSmg5-del847-1017-V5-SBP-MBP_V

   Plasmid

   #147766

   Purpose

   Mammalian Expression of HsSmg5-del847-1017

   Depositor

   Elisa Izaurralde

   Article

   Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

   Insert

   HsSmg5-del847-1017 (SMG5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one silent mutation C969T compared to the sequenc…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-MS2-HA-HsSmg5-del133-155-G120E_T

   Plasmid

   #147554

   Purpose

   Mammalian Expression of HsSmg5-del133-155-G120E

   Depositor

   Elisa Izaurralde

   Article

   Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

   Insert

   HsSmg5-del133-155-G120E (SMG5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one silent mutation compared to the sequence give…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. UG76-pLYS2-GFP-PKCbeta

   Plasmid

   #184762

   Purpose

   Marker for DAG. Yeast expression of human PKC beta under LSY2 promoter. Uses auxotrophic marker TRP1(Kluyveromyces lactis).

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   pLYS2-GFP-PKCbeta (PRKCB Human, Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. dora[B]_rescue_construct

   Plasmid

   #190637

   Purpose

   Puromycin-selectable expression of Dora[T295M] (CG34401) in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Dorado (CG34401 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Changed threonine 295 to methionine

   Promoter

   Actin-5c

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HA-dora[B]_rescue_construct

    Plasmid

    #190638

    Purpose

    Puromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Use

    Tags

    3xHA

    Expression

    Insect

    Mutation

    Changed threonine 295 to methionine

    Promoter

    Actin-5c

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. dora[A]_rescue_construct

    Plasmid

    #190639

    Purpose

    Puromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Truncated to contain amino acids 1-945

    Promoter

    Actin-5c

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HA-dora[A]_rescue_construct

    Plasmid

    #190640

    Purpose

    Puromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Use

    Tags

    3xHA

    Expression

    Insect

    Mutation

    Truncated to contain amino acids 1-945

    Promoter

    Actin-5c

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pN1-HsSmg5-G120E-del133-155-V5-SBP-MBP_V

    Plasmid

    #147767

    Purpose

    Mammalian Expression of HsSmg5-G120E-del133-155

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsSmg5-G120E-del133-155 (SMG5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation C969T compared to the sequenc…

    Promoter

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pN1-HsSmg5-G120E-siRNAres-V5-SBP-MBP_S

    Plasmid

    #147470

    Purpose

    Mammalian Expression of HsSmg5-G120E-siRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

    Insert

    HsSmg5-G120E-siRNAres (SMG5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation C969T compared to the sequenc…

    Promoter

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTH862-CEN-slowmaxRLuc/minCFLuc

    Plasmid

    #115371

    Purpose

    Expresses translation initiation-controlled Renilla luciferase and translation elongation-controlled firefly luciferase

    Depositor

    Tobias von der Haar

    Inserts

    Firefly Luciferase (codon minimised)
    Gcn4 5'-UTR + Renilla luciferase (codon optimised)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    All codons have been changed for the most favoura…

    Promoter

    ADH1 and TDH3

    Available since

    June 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21a - Ec-ClpX D144L

    Plasmid

    #111514

    Purpose

    Expresses E. coli ClpX with a C-terminal His tag and a mutation from D144 to L144

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

    Insert

    ClpX
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Aspartatic acid 144 to Leucine

    Promoter

    T7

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p198 Dync1i2.F (Ex1a)

    Plasmid

    #26448

    Depositor

    Elizabeth Fisher

    Article

    Kuta et al PLoS One. 2010 . 5(7):e11682.

    Insert

    cytoplasmic dynein 1 intermediate chain 2 isoform F (exon 1a) (Dync1i2 Mouse)
    

    Use

    Subcloning and sequencing

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    March 24, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pmGFP-ADAR1-p150

    Plasmid

    #117927

    Purpose

    ADAR1-p150 expression plasmid.

