9,154 results
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Plasmid#121178PurposeN-terminal taggingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHygro-P2A-mAID
UseCRISPRAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Tandem p40p35
Plasmid#108665PurposeExpression of bioactive mouse IL-12 as a tandem dimer of p40 and p35 linked with a Gly-Ser linkerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianMutationIL-12 dimers have a Gly-Ser linkerAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pXPR_047
Plasmid#107145Purposemeasures Cas9 activityDepositorInsertGFP + sgRNA for GFP
UseCRISPR and LentiviralExpressionMammalianAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCI-neo Fluc EGFP
Plasmid#90170PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteinsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFLuc EGFP
ExpressionMammalianAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCI-neo FlucDM EGFP
Plasmid#90172PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFLuc EGFP
ExpressionMammalianMutationR188Q, R261Q in FLucAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP_sfCherry2(11)_H2B
Plasmid#82605PurposeExpresses sfCherry2(11) tagged H2B in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsfCherry2(11)_H2B
ExpressionMammalianMutationchanged Gly12 to Ala in sfCherry11+Available sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA
Plasmid#86613PurposeVector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmidDepositorInsertATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPR; Co-selection via hdr using ouabainExpressionMammalianMutationK848A, K1003A, & R1060APromoterCBhAvailable sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pON.mCherry
Plasmid#84821PurposeConstitutive Expression plasmid with mCherry fluorescent proteinDepositorInsertmCherry
ExpressionBacterialPromoterTac PromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti Lifeact-iRFP670 BlastR
Plasmid#84385PurposeLentiviral expression of iRFP670-tagged Lifeact with Blasticidin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsiRFP670PromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-TA-ERN
Plasmid#80474PurposeAll-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and neomycin resistance)DepositorTypeEmpty backboneUsePiggybac transposonExpressionMammalianAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ASAP2f
Plasmid#83960PurposeFluorescent reporter for voltage imagingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertASAP2f
ExpressionMammalianPromoterCAGAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hDlx-GiDREADD-dTomato-Fishell-5
Plasmid#83896PurposeGi-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer elementDepositorHas serviceAAV1, AAV9, and AAV RetrogradeInserthM4Di
UseAAVExpressionMammalianPromoterhDlxAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLentiCas9-GFP
Plasmid#78546PurposeFusion protein Cas9+GFPDepositorInsertGFP
UseLentiviralExpressionMammalianPromoterFusion protein with Cas9Available sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
N174-MCS (Puro)
Plasmid#81068PurposeLentiviral vector for constitutive gene expression.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterEF-1αAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBAD-smURFP-RBS-HO-1
Plasmid#80341PurposeExpresses smURFP and Heme Oxygenase-1 in bacterial cells.DepositorInsertssmURFP
HO-1
Tags6 Histidine TagExpressionBacterialPromoterpBADAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGAMA-YAP
Plasmid#74942PurposeLentiviral over-expression vector for YAPDepositorAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcdna nesprin HL
Plasmid#68128Purposenesprin headless controlDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnesprin-2 (Syne2 Mouse)
ExpressionMammalianMutationdeleted actin-binding domainPromoterCMVAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1_AktPH-mCherry
Plasmid#67301PurposeFluorescent indicator for PI(3,4,5)P3DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAktPH-mCherry (AKT1 Human, Synthetic)
ExpressionMammalianPromoterCMVAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p0521s
Plasmid#62862PurposeEpisome vector for Phaeodactylum tricornutum (pCC1BAC-based)DepositorTypeEmpty backboneUseSynthetic Biology; Episomal vector for phaeodacty…Available sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LBR pEGFP-N2 (646)
Plasmid#61996Purposemammalian expression of nuclear envelope transmembrane proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLBR (LBR Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by