164,220 results

1. pCS2FA-H2B-mScarlet3

   Plasmid

   #224436

   Purpose

   Construct for labelling cell nuclei in mammalian cells using Histon protein 2B fused to mScarlet3

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   H2B-mScarlet3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV & SP6

   Available Since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. p-dCas9-SSAP-MS2-BB_BbsI

   Plasmid

   #183826

   Purpose

   pU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFP

   Depositor

   Le Cong

   Article

   Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

   Insert

   U6-guideRNA-dCas9-T2A-EBFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBH

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSpliceExpress

   Plasmid

   #32485

   Depositor

   Stefan Stamm

   Article

   Kishore et al Gene. 2008 Dec 31;427(1-2):104-10. Epub

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCIpA102-G-HLA-A2_GFP

   Plasmid

   #85162

   Purpose

   for mRNA expression of HLA.A2-EGFP

   Depositor

   Sébastien Walchli, Tone Fredsvik Gregers

   Article

   Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

   Insert

   HLA.A2 (HLA-A Human)
   

   Use

   Mrna expression

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMZ-180

   Plasmid

   #195428

   Purpose

   Transient, constitutive expression of synZiFTR: ZF5-p65

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   pUb - 3XFLAG - NLS - ZF5-7 (ZF5) - p65 - RbGpA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-EF1α-F-FLEX- Kir2.1-T2A-tdTomato

   Plasmid

   #60661

   Purpose

   Flp-dependent expression of Kir2.1-T2A-tdTomato

   Depositor

   Massimo Scanziani

   Article

   Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

   Insert

   FLEX- Kir2.1-T2A-tdTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1α

   Available Since

   Nov. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. CaMKIIP-mEGFP-P2A-paAIP2/pAAV

   Plasmid

   #91718

   Purpose

   AAV-mediated expression of mEGFP and paAIP2 for optogenetic control of CaMKII

   Depositor

   Ryohei Yasuda

   Article

   Murakoshi et al Neuron. 2017 Apr 5;94(1):37-47.e5. doi:

   Insert

   mEGFP-P2A-paAIP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CaMKII 1.3

   Available Since

   June 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDB4581

   Plasmid

   #171124

   Purpose

   A kanMX-marked pFA6a based plasmid, which has a 7-aa linker, a 16-aa XTEN16 linker, three tandam repeats of sAID. It serves as the template for 3_sAID degron tagging at the C-terminus.

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2022 Jan 4;12(1):jkab393.

   Insert

   3_sAID
   

   Use

   Other

   Tags

   Expression

   Mutation

   non applicable

   Promoter

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 Flag C/EBPa

   Plasmid

   #66978

   Purpose

   Expression plasmid for mouse C/EBPa; adds Flag tag to the N-terminus of protein

   Depositor

   Christopher Vakoc

   Article

   Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

   Insert

   C/EBP alpha (Cebpa Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EF.STAT3DN.Ubc.GFP

    Plasmid

    #24984

    Purpose

    Depositor

    Linzhao Cheng

    Article

    Hillion et al Cancer Research 68, 10121, December 2008

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y705F--dominant negative

    Promoter

    Available Since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-GRAB-rDA3m

    Plasmid

    #208702

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neurons

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Has Service

    AAV1

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA3m
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-PCNA

    Plasmid

    #215117

    Purpose

    Expresses mCherry-PCNA in mammalian cells from a lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    PCNA (PCNA Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-hRARβ.hRXRα

    Plasmid

    #135415

    Purpose

    Human Retinoic Acid Receptor-beta and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector

    Depositor

    Catharine Ross

    Article

    Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

    Inserts

    Human retinoic acid receptor-beta
    Human retinoid X receptor-alpha
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and SV40

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBS-hsFlt3L

    Plasmid

    #25074

    Depositor

    Maria Castro

    Article

    Curtin et al J Immunol. 2006 Mar 15. 176(6):3566-77.

    Insert

    human soluble FMS-like tyrosine kinase 3 ligand (FLT3LG Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKB01_mCherry

    Plasmid

    #69104

    Purpose

    Expresses mCherry (codon adapted for Bacillus subtilis); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

    Depositor

    Jan-Willem Veening

    Article

    Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRN112

    Plasmid

    #84464

    Purpose

    pJB38-NWMN29-30+ SarA_P1-mAmetrine-Term

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    SarA_P1-mAmetrine from pRN12
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMycVec2

    Plasmid

    #32361

    Depositor

    Michael Niederweis

    Article

    Kaps et al Gene. 2001 Oct 31;278(1-2):115-24.

    Insert

    pJAZ38 ori
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAC-VIOL

    Plasmid

    #53087

    Purpose

    Produces the carotenoid violaxanthin in E. coli.