    Depositor

    Kumiko Ui-Tei

    Article

    Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

    Insert

    ADAR1-p150 (ADAR Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lentiviral-S-tdTomato

    Plasmid

    #112579

    Purpose

    Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

    Depositor

    Jeffrey Rothstein

    Article

    Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

    Insert

    NLS-tdTomato-NES
    

    Use

    Lentiviral

    Tags

    nuclear export sequence and nuclear localization …

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term

    Plasmid

    #182925

    Purpose

    Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Type

    Empty backbone

    Use

    Tags

    APEX2 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA5-FRT/TO-3*Flag-APEX2 C-term 3NLS

    Plasmid

    #187588

    Purpose

    Express FLAG epitope and APEX2-tagged 3xNLS fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    NLS
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDEST-cDNA5-FRT/TO-3*Flag-APEX2 C-term

    Plasmid

    #182926

    Purpose

    Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Type

    Empty backbone

    Use

    Tags

    APEX2 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pmGFP-ADAR1-p110

    Plasmid

    #117928

    Purpose

    ADAR1-p110 expression plasmid.

    Depositor

    Kumiko Ui-Tei

    Article

    Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

    Insert

    ADAR1-p110 (ADAR Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pIRES2_EGFP_KCNQ1

    Plasmid

    #173161

    Purpose

    For mammalian expression of KCNQ1 and creating variants

    Depositor

    Al George

    Article

    Vanoye et al Circ Genom Precis Med. 2018 Nov;11(11):e

    Insert

    KCNQ1 (KCNQ1 Human)
    

    Use

    Tags

    IRES2-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLentiRNAGuide_003

    Plasmid

    #192505

    Purpose

    For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFP

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

    Insert

    hU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PGK EGFP-PPM1H-WT

    Plasmid

    #200084

    Purpose

    Mammalian expression of wild-type PPM1H

    Depositor

    Erika Holzbaur

    Article

    Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

    Insert

    PPM1H (PPM1H Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFRT/TO/HIS/FLAG/HA-PRDX1

    Plasmid

    #38086

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    PRDX1 peroxiredoxin 1 (PRDX1 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PGK ARF6(Q67L)-CFP

    Plasmid

    #200428

    Purpose

    Mammalian expression of Q67L mutation in ARF6

    Depositor

    Erika Holzbaur

    Article

    Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

    Insert

    ARF6 (ARF6 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Q67L

    Promoter

    PGK

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. canxTM-mEmerald (cytosolic)

    Plasmid

    #186962

    Purpose

    Expresses mEmerald connected with the C-terminus of Calnexin transmembrane domain.

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    CANX (CANX Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Only express calnexin (482-end) and add ER signal…

    Promoter

    CMV Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFRT/TO/HIS/FLAG/HA-SART1

    Plasmid

    #38087

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    SART1 squamous cell carcinoma antigen recognized by T cells (SART1 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. mEmerald(luminal)-canxTM

    Plasmid

    #186961

    Purpose

    Expresses mEmerald connected with the N-terminus of Calnexin transmembrane domain.

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    CANX (CANX Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Only express calnexin (482-end) with ER signal pe…

    Promoter

    CMV Promoter

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFRT/TO/HIS/FLAG/HA-MYEF2

    Plasmid

    #38069

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    MYEF2 myelin expression factor 2 (MYEF2 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRK5 Fyn deltaSH3

    Plasmid

    #16034

    Depositor

    Filippo Giancotti

    Article

    Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

    Insert

    c-Fyn (FYN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Delta SH3: deletion of amino acids 76-141

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

34. pRK5 Fyn SH2:R176E

    Plasmid

    #16035

    Depositor

    Filippo Giancotti

    Article

    Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

    Insert

    c-Fyn (FYN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SH2 point mutant: R176E

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

35. pCS2-mqgDUX4mqg*

    Plasmid

    #21177

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Apr 9. ():.

