Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 6,501 results for: nls

Clear keywords
Showing: 461 - 480 of 6501 results

  1. LbCas12a-2C-NLS in pCSDest

    Plasmid
    #126638
    Purpose
    Expresses LbCas12a-2C-NLS in mammalian cell
    Depositor
    Scot Wolfe
    Publication
    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LbCas12a-2C-NLS
    Use
    CRISPR
    Tags
    3xHuman influenza hemagglutinin (HA)-Tag
    Expression
    Mammalian
    Promoter
    CMV IE94 promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126638-plasmid-map-sequence-id-247967

    Enlarge

  2. BPK2797

    Plasmid
    #72251
    Purpose
    Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335Q, and T1337R
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72251-plasmid-map-sequence-id-131872

    Enlarge

  4. pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1

    Plasmid
    #87828
    Purpose
    High-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.
    Depositor
    Andrea Musacchio
    Publication
    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    NLS
    PTS1
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Promoter
    CMV promoter, tetracycline operator
    Available since
    April 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    87828-plasmid-map-sequence-id-175012

    Enlarge

  12. pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS

    Plasmid
    #115694
    Purpose
    All-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.
    Depositor
    Erik Sontheimer
    Publication
    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    NmeCas9
    Nme-sgRNA
    Use
    CRISPR
    Tags
    NLS and NLS, HA
    Expression
    Insect and Mammalian
    Mutation
    None and fusion of repeat/tracrRNA to make a sgRNA
    Promoter
    EF-1 alpha and U6
    Available since
    Sept. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    115694-plasmid-map-sequence-id-221986

    Enlarge

  14. pcDNA-dCas9-p300 Core

    Plasmid
    #61357
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    55 Articles
    61357-plasmid-map-sequence-id-358013

    Enlarge

  15. pC015 - dLwCas13a-NF

    Plasmid
    #91905
    Purpose
    Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging
    Depositor
    Feng Zhang
    Publication
    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLwCas13a
    Use
    CRISPR
    Tags
    NLS and eGFP, ZF CCR5TC, KRAB-A, NLS
    Expression
    Mammalian
    Mutation
    R474A and R1046A
    Promoter
    CAG
    Available since
    Oct. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    91905-plasmid-map-sequence-id-198453

    Enlarge

  19. pCMV-BE-VQRCas9

    Plasmid
    #101740
    Purpose
    expression of an optimized Cas9 for base-editing in zebrafish
    Depositor
    Shuo Lin
    Publication
    Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, VQR mutations
    Promoter
    CMV
    Available since
    Jan. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    101740-plasmid-map-sequence-id-198315

    Enlarge

Showing: 461 - 480 of 6501 results