We narrowed to 806 results for: Sf1

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pGL2-Full

Plasmid

#16012

Depositor

Wafik El-Deiry

Article

Takimoto et al Oncogene. 2000 Mar 30. 19(14):1735-43.

Insert

KILLER/DR5 (TNFRSF10B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pBTK1024 (pDS-Def1)

Plasmid

#183125

Purpose

Expresses double-stranded RNA targeting the Def1 gene from Apis mellifera

Depositor

Jeffrey Barrick

Article

Lariviere et al Nat Protoc. 2023 Mar;18(3):902-928. doi:

Insert

Fragment of Def1 gene

Use

Synthetic Biology

Available Since

May 19, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pGL2_168

Plasmid

#199100

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

RSF1010 ORI plus NGS barcode 14, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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CMV566 A-USF

Plasmid

#33360

Depositor

Charles Vinson

Article

Qyang et al Mol Cell Biol. 1999 Feb;19(2):1508-17.

Insert

USF (Usf1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Dominant Negative (see notes below)

Promoter

CMV

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only
hTspan12(1-252)-6xHis-3C-sGFP11_pVL1393

Plasmid

#216382

Purpose

Baculovirus transfer vector to express human Tspan12 ∆C (residues 1-252) fused to split GFP s11

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Tetraspanin-12 (TSPAN12 Human)

Use

Baculovirus

Tags

6xHis-3C-splitGFP11

Expression

Insect

Promoter

Polyhedrin

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-NAD

Plasmid

#58345

Purpose

pEGFP-Mirt with a short intron from NDUFS1 cloned in

Depositor

Matthew Wood

Article

Seow et al RNA. 2012 Jul;18(7):1328-37. doi: 10.126

Insert

NDUSF1 intron 6 (NDUFS1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)

Plasmid

#101105

Purpose

Encodes dCas9(C) (without VP64) fused to VEGFR1

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR1-dCas9(C)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (+) NGFR-delta wt

Plasmid

#80020

Purpose

Mammalian expression of NGFR-minus cytoplasmic tail + KLGFFKR

Depositor

Chinten James Lim

Insert

NGFR (NGFR Human)

Tags

KLGFFKR

Expression

Mammalian

Mutation

minus cytoplasmic tail + KLGFFKR

Promoter

CMV

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
MBP-3C-hTspan12LEL(118-218)_pAcGP67a

Plasmid

#216383

Purpose

Baculovirus transfer vector to secrete human Tspan12 LEL (residues 118-218) fused to MBP

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Tetraspanin-12 (TSPAN12 Human)

Use

Baculovirus

Tags

GP64 signal sequence-MBP-3C

Expression

Insect

Promoter

Polyhedrin

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.425

Plasmid

#203944

Purpose

Up Flank. Up flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

Cas12a UP

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.426

Plasmid

#203945

Purpose

Down Flank. Down flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

Cas12a DOWN

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAC17V5a

Plasmid

#197413

Purpose

IPTG-inducible two-plasmid system bearing CP25 > lacO-GFP, specR, RSF1010 replicon (1/2)

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

lacO-GFP

Use

Synthetic Biology

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (+) NGFR-delta scr

Plasmid

#80021

Purpose

Mammalian expression of NGFR-KLRFGFK

Depositor

Chinten James Lim

Insert

NGFR (NGFR Human)

Tags

KLRFGFK

Expression

Mammalian

Mutation

fused to KLRFGFK

Promoter

CMV

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pKEK3238

Plasmid

#234092

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

sfCherry2

Expression

Bacterial

Mutation

E117Q/T127I/G219A

Promoter

pJ23100-BsaI

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pHSP02

Plasmid

#117259

Purpose

Genome editing for Lactobacillus plantarum WCSF1

Depositor

Sheng Yang

Article

Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

Insert

Cas9, promotor P11-guide-RNA, homologous arms of Lp_0537

Use

Other

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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PB-SAM-Dest

Plasmid

#102563

Purpose

Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.

Depositor

Ying Liu

Article

Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

Type

Empty backbone

Use

CRISPR; Piggybac transposon

Expression

Mammalian

Promoter

N/A

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-TRAIL

Plasmid

#10953

Depositor

Bingliang Fang

Article

Kagawa et al Cancer Res. 2001 Apr 15. 61(8):3330-8.

Insert

Tumor necrosis factor alpha related apoptosis inducing ligand (TNFSF10 Human)

Tags

GFP

Expression

Mammalian

Mutation

NA

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pBWB424

Plasmid

#140638

Purpose

RSF1010-Cas9-vanAB gRNA. Vector with constitutive Cas9 and gRNA targeting vanAB locus.

Depositor

Keith Tyo

Article

Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

Insert

Cas9

Expression

Bacterial

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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pGH020_sgRNA_G418-GFP

Plasmid

#85405

Purpose

hu6 driven sgRNA vector with G418 and GFP selectable markers

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

G418 resistance and GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBroad-T7RNAP-chlorR

Plasmid

#199992

Purpose

T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) chlorR

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

T7RNAP Circuit

Expression

Bacterial

Mutation

copFnull

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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