    Insert

    DUX4 (DUX4 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Point mutation at nucleotide 6163 (numbering acco…

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

36. lentiGuideFB-Puro-A

    Plasmid

    #192506

    Purpose

    For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a nd a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

    Insert

    hU6-sgRNA-CS1-BsmBI-EFS-Puro-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. lentiGuideFB-Puro-B

    Plasmid

    #192507

    Purpose

    For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

    Insert

    bU6-sgRNACR2-CS-BsmBI-EFS-Puro-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    bU6

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCS2-mkgDUX4mqg*

    Plasmid

    #21175

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Apr 9. ():.

    Insert

    DUX4 (DUX4 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Point mutation at nucleotide 6163 (numbering acco…

    Promoter

    Available since

    Aug. 6, 2009

    Availability

    Academic Institutions and Nonprofits only

39. pCS2-mkgDUX4mal*

    Plasmid

    #21172

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Apr 9. ():.

    Insert

    DUX4 (DUX4 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Point mutation at nucleotide 5114 (numbering acco…

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

40. P63-sp64 Poly A

    Plasmid

    #59435

    Purpose

    synthetic poly A transcripts of VZV

    Depositor

    Randall Cohrs

    Article

    Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

    Insert

    VZV ORF 63
    

    Use

    Makes synthetic transcripts that contains poly a

    Tags

    none

    Expression

    Mutation

    none

    Promoter

    SP6

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

41. P29-sp64 Poly A

    Plasmid

    #59434

    Purpose

    synthetic poly A transcripts of VZV

    Depositor

    Randall Cohrs

    Article

    Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

    Insert

    VZV ORF 29
    

    Use

    Makes synthetic transcripts that contains poly a

    Tags

    none

    Expression

    Mutation

    none

    Promoter

    SP6

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

42. P21-sp64 Poly A

    Plasmid

    #59433

    Purpose

    synthetic poly A transcripts of VZV

    Depositor

    Randall Cohrs

    Article

    Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

    Insert

    VZV ORF 21
    

    Use

    Makes synthetic transcripts that contains poly a

    Tags

    none

    Expression

    Mutation

    none

    Promoter

    SP6

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

43. pCA528 huPQBP1

    Plasmid

    #217449

    Purpose

    Expresses full-length human PQBP1 from a His-SUMO bacterial expression vector

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    PQBP1 (PQBP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. HIV-1-GFP ΔEnv CA E45A

    Plasmid

    #217438

    Purpose

    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with E45A mutation in CA).

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    gag/pol
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    CA E45A

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. HIV-1-GFP ΔEnv CA M66I

    Plasmid

    #217440

    Purpose

    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with M66I mutation in CA).

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    gag/pol
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    CA M66I

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. SG3ΔENV CA Q67H/N74D

    Plasmid

    #217442

    Purpose

    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and Q67H/N74D mutations in capsid (CA Q67H/N74D).

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    gag/pol
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    CA Q67H,N74D

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

    Plasmid

    #200793

    Purpose

    Mammalian expression of CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

    Depositor

    Alison Twelvetrees

    Article

    Smith et al bioRxiv 2024.12.20.629623

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    CLIPf and SNAPf, HisTag, FLAG

    Expression

    Mammalian

    Mutation

    1-914; Δ505-610

    Promoter

    CMV & T7

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pLenti-rTetR(SE)-EED-2A-mTurquoise

    Plasmid

    #225692

    Purpose

    Lentiviral expression of rTetR(SE) fused to EED and mTurquoise-NLS

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    rTetR-EED (EED Human, Synthetic)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xFLAG and T2A-mTurquoise

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pLenti-rTetR(SE)-HP1a-2A-mTurquoise

    Plasmid

    #225691

    Purpose

    Lentiviral expression of rTetR(SE) fused to HP1a and mTurquoise-NLS

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    HP1a (CBX5 Human, Synthetic)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xFLAG and T2A-mTurquoise

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pN1-HsSmg5-Q123E-siRNAres-V5-SBP-MBP_S

    Plasmid

    #147471

    Purpose

    Mammalian Expression of HsSmg5-Q123E-siRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

    Insert

    HsSmg5-Q123E-siRNAres (SMG5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    three non silent mutation A254G (K85R), T980C (L3…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge