We narrowed to 26,741 results for: STI

1. evoCas9

   Plasmid

   #138558

   Purpose

   Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   evoCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   M495V, Y515N, K526E, R661Q

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCK354

   Plasmid

   #129698

   Purpose

   For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120010799

   Depositor

   John Heap

   Article

   Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

   Inserts

   PrhaBAD
   yfp
   rhaS
   Terminator ECK120010799
   

   Expression

   Bacterial

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. IKK2-EYFP

   Plasmid

   #111196

   Purpose

   fluorescent fusion protein

   Depositor

   Johannes A. Schmid

   Article

   Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

   Insert

   IKKbeta (IKBKB Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. KRAS2B

   Plasmid

   #55655

   Purpose

   expression of mouse KRAS2B

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   KRAS2B (Kras Mouse)
   

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pAAV hSyn1 ChR2 ET-TC 2A tDimer

   Plasmid

   #101361

   Purpose

   High efficiency channelrhodopsin, neuron specific promoter, red fluorescent protein

   Depositor

   Thomas Oertner

   Article

   Berndt et al Proc Natl Acad Sci U S A. 2011 May 3;108

   Inserts

   Channelrhodopsin-2
   synaptophysin-red fluorescent protein
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   E123T , T159C

   Promoter

   human Synapsin

   Available Since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAf-CRISPR-phoA

   Plasmid

   #207991

   Purpose

   CRISPR vector used with pAf-CRISPR-ctfR1 (#207992) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavus

   Depositor

   Perng-Kuang Chang

   Article

   Chang et al Fungal Genet Biol. 2023 Dec 27;170:10386

   Insert

   phoA
   

   Use

   CRISPR

   Promoter

   Aspergillus flavus U6

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-hPGK-mCherry-SynaptoZip

   Plasmid

   #177317

   Purpose

   Lentiviral expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by hPGK promoter

   Depositor

   Antonio Malgaroli

   Article

   Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

   Insert

   mCherry-SynaptoZip (Vamp2 Rat)
   

   Use

   Lentiviral

   Tags

   Myc and mCherry

   Expression

   Mammalian

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCI.neo-rPAM1-M314I

   Plasmid

   #145802

   Purpose

   expresses catalytically inactive rat PAM-1 in mammalian cells

   Depositor

   Richard Mains, Betty Eipper

   Article

   Eipper et al Biochemistry. 1995 Mar 7;34(9):2857-65.

   Insert

   rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
   

   Expression

   Mammalian

   Mutation

   changed methionine 314 to isoleucine to inactivat…

   Available Since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV_12xTEAD_luc2

   Plasmid

   #173004

   Purpose

   firefly luciferase reporter vector with 12x clustered TEAD binding sites

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   12xTEAD-luc2
   

   Use

   AAV and Luciferase

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-puro GFP-UVRAG

    Plasmid

    #38267

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

    Insert

    UV radiation resistance-associated gene protein (UVRAG Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pDONR P4-P1R-mKate2

    Plasmid

    #48344

    Purpose

    Contributes the coding sequence for the mKate2 fluorescent protein as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Tomas Santalucia, Anna Planas

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    mKate2
    

    Use

    Gateway entry vector

    Mutation

    It contains a Kozak sequence but no stop codon.

    Promoter

    none

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. eSpCas9-EF-Gsk3b(new)

    Plasmid

    #122341

    Purpose

    Expresses sgRNA targeting mouse Gsk3b and eSpCas9 in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Gsk3b
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTol1-UAS:Chronos-tdTomato

    Plasmid

    #124232

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin Chronos fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    Chronos-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Promoter

    14xUAS-E1b

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMM9-MAPKK2-KW71 [delta(48-55)/S222D/S226D]

    Plasmid

    #40806

    Depositor

    Natalie Ahn

    Article

    Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

    Insert

    MAP2K2 (MAP2K2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta(48-55) + S222D + S226D

    Promoter

    MSV-LTR

    Available Since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3 merlin 1-590

    Plasmid

    #11624

    Depositor

    Vijaya Ramesh

    Article

    Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

    Insert

    merlin (NF2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 3, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pMSCV tubulin myc

    Plasmid

    #12581

    Depositor

    David Sabatini

    Article

    Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

    Insert

    tubulin (TUBG1 Human)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    *

    Available Since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1-blast shGAC

    Plasmid

    #110410

    Purpose

    shGAC (Target CCTCTGTTCTGTCAGAGTT), silence glutaminase isoform GAC, blasticidin selection.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GLS glutaminase (GLS Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6 (RNA Pol III)

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXs_FLAG-SFXN5

    Plasmid

    #110639

    Purpose

    Retroviral expression of Flag-SFXN5 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    SFXN5 (SFXN5 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a(DAP10)

    Plasmid

    #177904

    Purpose

    Bacterial expression of his10-tagged human DAP10 Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    DAP10 (aa 70-92 only) (HCST Human)
    

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. px330 RBP1 gRNA

    Plasmid

    #165593

    Purpose

    Insertion of RBP1 degron

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    RBP1 (Polr2a Mouse)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. VQR variant

    Plasmid

    #138560

    Purpose

    Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VQR
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    D1135V, R1335Q, T1337R

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pclbw-PAGFPomp25

    Plasmid

    #58427

    Purpose

    Mammalian expression of photoactivatable GFP targeted to the mitochondrial outer membrane

    Depositor

    David Chan

    Article

    Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

    Insert

    paGFP-omp25 (Synj2bp Mouse)
    

    Use

    Retroviral

    Tags

    PAGFP

    Expression

    Mammalian

    Mutation

    contains aa's 109-145 of mouse omp25

    Promoter

    cmv-bactin

    Available Since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

23. Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37b, 46]

    Plasmid

    #26570

    Depositor

    Diane Lipscombe

    Article

    Lin et al Neuron. 1997 Jan . 18(1):153-66.

