We narrowed to 9,314 results for: Coli

1. pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

   Plasmid

   #176534

   Purpose

   Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   CRISPR-associated IscB (Delaware Bay metagenome)
   

   Use

   Tags

   14xHis-MBP-bdSUMO and TwinStrep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSFDuet-1_VNp-LZ_VNp-LZ-hGH

   Plasmid

   #182412

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine zipper-human growth hormone fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Inserts

   VNp-LZ-hGH (GH1 Human)
   VNp-LZ
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3'-ecRESCUE-mCherry

   Plasmid

   #191486

   Purpose

   ecDHFR DD-mCherry, an inducible ecRESCUE RNA base editor: TMP-induced ecDHFR DD expression allows reversible control of the expression of dRanCas13b-ADAR2-mCherry

   Depositor

   Xu Ma

   Article

   Wang et al Cell Commun Signal. 2021 Jul 31;19(1):81

   Insert

   dRanCas13b-hADAR2-mCherry-ecDHFR (folA, ADARB1 Human, R. anatipestifer, E. coli)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-hGH

   Plasmid

   #182425

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-human growth hormone fusion proteins

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Inserts

   VNp-LZ-mNeongreen-hGH (GH1 Human)
   VNp-LZ
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp) and Vesicle Nucl…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. SPACE-VRQR (pRZ5137)

   Plasmid

   #140245

   Purpose

   CMV promoter expression plasmid for TadA*(V82G)-nCas9_VRQR-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   SPACE_VRQR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V82G in TadA*, VRQR mutations in SpCas9, D10A in …

   Promoter

   CMV

   Available since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNSSCP

   Plasmid

   #183188

   Purpose

   PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under T

   Depositor

   Hsiao-Chun Huang

   Article

   Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1

   Inserts

   DivIVA and superfolder GFP fusion protein
   SpmX450 and T7 181+ fusion protein
   T7 RNAP 1-179 variant and SpmX450 fusion protein
   mRFP1 and popZ fusion protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9

   Plasmid

   #136660

   Purpose

   Expresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNA

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9
   sgRNA for St1Cas9
   ABE7.10
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS and hSt1Cas9

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pdCas9-M-G6

   Plasmid

   #73437

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor G6 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   March 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pdCas9-M-3A2

   Plasmid

   #73435

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 3A2 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   March 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pdCas9-M-4A6

    Plasmid

    #73434

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4A6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 4A6 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCIBN-LacI-tagRFP-T

    Plasmid

    #103810

    Purpose

    'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-LacI-tagRFP-T (CIB1, lacI E. coli, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    LacI: C19T (silent, L7L), T264C (silent, A88A), C…

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAGE2.0

    Plasmid

    #165984

    Purpose

    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Tet resistance.

    Depositor

    Bogumil Karas

    Article

    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

    Inserts

    RK2/RP4 origin of transfer
    repA2B2C2
    nourseothricin N-acetyl transferase
    tetracycline resistance
    

    Use

    Synthetic Biology; Bacterial and yeast cloning; c…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-Etanercept

    Plasmid

    #182424

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-Etanercept fusion proteins

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-LZ-mNeongreen-Etanercept (TNFRSF1B Human)
    VNp-LZ
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp), and Vesicle Nuc…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLY305

    Plasmid

    #184159

    Purpose

    Reporter circuit for hijacking mRNA of zraP as CRISPR RNA

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    mutsfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    K140N, Mutations D10A and H840A on WT cas9. Synon…

    Promoter

    Pcon, PpspA-zraP-22, PrhaB, and Ptet

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR

    Plasmid

    #170808

    Purpose

    PiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistance

    Depositor

    Kenneth Yamada

    Article

    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

    Inserts

    GID1-VPR-IRES-HygR
    GAI-tagBFP-Sa_dCas9 (GAI Mustard Weed, Synthetic, S. aureus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Synonymous mutation in PYL1 to remove XhoI site (…

    Promoter

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pdCas9-M-C4

    Plasmid

    #73427

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant C4.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor C4 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. HisGB1-Hif1a(776-826)_FIH

    Plasmid

    #99508

    Purpose

    Bacterial expression of hydroxylated Hif1a TAD (776-826)

    Depositor

    Peter Wright

    Article

    Sugase et al Protein Expr Purif. 2008 Feb;57(2):108-1

    Inserts

    Hif1a (776-826) (HIF1A Human)
    FIH1 (HIF1AN Human)
    

    Use

    Tags

    His6GB1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDF-casBCDE(g8)

    Plasmid

    #89727

    Purpose

    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing 33-nt spacer (referred to as 'wt') matching a protospacer in the M13 phage genome.

    Depositor

    Konstantin Kuznedelov, Konstantin Severinov

    Article

    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

    Insert

    CRISPR array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-pro-siRNA-V2-LMNA

    Plasmid

    #111088

    Purpose

    pET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human Lamin A/C gene.

    Depositor

    Linfeng HUANG

    Article

    Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

    Insert

    Lamin A/C (LMNA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. WT MetNIQ in pBAD

    Plasmid

    #118253

    Purpose

    Wild Type Methionine Binding Protein and Methionine ABC Transporter in pBAD Vector for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (metN E. coli)
    MetI (metI E. coli)
    MetQ (metQ E. coli)
    

    Use

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 295 to Alanine (Ala)

    Promoter

    None and araBAD Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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