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Fuw-dCas9-Tet1CD-P2A-BFP

Plasmid

#108245

Purpose

Lentiviral vector to express dCas9-Tet1CD-P2A-tagBFP

Depositor

Rudolf Jaenisch

Publication

Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

Insert

dCas9-Tet1CD-HA-P2A-BFP

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

dCas9-D10A, dCas9-H840A

Promoter

Ubc

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pAAV-U6-gRNA-CBh-mCherry

Plasmid

#91947

Purpose

Expression of empty gRNA cassette and mCherry

Depositor

Jimok Kim

Publication

Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

Insert

mCherry

Use

AAV

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCA24N-ligase

Plasmid

#87741

Purpose

IPTG-inducible expression of T4 DNA ligase for protein purification

Depositor

Wayne Patrick

Publication

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis

Expression

Bacterial

Promoter

T5-lac

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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T7-VEE-IRES-Puro

Plasmid

#58970

Purpose

noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Publication

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Type

Empty backbone

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

26S subgenomic promoter

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pL-CRISPR.SFFV.GFP

Plasmid

#57827

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Publication

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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AAVS1-CAG-hrGFP

Plasmid

#52344

Purpose

AAVS1 donor vector for constitutive gene expression

Depositor

Su-Chun Zhang

Publication

Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hrGFP

Expression

Mammalian

Promoter

CAG

Available since

July 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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pCMV-Bx

Plasmid

#51552

Purpose

Expresses Bxb1 integrase in mammalian cells.

Depositor

Michele Calos

Publication

Zhu et al Nucleic Acids Res. 2013 Dec 4.

Insert

Bxb1 integrase

Expression

Mammalian

Promoter

CMV

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pST1374-N-NLS-flag-linker-Cas9-D10A

Plasmid

#51130

Purpose

Cas9 nickase optimized for nuclear import.

Depositor

Xingxu Huang

Publication

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9 nickase

Use

CRISPR

Tags

Flag and NLS

Expression

Mammalian

Mutation

D10A (nickase mutant)

Promoter

CMV

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pcDNA3-Drp1K38A

Plasmid

#45161

Depositor

Alexander van der Bliek, Richard Youle

Publication

Smirnova et al J Cell Biol. 1998 Oct 19;143(2):351-8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Drp1 (DNM1L Human)

Tags

HA (AYPYDVPDY)

Expression

Mammalian

Mutation

K38A

Promoter

CMV

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Lenti-Hb9-GFP

Plasmid

#37080

Purpose

3rd generation lentiviral transfer plasmid for Hb9 GFP

Depositor

Fred Gage

Publication

Marchetto et al Cell Stem Cell. 2008 Dec 4;3(6):649-57.

Insert

Hb9 GFP

Use

Lentiviral

Promoter

Hb9

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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