We narrowed to 13,035 results for: Mpl

1. pHis2-Arf1 (17-181)-Q71L

   Plasmid

   #184808

   Purpose

   Bacterial expression of Arf1 (aa17-181, Q71L mutation)

   Depositor

   James Hurley

   Article

   Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10

   Insert

   Arf1 (ARF1 Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   aa 17-181 only, Q71L

   Promoter

   T7

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET16b-Trx CC32/35SS

   Plasmid

   #36946

   Depositor

   Anna Blom

   Article

   King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

   Insert

   Thioredoxin 1 (TXN Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   both active site cysteines (32,35) mutated to ser…

   Promoter

   T7

   Available since

   May 20, 2013

   Availability

   Academic Institutions and Nonprofits only

3. PCMV-intron myc Rab6WT

   Plasmid

   #46781

   Purpose

   expression of WT Rab6a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   Rab6 (RAB6A Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTU424

   Plasmid

   #178983

   Purpose

   pACYC containing Candidatus “Scalindua brodae” TPR-CHAT (codon-optimized)

   Depositor

   Stan Brouns

   Article

   van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

   Insert

   TPR-CHAT
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAdEasy Flag GCN5

   Plasmid

   #14106

   Depositor

   Pere Puigserver

   Article

   Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

   Insert

   GCN5 (KAT2A Human)
   

   Use

   Adenoviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   E189G and K195N

   Promoter

   Available since

   Feb. 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS95 pHAGE2 CMVtetO2 GFP-22xGS-TEV-MCS

   Plasmid

   #199348

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-TEV-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP-TEV

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pscALPS-HIV-1 Group O-vpr

   Plasmid

   #115836

   Purpose

   Encodes codon optimized 3xFLAG-HIV1 Group O Vpr (L20571) and puromycin resistance protein

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   HIV1 Group O Vpr (L20571)
   

   Use

   Lentiviral

   Tags

   3x-FLAG

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRRL CAG NFlag BAF170 ires GFP

   Plasmid

   #24562

   Depositor

   Jerry Crabtree

   Article

   Ho et al Proc Natl Acad Sci U S A. 2009 Mar 31. 1

   Insert

   BAF170 (Smarcc2 Mouse)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pBB75-ncRNA

   Plasmid

   #194087

   Purpose

   Expresses Ec86 ncRNA in Escherichia coli

   Depositor

   Tingting Zou

   Article

   Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

   Insert

   Ec86 ncRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAGM9037

    Plasmid

    #153225

    Purpose

    Template for PCR amplification of the constant part of guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Insert

    guide RNA constant part, carotenoid marker
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-eCas9-sgMIB1

    Plasmid

    #140239

    Purpose

    Lentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Insert

    sgRNA targeting human MIB1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLC-255

    Plasmid

    #124292

    Purpose

    Expression of vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.

    Depositor

    Swaine Chen

    Article

    Eshaghi et al Angew Chem Int Ed Engl. 2015 Nov 16;54(4

    Insert

    vsfGFP-9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-3XFLAG-renilla-luciferase-beta-globin-(39PTC)

    Plasmid

    #184394

    Purpose

    Transient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein with PTC in beta-globin

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    Renilla luciferase
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJZC43

    Plasmid

    #66565

    Purpose

    sgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 2XPP7
    PCP-VP64 IRES mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. myc-hAtg13

    Plasmid

    #31965

    Depositor

    Do-Hyung Kim

    Article

    Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

    Insert

    Atg13 (ATG13 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pEGFP-C1-KSENB-Nup133

    Plasmid

    #87340

    Purpose

    To express human Nup133 in mammalian cells

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP133 (NUP133 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deletion of Nup133 AA1-7

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLENTI_RPS3

    Plasmid

    #127128

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS3 (RPS3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

    Plasmid

    #159849

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-ATXN1-30Q

    Plasmid

    #33238

    Depositor

    Huda Zoghbi

    Article

    Cummings et al Nat Genet. 1998 Jun;19(2):148-54.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 32 amino acids (30 Q…

    Promoter

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9

    Plasmid

    #84246

    Purpose

    Expresses direct fusion VPR-Sa dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    VPR-tagBFP-Sa dCas9
    Puro
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Nucleoplasmin NLS, SV40 NLS, VPR, and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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