We narrowed to 14,052 results for: CRISPR-Cas9

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pMLS285

Plasmid

#73734

Purpose

SapTrap egl-13 NLS connector donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

egl-13 NLS

Use

CRISPR

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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psLIB TRE mito-mCherry

Plasmid

#229966

Purpose

encodes a mCherry targeted to mitochondria under the control of a dox inducible promoter

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

mito-mCherry

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-GFP sgRNA Target 3- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

Plasmid

#210739

Purpose

Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 3

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Inserts

Sp Cas9 (cas9 S pyogenes)
Sp sgRNA GFP Target 3

Use

CRISPR

Tags

3xFLAG, SV40 NLS, PolyA signal

Expression

Mammalian

Promoter

H1 and eCMV

Available Since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-GFP sgRNA Target 1- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

Plasmid

#210737

Purpose

Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 1

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Inserts

Sp Cas9 (cas9 S pyogenes)
Sp sgRNA GFP Target 1

Use

CRISPR

Tags

3xFLAG, SV40 NLS, PolyA signal

Expression

Mammalian

Promoter

H1 and eCMV

Available Since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-GFP sgRNA Target 2- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

Plasmid

#210738

Purpose

Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 2

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Inserts

Sp Cas9 (cas9 S pyogenes)
Sp sgRNA GFP Target 2

Use

CRISPR

Tags

3xFLAG, SV40 NLS, PolyA signal

Expression

Mammalian

Promoter

H1 and eCMV

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-GFP sgRNA Target 4- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

Plasmid

#210740

Purpose

Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 4

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Inserts

Sp Cas9 (cas9 S pyogenes)
Sp sgRNA GFP Target 4

Use

CRISPR

Tags

3xFLAG, SV40 NLS, PolyA signal

Expression

Mammalian

Promoter

H1 and eCMV

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-hCASeGFP-pA

Plasmid

#59766

Purpose

Expresses CAS9-eGFP fusion protein in mammalian cells.

Depositor

Wataru Fujii

Article

Fujii et al Nucleic Acids Res. 2013 Aug 30.

Insert

CAS9

Tags

Flag and eGFP

Expression

Mammalian

Promoter

CAG

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pG1

Plasmid

#162602

Purpose

Edit Ade2 gene in yeast

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Expression

Yeast

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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M-SP-sgRNA

Plasmid

#48671

Purpose

Mammalian U6-driven sgRNA (SPm) targeting GTCCCCTCCACCCCACAGTG

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. pyogenes Cas9, hU6 promoter

Use

CRISPR

Promoter

hU6

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pG2

Plasmid

#162603

Purpose

Edit Ade2 gene in yeast

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Expression

Yeast

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pG3

Plasmid

#162604

Purpose

Edit Ade2 gene in yeast

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Expression

Yeast

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pG4

Plasmid

#162605

Purpose

Edit Ade2 gene in yeast

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Expression

Yeast

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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M-NM-sgRNA

Plasmid

#48673

Purpose

Mammalian U6-driven sgRNA (NMm1) targeting GTCCCCTCCACCCCACAGTG

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with N. meningitidis Cas9, hU6 promoter

Use

CRISPR

Promoter

hU6

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pBLO1808_arC9_2xNLS_human

Plasmid

#74491

Purpose

Human codon optimized plasmid to express arC9 t2a mCherry w/2xNLS and a sgRNA

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

arC9

Tags

t2a mCherry

Expression

Mammalian

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pLX-sgRNA

Plasmid

#50662

Purpose

Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

Depositor

Eric Lander, David Sabatini

Article

Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

Insert

sgAAVS1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pLV GG hUbC-dsRED

Plasmid

#84034

Purpose

Contains Golden Gate cassette to express up to four gRNAs and dsRED

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pAF-HdrB3

Plasmid

#184908

Purpose

carrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferridurans

Depositor

Radhakrishnan Mahadevan

Article

Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

Insert

Cas9-sgRNA-homology arms

Use

CRISPR; Broad-host-range

Expression

Bacterial

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pTargetF

Plasmid

#62226

Purpose

Constitutive expression of sgRNA without donor editing template DNA

Depositor

Sheng Yang

Article

Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

Insert

sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pij23119

Available Since

March 24, 2015

Availability

Academic Institutions and Nonprofits only

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phH1-gRNA

Plasmid

#53186

Purpose

Expresses the S. pyogenes sgRNA from the H1 promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

human H1

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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ph7SK-gRNA

Plasmid

#53189

Purpose

Expresses the S. pyogenes sgRNA from the 7SK promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

human 7SK

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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