We narrowed to 7,181 results for: RAP

1. pUTKan-3xHA-GFP-TOM5

   Plasmid

   #130674

   Purpose

   Ubiquitously expresses 3xHA-GFP-TOM5 in the outer mitochondrial membrane of planta

   Depositor

   Andreas Weber

   Article

   Kuhnert et al Plant Physiol. 2020 Feb;182(2):692-706.

   Insert

   Translocase of outer mitochondrial membrane 5 (TOM5 Mustard Weed)
   

   Use

   Tags

   3xHA and sGFP

   Expression

   Plant

   Mutation

   Promoter

   Ubiquitin10

   Available since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. AA008

   Plasmid

   #216003

   Purpose

   Fragmid fragment: (Cas protein) preferred over wildtype Cas12a

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas12a_v1.1 [EnAs]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-SNAP-CRY2-VHH(GFP)

   Plasmid

   #58370

   Purpose

   For use in light-induced inactivation of GFP fused protein through the interaction with CIB1-MP

   Depositor

   Won Do Heo

   Article

   Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

   Insert

   CRY2[1-498] (CRY2 Mustard Weed)
   

   Use

   Tags

   SNAP and vhhGFP4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. mKate2-P2A-APEX2-ML1N*2

   Plasmid

   #67665

   Purpose

   Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain, [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   mKate2-P2A-APEX2-ML1N*2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMK283 (Stag-3FLAG-Neo)

   Plasmid

   #72799

   Purpose

   C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   Stag-3FLAG-Neo
   

   Use

   CRISPR

   Tags

   Stag-3FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG/hTAZ-FL

   Plasmid

   #139979

   Purpose

   Expresses full length human Tafazzin with codon modified for cloning. Transgene flanked by AAV2 ITR

   Depositor

   William Pu

   Article

   Wang et al Circ Res. 2020 Mar 9. doi: 10.1161/CIRCR

   Insert

   Full length human Tafazzin (TAFAZZIN Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TfR-mCh-DHFR

   Plasmid

   #173013

   Purpose

   For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter.

   Depositor

   Matthew Kennedy

   Article

   Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   TfR-mCh-DHFR
   

   Use

   Tags

   DHFR and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAR-B1(1986.1)

   Plasmid

   #78503

   Purpose

   Trans plasmid for production of recombinant AAV-B1 vectors. Plasmid encodes AAV2 Rep and AAV-B1 capsid

   Depositor

   Miguel Sena-Esteves

   Article

   Choudhury et al Mol Ther. 2016 Apr 27. doi: 10.1038/mt.2

   Inserts

   AAV2 Rep
   AAV-B1 capsid
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. hP2X3-cALFA

   Plasmid

   #160499

   Purpose

   For the expression of human P2X3 with ALFA tag at C-terminus in mammalian cells

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Insert

   human P2X3 (P2RX3 Synthetic, Human)
   

   Use

   Tags

   HRV3C protease cleavage site-ALFA tag-twin Strep …

   Expression

   Mammalian

   Mutation

   ΔN5ΔC33-T13P/S15V/V16I

   Promoter

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTS114 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-Alfa-P2A-TagBFP

    Plasmid

    #199367

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TEV-SUMOEu-ALFA-P2A-TagBFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX Flag RagC W115R

    Plasmid

    #112759

    Purpose

    express Flag-RagC (W115R mutant)

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-RagC (W115R) (RRAGC Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    W115R

    Promoter

    Available since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLyGo-Ec-7

    Plasmid

    #163135

    Purpose

    pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the C-IgAP surface expression anchor, nanobody, and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Nanobody, C-IgAP autotransporter

    Expression

    Bacterial

    Mutation

    Promoter

    P(rhaB)

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX Flag RagC T90N

    Plasmid

    #112758

    Purpose

    express Flag-RagC (T90N mutant)

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-RagC (T90N) (RRAGC Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    T90N

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEMHE-mCherry-mTrim21ΔRING-Box

    Plasmid

    #105523

    Purpose

    T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Use

    In vitro transcription

    Tags

    mCherry

    Expression

    Mutation

    deletion of RING domain (aa124-462)

    Promoter

    T7

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. YF-Cdc42(CA)

    Plasmid

    #20152

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    YFP-FKBP-Cdc42(CA) (CDC42 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cdc42H was inserted into the multiple cloning sit…

    Promoter

    Available since

    March 26, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Syn-pAceR

    Plasmid

    #195521

    Purpose

    Red fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    pAceR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    VARNAM 78E, 81D, 92N; SI linker

    Promoter

    Synapsin

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. CoV2-N-R203K

    Plasmid

    #177951

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203K). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (R203K) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R203K

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NELC492A-mcherry-Tnos

    Plasmid

    #211845

    Purpose

    Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase, where the ligase is catalytically inactive.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Inserts

    LRR-NEL
    GAL4-VP16-GR
    

    Use

    Synthetic Biology

    Tags

    HA, Myc, and mCherry

    Expression

    Plant

    Mutation

    C492A

    Promoter

    6xUAS-35Smini and pUBQ10

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBMN mCherry-NDP52(C443K)

    Plasmid

    #119685

    Purpose

    For mammalian expression of UBD mutant NDP52

    Depositor

    Michael Lazarou

    Article

    Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    C443K

    Promoter

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBEG R2-dsRed-L3 #BGV203

    Plasmid

    #48973

    Purpose

    Supplies the selection marker (dsRed) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Insert

    dsRed
    

    Use

    Gateway cloning vector

    Tags

    IRES (strong)

    Expression

    Mutation

    Promoter

    none

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only