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pCX-V5-H7N9-NS1

Plasmid

#99022

Purpose

Mammalian expression of NS1

Depositor

Honglin Chen

Publication

Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

Insert

NS1

Tags

V5

Expression

Mammalian

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pThy1-Flpbow1

Plasmid

#45180

Depositor

Joshua Sanes

Publication

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Insert

pThy1-Flpbow1

Use

Linearized for transgenic mouse injection

Promoter

Thy1

Available since

Nov. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pSBY1_FnCpf1cg

Plasmid

#104622

Purpose

CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn CPf1

Depositor

Sheng Yang

Publication

Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

Insert

FnCpf1

Use

CRISPR

Expression

Bacterial

Mutation

codon-optimized C.glutamicum

Promoter

hsp60

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223_LIG1_WT_V5

Plasmid

#83006

Purpose

Gateway Donor vector containing LIG1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

LIG1 (LIG1 Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAC90-pmax-DEST

Plasmid

#48222

Purpose

Gateway Destination vector derived from Clontech's pmax (CAGGS) expression vector

Depositor

Rudolf Jaenisch

Publication

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

CRISPR and Gateway Cloning

Expression

Mammalian

Promoter

CAGGS

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Lenti_sgRNA(MS2)_zsGreen1

Plasmid

#138463

Purpose

lenti sgRNA cloning backbone with MS2 loops and EF1a-zsGreen1

Depositor

Jian Xu

Publication

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

zsGreen1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1A

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pMK33/Cas9

Plasmid

#133781

Purpose

Expresses drosophila-optimized Cas9

Depositor

Norbert Perrimon

Publication

Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

Insert

Drosophila-optimized Cas9

Expression

Insect

Available since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pLMB509

Plasmid

#40084

Depositor

Philip Poole

Publication

Tett et al Appl Environ Microbiol. 2012 Oct;78(19):

Inserts

gfpmut 3.1
tauR
tau promoter

Expression

Bacterial

Available since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

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5` CC: Puro – loxP – GFP-C

Plasmid

#219563

Purpose

5` circularization cassette.Used in combination with one of the 3' circularization cassettes to induce Cre-mediated circularizazion or inversion of a desired genomic region.

Depositor

Andrea Ventura

Publication

Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

Insert

hPGK-promoter_PuroR_2A_loxP_GFP-C

Use

Unspecified

Promoter

hPGK

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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HBA1 full 3'UTR-T4 td downstream intron

Plasmid

#189974

Purpose

Part 6 plasmid to introduce the HBA1 full 3'UTR and T4 td downstream intron into circRNAs

Depositor

Howard Chang

Publication

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

HBA1 full 3'UTR-T4 td downstream intron

Use

Cloning

Promoter

N/A

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLF3FShC

Plasmid

#153083

Purpose

RMCE integration vector with self excising cassette

Depositor

Michael Nonet

Publication

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Type

Empty backbone

Use

Recombination mediated cassette exchange

Expression

Worm

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAVS1-shRNA

Plasmid

#82697

Purpose

For targeting shRNA construct into human AAVS1 locus, using genome editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under U6 promoter, flanked by AAVS1 homology arms.

Depositor

Alan Mullen

Publication

Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

Type

Empty backbone

Use

RNAi

Promoter

hPGK, U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCSCX

Plasmid

#63918

Purpose

Expression of the sucrose utilization phenotype

Depositor

Claudia Vickers

Publication

Bruschi et al Biotechnol Adv. 2012 Sep-Oct;30(5):1001-

Insert

cscAKB

Use

Bacterial cloning vector

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pRL128

Plasmid

#40180

Depositor

Eric Cascales

Publication

Gueguen et al Appl Environ Microbiol. 2013 Jan;79(1):3

Inserts

Ptac-lacO
araC-pBAD

Promoter

BAD and tac

Available since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pNHEJ-recX-sacB

Plasmid

#158718

Purpose

CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recX.

Depositor

Yi-Cheng Sun

Publication

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

recX

Expression

Bacterial

Promoter

Pmyc

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-mCherry-EF1 Hygro

Plasmid

#129440

Purpose

express mCherry

Depositor

Oskar Laur

Insert

mcherry

Use

Lentiviral

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pENTR-p14FAST

Plasmid

#50025

Purpose

pENTR vector for reptilian orthoreovirus p14 fusion-associated small transmembrane (FAST) protein

Depositor

Jamie Davies

Publication

Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

Insert

p14FAST

Use

Gateway Cloning

Available since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pE4n

Plasmid

#17458

Purpose

modified pENTR vector for N-terminal GST tag fusion with your gene of interest

Depositor

Giovanna Benvenuto

Publication

Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

Type

Empty backbone

Use

Modified gateway entry vector

Tags

GST and Thrombin site

Expression

Bacterial, Insect, Mammalia…

Available since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.4_StrepII_TEV_PP2Ac

Plasmid

#217477

Purpose

Expresses PP2Ac with a TEV cleavable N-terminal StrepII tag

Depositor

Wolfgang Peti

Publication

Padi et al Nature. 2024 Jan;625(7993):195-203. doi:

Insert

PP2Ac (PPP2CA Human)

Tags

StrepII, TEV

Expression

Mammalian

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE23

Plasmid

#90365

Purpose

ATF4 - Amino acid deprivation gene reporter

Depositor

Ramnik Xavier

Publication

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

AARE

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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