162,590 results

1. AAVS1-Pur-CAG-hM4Di-mCherry

   Plasmid

   #80947

   Purpose

   human AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry expression cassette driven by CAG promoter

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

   Insert

   hM4Di-mCherry
   

   Use

   Tags

   mcherry fusion

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-hDcp2

   Plasmid

   #72214

   Purpose

   bacterial expression of His-tagged human Dcp2

   Depositor

   Megerditch Kiledjian

   Article

   Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

   Insert

   Dcp2 (DCP2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA-Ikkb-FLAG(K44A)

   Plasmid

   #23299

   Depositor

   Warner Greene

   Article

   Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

   Insert

   Ikkbeta (IKBKB Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K44A

   Promoter

   Available since

   April 16, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pT7-EGFP-C1-HsEDC4_S

   Plasmid

   #147455

   Purpose

   Mammalian Expression of HsEDC4. Please note that this plasmid does not contain the T7 promoter.

   Depositor

   Elisa Izaurralde

   Article

   Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

   Insert

   HsEDC4 (EDC4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDF-5xT7-TtCsm

   Plasmid

   #128572

   Purpose

   For expression of T. thermophilus Csm in E. coli (Csm1-5)

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

   Inserts

   Csm1
   Csm2, Csm3, Csm4, Csm5
   

   Use

   Tags

   HRV 3C protease cleavage site and 10xHis tag on C…

   Expression

   Bacterial

   Mutation

   Codon optimized for expression in E. coli and Cod…

   Promoter

   T7 promoter, lac operator

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA 3.1 myc-Rab10

   Plasmid

   #208366

   Purpose

   Mammalian expression of myc-tagged human Rab10

   Depositor

   Suzanne Pfeffer

   Article

   Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

   Insert

   myc-Rab10 (RAB10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   Available since

   Dec. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. F5-bio-His

   Plasmid

   #53426

   Purpose

   Expresses full-length Coagulation factor V precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   F5 (F5 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. 2xmyc-RAB10

   Plasmid

   #164631

   Purpose

   Expresses RAB10 in mammalian cells with a 2xmyc tag

   Depositor

   Mark Cookson

   Article

   Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

   Insert

   Ras-related protein Rab-10 (RAB10 Human)
   

   Use

   Tags

   2xmyc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AICSDP-116:PCNA-mEGFP

   Plasmid

   #170818

   Purpose

   Homology arms and mEGFP-linker sequence for N-terminus tagging of human PCNA

   Depositor

   Allen Institute for Cell Science

   Insert

   PCNA Homology Arms with mEGFP-linker (PCNA Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   June 11, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pNIFV

    Plasmid

    #111085

    Purpose

    pNIFV is a vector plasmid with mutations in the cis-acting region, to prevent correction of the integrase by recombination when used with pCiPS-NIFV to produce Non-Integrating Foamy Virus vectors

    Depositor

    David Russell

    Article

    Deyle et al J Virol. 2010 Sep;84(18):9341-9. doi: 10

    Insert

    empty backbone/deleted foamy vector, same as pΔΦ with mutations in cis-acting pol region
    

    Use

    Deleted foamy virus

    Tags

    Expression

    Mutation

    at nt 2334 A changed to T, at nt 2495 A changed t…

    Promoter

    CMV

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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