We narrowed to 18,681 results for: tec

1. pABM-D4-miR30-L1221

   Plasmid

   #132933

   Purpose

   Lentivector for luciferase (control) knockdown and blasticidin resistance

   Depositor

   Jeremy Luban

   Article

   Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

   Insert

   luciferase shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-ML1N*2(7Q)

   Plasmid

   #67679

   Purpose

   Multisite gateway entry clone for adding C-terminal fusions of p3E-ML1N*2(7Q)

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   ML1N*2(7Q)
   

   Use

   Gateway multisite 3' entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. fADL-1e-flag-linker-p3

   Plasmid

   #139441

   Purpose

   For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage.

   Depositor

   Alexander Gabibov

   Article

   Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

   Insert

   linker with pelB-leader, cloning site, flag-tag and p3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFPL-Yh2

   Plasmid

   #61650

   Purpose

   Gateway vector for generating yellow fluorescent protein fusions in fungi

   Depositor

   Mark Farman

   Article

   Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

   Insert

   enhanced yellow fluorescent protein
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pAM/CBA-eGFP-miRaSyn(mouse)x3-WPRE-bGHpA

   Plasmid

   #194248

   Purpose

   Expresses 3 miRNAs targeting mouse alpha-Synuclein

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   miRaSynuclein(mouse) (Snca Mouse)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTH-PanCen TALE-mVenusN

   Plasmid

   #191395

   Purpose

   TALE fused to N-terminal part of split mVenus

   Depositor

   Albert Jeltsch

   Article

   Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

   Insert

   N-terminal part of split mVenus
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOTF3-AK (clone3)

   Plasmid

   #21165

   Depositor

   James Thomson

   Article

   Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

   Insert

   POUF5F1 (genomic DNA) (POU5F1 Human)
   

   Use

   Cre/Lox; Homologous recombination

   Tags

   Expression

   Mutation

   NeoR, EGFP, and SV40 polyA are between the two ge…

   Promoter

   Available since

   June 25, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pASKt15C+SrtA7woH

   Plasmid

   #65020

   Purpose

   Expresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

   Insert

   sortase A
   

   Use

   Tags

   S tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, P94R, E105K, E108A, D16…

   Promoter

   tetracycline promoter/operator

   Available since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCfB6684

   Plasmid

   #106154

   Purpose

   EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6631, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCfB6631

    Plasmid

    #106161

    Purpose

    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6684 into Yarrowia lipolytica chromosomal location IntD_1, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    unknown
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUCBB-pBAD-eGFP

    Plasmid

    #32553

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 FLIPE-10u

    Plasmid

    #13542

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E A207R
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    A207R in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKH-MoHyPer

    Plasmid

    #78267

    Purpose

    Express HyPer-AS sensor that change excitation wavelength with the presence of hydrogen peroxide.

    Depositor

    Nicole Donofrio, Jeffrey Caplan

    Article

    Huang et al Mol Plant Pathol. 2016 Mar 7. doi: 10.11

    Insert

    MoHyper
    

    Use

    Express in magnaporthe

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Rp27

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pDisplay FLIPE-1m

    Plasmid

    #13548

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E A207W
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    A207W in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEF-HA/Net1A

    Plasmid

    #28262

    Depositor

    Jeffrey Frost

    Article

    Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

    Insert

    Net1A (Net1 Mouse)
    

    Use

    Tags

    2x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pCDNA3.1(+)-HaloTag N58-(GGS)X6-hH2A

    Plasmid

    #171785

    Purpose

    Expression of HaloTag N58-(GGS)X6-hH2A in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    HaloTag N58-(GGS)X6-hH2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSP2266

    Plasmid

    #70705

    Purpose

    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCellFree_G03 PDGFRB

    Plasmid

    #67130

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PDGFRB (PDGFRB Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pDisplay FLIPE-10u

    Plasmid

    #13546

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E A207R
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    A207R in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. Dppa1-T2A-H2B-Halo donor

    Plasmid

    #113118

    Purpose

    Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Dppa1 donor region including homology arms and T2A-H2B-Halo
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge