We narrowed to 36,578 results for: kan
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Plasmid#112255PurposeExpresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccinationDepositorInsertSpyCatcher-mi3
TagsC-tagExpressionBacterialPromoterT7Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJL1-dTomato
Plasmid#102631PurposeIn vitro expression of tdTomato from the T7 promoterDepositorInserttdTomato
ExpressionBacterialPromoterT7Available SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
hKv2.1-pEGFP-C1
Plasmid#111538PurposeExpresses human KCNB1 in mammalian cells for imagingDepositorAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab13
Plasmid#110500PurposeFluorescent Rab13DepositorAvailable SinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-TEV-KRAS G13D
Plasmid#111851PurposeExpresses activated human KRAS G13D (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS G13D (KRAS Human)
Tags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationChanged Glycine 13 to Aspartic AcidPromoterT5Available SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmKate2-MS2coatProtein
Plasmid#103833PurposeLive cell reader for MS2 loop RNA productionDepositorInsertmKate2-MS2 coat protein (cp )
ExpressionMammalianMutationMCP: nt 202-240 are missing (13 aa), C242G (A81G)…PromoterCMVAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB2-2ndVal mScarlet-I
Plasmid#104028PurposePlasmid encoding 2ndVal mScarlet-IDepositorInsert2ndVal mScarlet-I
UseLow copyExpressionBacterialMutationmScarlet-I with valine inserted in 2nd positionPromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin
Plasmid#99277PurposeDual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistanceDepositorInsertsH2B-mOrange2-IRES-EGFP
Puro
UseLentiviralTagsEGFP, H2B, IRES, PGK, and mOrange2PromoterEF1 alpha-human and PGK-mouseAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_21A
Plasmid#91128PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:hpt IIDepositorInsertEngineering Reagent: 35S:AtCas9 + AtU6:gRNA
UseCRISPRExpressionPlantAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-KSENB-Nup133
Plasmid#87340PurposeTo express human Nup133 in mammalian cellsDepositorInsertNUP133 (NUP133 Human)
TagsEGFPExpressionMammalianMutationdeletion of Nup133 AA1-7PromoterCMVAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
DsRed-p150 217–548
Plasmid#51146PurposeInhibits dynein-dynactin interaction in mammalian cellsDepositorAvailable SinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor
Plasmid#86843PurposeHR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.DepositorInsertFLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
TagsFLAG-HaloTagExpressionMammalianPromoterEndogenous TERT promoterAvailable SinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME-QF2
Plasmid#83307Purposecodes for QF2 (truncated QF) in a gateway middle entry backbone.DepositorInsertQF
Available SinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAMBIA-FLS2prom::FLS2-3xmyc-GFP
Plasmid#86157PurposeFLS2-GFP expresssionDepositorAvailable SinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
UBXD1-GFP
Plasmid#86464PurposeExpression of human UBXD1 with C-terminal GFP tagDepositorAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pITGA4-GFP
Plasmid#81178PurposeMammalian expression of human full length ITGA4-EGFPDepositorInsertITGA4 (ITGA4 Human)
TagsEGFPExpressionMammalianMutationContains KLGFFKR motif; A1002L (please see deposi…PromoterCMVAvailable SinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Rab21-Q76L (CA)
Plasmid#83422PurposeMouse Rab21 mutant that has defects in GTP hydrolysis (GTP-locked, constitutively active). Generated from pEGFP-Rab21 by site-directed mutagenesis.DepositorInsertRab21 (Rab21 Mouse)
TagsEGFPExpressionMammalianMutationResidue 76 from Q to L (Q76L: CAG -> CTG)PromoterCMVAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
mbYFPQS
Plasmid#80742Purposemembrane-targeted biosensor for monitoring changes in intracellular chlorideDepositorInsertYFPQS
Tagspalmitoylation signal sequence of neuromodulinExpressionMammalianMutationH148Q (CAC to CAG) and V163S (GTG to AGC).PromoterCMVAvailable SinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash1
Plasmid#74129PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 40 of Arrestin3.DepositorInsertArrestin3
TagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…Available SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-hDF
Plasmid#72584Purposehuman dysferlin full length tagged with YFPDepositorAvailable SinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only