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We narrowed to 18,759 results for: nls

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Showing: 9721 - 9760 of 18759 results

  2. ttHsCas12a

    Plasmid
    #182385
    Purpose
    Gives high editing efficiency in barley.
    Depositor
    Wendy Harwood
    Publication
    Lawrenson et al bioRxiv 2022.04.28.489853
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ttHsCas12a
    Use
    CRISPR; Coding sequence only
    Mutation
    D156R
    Promoter
    None
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182385-plasmid-map-sequence-id-361473

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  6. cPE

    Plasmid
    #183549
    Purpose
    Expresses compact Prime Editor in mammalian cells
    Depositor
    Wen Xue
    Publication
    Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 nickase (H840A), Reverse Transcriptase
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    183549-plasmid-map-sequence-id-365389

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  10. pFGC-I2Cas9

    Plasmid
    #173158
    Purpose
    Expresses Cas9 in dividing Arabidopsis cells. Contains dsRED and Basta for fast transformant selection.
    Depositor
    Piotr Ziolkowski
    Publication
    Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:
    Inserts
    hSpCas9
    pNAP:dsRED:tNOS
    pMAS:BAR:tMAS
    ICU2 upstream regulatory region
    Tags
    3xFLAG-NLS and NLS
    Expression
    Plant
    Mutation
    Contains a D169N mutation with no visible effect …
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    173158-plasmid-map-sequence-id-358594

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  11. pSLQ10714

    Plasmid
    #183959
    Purpose
    U6-crKlf4-CAG-hyperdCas12a-miniVPR
    Depositor
    Stanley Qi
    Publication
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    HyperdCas12a
    crRNA targeting Klf4
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CAG and U6
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183959-plasmid-map-sequence-id-363110

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  31. pMP926

    Plasmid
    #135001
    Purpose
    m6A Reader-Writer. Propagates Gm6ATC along GATC arrays as well as across cell generations. pUBC-DpnI(aa146-254)-3xFLAG-Dam(R95A) pGK-HygroR
    Depositor
    Ahmad Khalil
    Publication
    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.
    Insert
    pUBC-DpnI(aa146-254)-3XFLAG-Dam(R95A) pGK-HygroR
    Use
    Lentiviral and Synthetic Biology
    Tags
    3xFLAG, Dam(R95A), and DpnI(aa146-254)
    Expression
    Mammalian
    Mutation
    DpnI domain truncated to aa146-254. Dam protein m…
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    135001-plasmid-map-sequence-id-267929

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  37. bU6-sgMark2-mU6-sgMark3-hU6-sgMark4

    Plasmid
    #177235
    Purpose
    Expresses Mark2 (bU6), Mark3 (mU6) and Mark4 (hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgMark2/sgMark3/sgMark4
    Use
    Lentiviral
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    177235-plasmid-map-sequence-id-352696

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  40. AAVS1_Puro_hPGK1_mScarlet-I-TOSI_Donor

    Plasmid
    #178087
    Purpose
    HDR donor plasmid to introduce the mScarlet-I mTOR signaling indicator (TOSI) cassette to AAVS1 using AAVS1 T2 sgRNA.
    Depositor
    Yannick Doyon
    Publication
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mScarlet-I mTOR signaling indicator (TOSI)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Human PGK1
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178087-plasmid-map-sequence-id-350132

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Showing: 9721 - 9760 of 18759 results