We narrowed to 66,118 results for: nin

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35S-VP16-GWY/pAM

Plasmid

#201234

Purpose

Used to produce and express in planta C-terminal fusion proteins with the VP16 activation domain

Depositor

Michael Ayliffe

Publication

Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

Insert

35S-VP16-GWY

Expression

Plant

Promoter

CaMV 35S

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CROPseq-EFS-HypaSpCas9-P2A-puro

Plasmid

#106965

Purpose

single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

Depositor

Alex Hewitt

Insert

HypaSpCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EFS

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCW-Flag-CtIP-T847E

Plasmid

#71110

Purpose

mammalian expession of Flag-CtlP T847E mutation

Depositor

Daniel Durocher

Publication

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

CtlP

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

T847E

Promoter

TRE promoter, Tet ON

Available since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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shCTNNB1 # 2

Plasmid

#42544

Depositor

William Hahn

Publication

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

CTNNB1 (CTNNB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLDPuro-hsnSR100N

Plasmid

#35172

Depositor

Benjamin Blencowe

Publication

Raj et al Mol Cell. 2011 Sep 2;43(5):843-50.

Insert

SRRM4 (SRRM4 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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HNF1B_pLX307

Plasmid

#98342

Purpose

Lentiviral expression of HNF1B

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

HNF1B (HNF1B Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pBLADE(FP6**)-mCherry

Plasmid

#168049

Purpose

Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter. mCherry expression is controlled by the PBAD promoter.

Depositor

Barbara Di Ventura

Publication

Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

Inserts

Blue light-inducible AraC dimers in Escherichia coli
mCherry

Expression

Bacterial

Promoter

J23101** and PBAD

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP3

Plasmid

#158354

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP3 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA-FLAG-Hu-Trip6

Plasmid

#27255

Depositor

Thomas Gilmore

Publication

Zhao et al Gene Expr. 1999 . 8(4):207-17.

Insert

Trip6 (TRIP6 Human)

Tags

FLAG

Expression

Mammalian

Available since

Feb. 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
SV40 basal promoter-GFP-IRES-AP

Plasmid

#18807

Depositor

Connie Cepko

Publication

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

SV40 basal promoter-GFP-IRES-AP

Expression

Mammalian

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
SPACE-NG (pRZ5150)

Plasmid

#140244

Purpose

CMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

Depositor

Keith Joung

Publication

Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

SPACE_NG

Expression

Mammalian

Mutation

V82G in TadA*, NG mutations in SpCas9, D10A in Sp…

Promoter

CMV

Available since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLVX-EF1a-EGFP-ERGIC53-IRES-Puromycin

Plasmid

#134859

Purpose

Expresses EGFP-tagged ERGIC marker

Depositor

David Andrews

Publication

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

ERGIC53 (Lman1 Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAG306GPD-ccdB

Plasmid

#14140

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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SECURE BE3(R33A)-P2A-EGFP (pJUL1085)

Plasmid

#123614

Purpose

CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).

Depositor

Keith Joung

Publication

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3(R33A)-P2A-EGFP

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in SpCas9

Promoter

CAG

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-3-SARS-CoV-2-3CL

Plasmid

#168457

Purpose

Bacterial codon optimized expression vector for SARS-CoV-2 3CL protease.

Depositor

Alejandro Chavez, David Ho, Sho Iketani

Publication

Iketani et al Nat Commun. 2021 Apr 1;12(1):2016. doi:

Insert

SARS-CoV-2 3CL protease (ORF1ab Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

March 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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pAtetO_6xHis-Klentaq

Plasmid

#157862

Purpose

Plasmid for the aTc-inducible expression of the Klentaq

Depositor

Andrew Ellington

Publication

Milligan et al Biochemistry. 2018 Aug 7;57(31):4607-461

Insert

Klentaq

Tags

6xHis

Expression

Bacterial

Promoter

tet PA

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-eA3A-BE5-NG-BlastR

Plasmid

#152998

Purpose

High purity C-to-T base editor

Depositor

David Liu

Publication

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

eA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1-GGamma1-GFP2

Plasmid

#140989

Purpose

Encodes a G gamma subunit (GNGT1) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Publication

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GGamma1-GFP2 (GNGT1 Human)

Tags

GFP2 and GSAG linker

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Mitochondrial cpGFP

Plasmid

#215711

Purpose

Expresses cpGFP in the mitochondria

Depositor

Katharine Diehl

Publication

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

cpGFP

Use

Lentiviral

Tags

FLAG, HA, and mitochondrial localization sequence…

Expression

Mammalian

Mutation

circularly permuted GFP

Promoter

CMV

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-Arl13b-mCardinal

Plasmid

#194542

Purpose

Expresses Arl13b with mCardinal in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Publication

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Insert

Arl13b (Arl13b Mouse)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV IE94

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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