    Insert

    calcium channel alpha-1B subunit (Cacna1b Rat)
    

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Deletion of exons a10, 18a, and 24a. Contains …

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

24. pACEBac1-beta-actin

    Plasmid

    #178076

    Purpose

    Insect cell expression of beta-actin

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Insert

    beta-actin (ACTB Human)
    

    Expression

    Insect

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pEGFP-C1-KSENB-Nup133-mut

    Plasmid

    #87339

    Purpose

    To express human Nup133 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP133 (NUP133 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes; deletion of Nup133 AA1-7

    Promoter

    CMV

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. pUAST-SIK3 T196E

    Plasmid

    #52975

    Purpose

    insect expression of SIK3 T196E constitutively active mutant

    Depositor

    Marc Montminy, Biao Wang

    Article

    Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

    Insert

    SIK3 (Sik3 Fly)
    

    Tags

    HA

    Expression

    Insect

    Mutation

    T196E constitutively active mutant

    Promoter

    Hsp70

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pMY-MCS-P2A-BlastR-DYK

    Plasmid

    #163357

    Purpose

    Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated Blasticidin selection marker

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCD86-mApple

    Plasmid

    #133859

    Purpose

    Mammalian expression of CD86 C-terminally tagged with mApple

    Depositor

    Dave Piston

    Article

    Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

    Insert

    CD86 (CD86 Human)
    

    Tags

    mApple

    Expression

    Mammalian

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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29. pHisAlix deltaPRD

    Plasmid

    #42577

    Depositor

    James Hurley

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

    Insert

    delta-PRD ALIX (residues 1-702) (PDCD6IP Human)
    

    Tags

    HIS-TEV

    Expression

    Bacterial

    Mutation

    silent mutation A1617G (numbering in ALIX gene)

    Available Since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pPbuCas13b-gRNA-NT

    Plasmid

    #184568

    Purpose

    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PbuCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Constitutive expression of single-spacer CRISPR array witha nontargeting spacer for PbuCas13b in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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31. pXPA-fapO-RgTALsyn

    Plasmid

    #100946

    Purpose

    Expression of Tyrosine Ammonia Lyase from constitutive GAP promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Insert

    RgTALsyn
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    GAP (Constitutive)

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3 PBD HK-AA (Nigg AH13)

    Plasmid

    #41161

    Depositor

    Erich Nigg

    Article

    Hanisch et al Mol Biol Cell. 2006 Jan;17(1):448-59. Ep

    Insert

    PBD HK-AA (PLK1 Human)
    

    Tags

    3xMyc and PreScission Site

    Expression

    Mammalian

    Mutation

    Contains Plk1-PBD only (aa 326–603); HK-AA (H538A…

    Promoter

    CMV

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

33. pcDNA5 HA-Arp8

    Plasmid

    #19796

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

    Insert

    Arp8 (ACTR8 Human)
    

    Tags

    2XHA

    Expression

    Mammalian

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

34. pSHS325 - Bacterial expression plasmid for SpCas9 REC3 domain

    Plasmid

    #101205

    Purpose

    Bacterial expression plasmid for SpCas9 REC3 domain

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K506–Q712
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K506–Q712

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. RnBarr1-mTurquoise2

    Plasmid

    #137789

    Purpose

    Visualization of beta-arrestin1

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Rat beta-arrestin 1 (Arrb1 Rat)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSG5-Myr-FLAG-p110α-DM

    Plasmid

    #55713

    Purpose

    eukaryotic expression of myristoylation tag (Myr-FLAG) p110a RBD double mutant T207D, K227A

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    p110 alpha (Pik3ca Mouse)
    

    Tags

    myristoylation tag (Myr-FLAG)

    Expression

    Mammalian

    Mutation

    T207D, K227A

    Promoter

    T7

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

37. pcDNA3 merlin 1-332

    Plasmid

    #11625

    Depositor

    Vijaya Ramesh

    Article

    Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

    Insert

    merlin (NF2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Amino-terminal half of merlin.

    Available Since

    May 3, 2006

    Availability

    Academic Institutions and Nonprofits only

38. pKM60

    Plasmid

    #154159

    Purpose

    Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Mutation

    4 wt NF-κB sites were replaced with Rel1A-specifi…

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFlox_N-Mau2_BSD_FKBPF36V_EYFP

    Plasmid

    #163880

    Purpose

    Acute Depletion of Mau2-EYFP

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    Mau2 (Mau2 Mouse)
    

    Expression

    Mammalian

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLNCX2-Orai1

    Plasmid

    #89818

    Purpose

    Express Orai1 in mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    Orai1 (ORAI1 Human)
    

    Use

    Retroviral

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge