We narrowed to 9,856 results for: UTY

1. pLCKO_PSMB2_sgRNA_1

   Plasmid

   #74184

   Purpose

   lentiviral vector expressing sgRNA targeting PSMB2

   Depositor

   Jason Moffat

   Article

   Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

   Insert

   PSMB2 sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 Promoter

   Available Since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-Tac-TC

   Plasmid

   #162492

   Purpose

   Expresses partial length Tac (TMD and cytosolic tail) with GFP tag in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

   Insert

   partial length Tac (TMD and cytosolic tail) (IL2RA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Tac is in partial length with its TMD and cysotol…

   Promoter

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. FusionRed-Dectin1A-N-10

   Plasmid

   #56112

   Purpose

   Localization: Membrane, Excitation: 580, Emission: 608

   Depositor

   Michael Davidson

   Article

   Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

   Insert

   Dectin1A (CLEC7A Human)
   

   Use

   Tags

   FusionRed

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pSMART-ala10

   Plasmid

   #87135

   Purpose

   NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Inserts

   NADPH Alanine Dehydrogenase (ald Synthetic)
   alanine exporter (alaE Synthetic)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D196A, L197R

   Promoter

   waaHp from E. coli as part of operon with ald* an…

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-2TK cyclin T1 (1-551) (P#422)

   Plasmid

   #14609

   Depositor

   Matija Peterlin

   Article

   Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

   Insert

   cyclin T1 (1-551) (CCNT1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 1-551

   Promoter

   Available Since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

6. L309-myc-seipin-ΔCT

   Plasmid

   #64062

   Purpose

   myc-tagged murine seipin with deletion of cytosolic C-terminus); lentiviral construct

   Depositor

   Weiping Han

   Article

   Yang et al Biochem J. 2013 May 15;452(1):37-44. doi

   Insert

   bscl2 (Bscl2 Mouse)
   

   Use

   Lentiviral

   Tags

   myc epitope

   Expression

   Mammalian

   Mutation

   Deletion of C-terminal Asp338–Ser443

   Promoter

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pMP71-tCD19-llo-mc38tmg

   Plasmid

   #174603

   Purpose

   expresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the LLO190 and minigenes of 3 neoantigens from the MC38 tumor in genes ADGPK, DPAGT and Reps1

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   extracell transmem domains moCD19 wCterm fusion LLO190 and minigenes of 3neoantigens MC38tumor in genes ADGPK DPAGT Reps1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. B9L-BirA*

   Plasmid

   #109146

   Purpose

   To generate stable cell lines expressing B9L-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells

   Depositor

   Mariann Bienz

   Article

   van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife

   Insert

   B9L (Bcl9l Mouse)
   

   Use

   Tags

   BirA(R118G) and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO2

   Available Since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV2-U6gRNA5(Vps39-g1)-PGKpuroBFP-W

   Plasmid

   #105019

   Purpose

   Lentiviral gRNA plasmid targeting mouse Vps39 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Vps39 (Vps39 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG69 G6PD 3'UTR mut

    Plasmid

    #12053

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    G6PD 3'UTR mut (G6PD Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Promoter

    Available Since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFUSEss-CHIg-mG3_Lys322Arg

    Plasmid

    #105862

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Lys322Arg (EU numbering).

    Depositor

    Joanna Bereta

    Article

    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

    Insert

    IgG3 heavy chain (Ighg3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lys322Arg in constant region of IgG1 heavy chain

    Promoter

    hEF1-HTLV prom

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Hy_pMT Flag-UAP56 MUT

    Plasmid

    #110124

    Purpose

    Expresses Flag-tagged human UAP56 (DDX39B) with 4 mutations that impact circRNA nuclear export activity

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    UAP56 (DDX39B Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    4 mutations (KSLN motif changed to RSFS)

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. ADAP1

    Plasmid

    #163905

    Purpose

    Full length mouse ADAP1 for purification from insect cells

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    ADAP1 (Adap1 Mouse)
    

    Use

    Tags

    Cleavable 6 x HIS tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. p28BIOHTEV-LIC: nsp16_SARS-CoV-2

    Plasmid

    #167846

    Purpose

    Bacterial expression of the SARS-CoV-2 protein nsp16

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp16
    

    Use

    Tags

    6xHis and AviTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pAG150 FGFR1 3'UTR

    Plasmid

    #12008

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    N15 3'UTR (FGFR1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNAmyc-hPR-B-486-933

    Plasmid

    #89118

    Purpose

    mammalian expression of human progesterone receptor amino acid residues 486-933 with N-terminal myc tag, has PR DNA and ligand binding domains

    Depositor

    Elizabeth Wilson

    Article

    Liu et al J Biol Chem. 2011 Aug 26;286(34):29951-6

    Insert

    human progesterone receptor ligand binding domain (PGR Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    contains aa 486-933

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLCKO_PSMB2_sgRNA_5

    Plasmid

    #74185

    Purpose

    lentiviral vector expressing sgRNA targeting PSMB2

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    PSMB2 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEF Doc2beta 6X mutant GFP

    Plasmid

    #128823

    Purpose

    To visualize Doc2beta 6X mutant in PC12 cells

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2beta (Doc2b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D163A, D218A, D220N, D303A, D357A, D359A

    Promoter

    EF-1alpha

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. G protein alpha-o-R179C-EE

    Plasmid

    #55780

    Purpose

    This is a constitutively activated G protein alpha-o mutant containing an internal EE epitope.

    Depositor

    Catherine Berlot

    Article

    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

    Insert

    G protein alpha-o with constitutively activating R179C mutation (Gnao1 Rat)
    

    Use

    Tags

    EE epitope was introduced internally with D167E a…

    Expression

    Bacterial and Mammalian

    Mutation

    Arginine 179 was replaced with cysteine

    Promoter

    CMV

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pACYC-mTET1-N

    Plasmid

    #81056

    Purpose

    bacterial expression of N-terminus of murine TET1

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TET1 (Tet1 Mouse)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    N-terminus, amino acids 301-1366

    Promoter

    T7

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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