    Depositor

    Francis X Cunningham Jr

    Article

    Neuman et al Plant J. 2014 Apr;78(1):80-93. doi: 10.1

    Insert

    zep (ABA1 Mustard Weed)
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Lacks codons for the first 60 N-terminal amino ac…

    Promoter

    T7

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSL1-GFP

    Plasmid

    #180420

    Purpose

    Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a GFP promoter.

    Depositor

    Nancy Moran

    Article

    Li et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AP1muA-Halo-ALFA-polyA-G418 HR

    Plasmid

    #229681

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    Halo, ALFA

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TFORF2158

    Plasmid

    #141949

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    BCL11B (BCL11B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET-28a (+)-hTBP

    Plasmid

    #171073

    Purpose

    Expresses human TBP in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TBP

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Insert

    TATA-box-binding protein (TBP Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pQCXIZ GFP PODXL A WT

    Plasmid

    #202413

    Purpose

    Expression of GFP-tagged PODXL (isoform A) WT

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    PODXL (PODXL Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. UbcH5c S22R pET28a

    Plasmid

    #12644

    Depositor

    Rachel Klevit

    Article

    Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

    Insert

    Ubch5c - Ubiquitin conjugating enzyme (UBE2D3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 31, 2007

    Availability

    Academic Institutions and Nonprofits only

25. pcDNA3-Dub3 mouse

    Plasmid

    #72682

    Purpose

    mammalian expression of mouse Dub3

    Depositor

    Domenico Maiorano

    Article

    van_der_Laan et al Mol Cell. 2013 Nov 7;52(3):366-79. doi:

    Insert

    Dub3 (Usp17le Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

26. miniCMV-(mNeonGreen)4-tDeg

    Plasmid

    #129402

    Purpose

    (mNeonGreen)4-tDeg fluorogenic protein

    Depositor

    Samie Jaffrey

    Article

    Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

    Insert

    (mNeonGreen)-tDeg
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p101_CRISPRai_PiggyBac

    Plasmid

    #213777

    Purpose

    Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.

    Depositor

    Howard Chang

    Article

    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Inserts

    VPR-dSaCas9
    dSpCas9-KRAB-BFP
    zeocin
    Tet-On transactivator
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EF1a (EF-1-alpha intron a) and Tet_On_TRE

    Available Since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAXY0002

    Plasmid

    #179835

    Purpose

    binary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoter

    Depositor

    Xiaohan Yang

    Article

    Yuan et al Hortic Res. 2021 Nov 1;8(1):234. doi: 10

    Type

    Empty backbone

    Use

    Binary vector for plant transformation

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMN-013

    Plasmid

    #195417

    Purpose

    AAVS1 Integratable mCherry Reporter for synZiFTR ZF5

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    4X ZF5 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

    Plasmid

    #225756

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMC-6-ZmBbm-9

    Plasmid

    #197748

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    ZmBbm
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pD864-LacZ

    Plasmid

    #109380

    Purpose

    Rhamnose inducible LacZ expression. Used to test burden response in E. coli

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pRHAM-LacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. CLYBL_Bxb1-GA_LP_Dual_TC

    Plasmid

    #229776

    Purpose

    CLYBL targeting construct with the Bxb1-GA landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    FRT-mScarlet-attP(GA)-PuroR-F3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pGK

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. HCT160_pAAV-3X-eHGT_1139m-minBGprom-iCre297T-WPRE3-bGHpA

    Plasmid

    #233559

    Purpose

    Direct-expressing iCre297T AAV virus

    Depositor

    Allen Institute for Brain Science, Tanya Daigle

    Article

    Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

    Insert

    iCre297T
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBglobin

    Available Since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro

    Plasmid

    #138152

    Purpose

    Expresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    eGFP-MCS
    

    Use

    Lentiviral

    Tags

    2PEST AND NLS

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pT7-TaGu-ENT-3xF-ORF

    Plasmid

    #238488

    Purpose

    For production of C-term flag-tagged TaGu EN-tuned ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Expression

    Mutation

    R1109->A

    Promoter

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-CAG-miniGFP2-P2A-FusionRed

    Plasmid

    #188734

    Purpose

    Express miniGFP2 and FusionRed in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    miniGFP2
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTrc-trGPPS(CO)-LS

    Plasmid

    #50603

    Purpose

    pTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthase

    Depositor

    Jay Keasling

    Article

    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

    Inserts

    Limonene Synthase
    ApR
    LacIq
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    truncated codon optimized geranylpyrophosphate sy…

    Promoter

    Trc

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMU-ERr

    Plasmid

    #61170

    Purpose

    Fluorescent reporter for endoplasmic reticulum AtWAK2sp-mCherry-HDEL expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    AtWAK2-mCherry-HDEL
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available Since

    April 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCAG_Xph20-eGFP-CCR5TC

    Plasmid

    #135530

    Purpose

    Encodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph20
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge