We narrowed to 18,759 results for: nls
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Plasmid#173025PurposeEncodes OptoAMP1 circuitDepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
EZ-L439
Plasmid#169146PurposeEncodes OptoINVRT7 circuitDepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
TMED3-hLOV-TEVcs-GAL4bd-VP64-V5
Plasmid#170996PurposeHiLITR transcription factor with full-length TMED3 targeting sequence (ER/Golgi)DepositorInsertTMED3(FL)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (TMED3 Synthetic)
UseLentiviral and Synthetic BiologyTagsV5ExpressionMammalianPromoterEf1-alphaAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIVTR(T7A)-Cas9-T2A-mClover3
Plasmid#172293PurposeIn vitro transcription of Cas9-T2A-mClover3 synthetic constructDepositorInsertCas9-T2A-mClover3
UseCRISPRPromoterT7 promoter A-initiatingAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330 SUZ12 - pDY083
Plasmid#171109PurposeExpresses Cas9 and an sgRNA targeting SUZ12DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9
ExpressionMammalianAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU51
Plasmid#167677PurposeFinal destination vector for single Gateway LR reactionDepositorInsertPcUB4-2::spCas9
UseCRISPRAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU52
Plasmid#167678PurposeFinal destination vector for single Gateway LR reactionDepositorInsertPcUB4-2::spCas9
UseCRISPRAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti HsATP13A2 D962N
Plasmid#171821Purposetransfer plasmid for lentiviral vector production expressing Hs ATP13A2 D962NDepositorAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV.U6.gDNMT1.Cas9-T2A-GFP
Plasmid#170362PurposePlasmid used for Crispr/cas9 based disruption of human DNMT1.DepositorInsertCas9-T2A-GFP
UseLentiviralAvailable sinceJune 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYZ673
Plasmid#132953PurposecrRNA entry vector of Pfba1-BsaI pad-TEF1 Term with FnCas12a in Schizosaccharomyces pombeDepositorInsertFnCas12a
UseCRISPR and Synthetic BiologyPromoteradh1Available sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
miniTol2 x EF1a_EKAREN5(TA) + PGK-puro
Plasmid#167819Purposecontrol sensor for EKAREN5DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEKAREN5(TA)
Tagsnls localization motifExpressionMammalianMutationK424P, K426W, T420APromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV x EF1a_EKAREN4-ires-blast
Plasmid#167829PurposeOptimized EKAREV FRET biosensor sensor for ERKDepositorInsertEKAREN4
UseLentiviralTagsnls localization motifExpressionMammalianMutationK424P, K426WPromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
UAS-Ceru-10unit
Plasmid#158389Purpose10-unit intMEMOIR recording array for Drosophila melanogasterDepositorInsert10unit recording array
ExpressionInsectAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
PL-CRISPR.EFS.tRFP-gIfngr1-1
Plasmid#166479PurposeExpresses spCas9, tRFP and a gRNA targeting mouse Ifngr1DepositorInsertgRNA for mouse Ifngr1 (Ifngr1 Mouse)
UseLentiviralAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
TC872
Plasmid#164895PurposeTRE::nCre, inducible nCre expression when co-express with rtTADepositorInsertnCre
ExpressionWormAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
PL-CRISPR.EFS.tRFP-gIfngr1-2
Plasmid#166480PurposeExpresses spCas9, tRFP and a gRNA targeting mouse Ifngr1DepositorInsertgRNA for mouse Ifngr1 (Ifngr1 Mouse)
UseLentiviralAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
CL7-LbCas12aD156R
Plasmid#164659PurposeBacterial expression of mutated LbCas12aD156R using CL7/Im7DepositorInsertLbCas12a-D156R
TagsThioredoxin, CL7, SUMOExpressionBacterialMutationchanged Aspartic Acid 156 to ArgininePromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-TRE-DIO-Cas9
Plasmid#166601PurposeEncodes Cre-dependent Cas9 under control of the TREDepositorInsertCas9
UseAAVPromotertetracycline response elementAvailable sinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-STU
Plasmid#138110PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized LbCas12a without promoter and terminatorDepositorInsertLbCas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-TREX1 g1 Cas9-T2A-mCherry
Plasmid#164250PurposeTranscription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgTREX1
UseCRISPRExpressionMammalianPromoterU6Available sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGK415-luxTA2-4F
Plasmid#165971PurposeExpresses luxTA(2-4F) mutantDepositorInsertluxR
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationS116Y, W201R, codon-optimized for expression in y…PromoterpPGK1 mutantAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha1 P35S:PhiC31 integrase-RDF:T35S (GB2893)
Plasmid#160633PurposeTU for the constitutive expression of the PhiC31 integrase, C-terminally fused with its reversion factor (RDF)DepositorInsertPhiC31-RDF
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha2 P35S:dCas9:VPR:Tnos (GB1826)
Plasmid#160623PurposeTU for the constitutive expression of dCas9 fused to VPR (VP64-p65-Rta) activation domainsDepositorInsertP35S:dCas9:VPR-Tnos
ExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcna1a-IRES-EGFP
Plasmid#164965PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFPDepositorInsertkcna1a (kcna1a Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPPromoteractinb (actb2) promoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330 RBP1 gRNA
Plasmid#165593PurposeInsertion of RBP1 degronDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRBP1 (Polr2a Mouse)
ExpressionMammalianMutationNAAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330 RAD21 gRNA
Plasmid#165592PurposeInsertion of RAD21 degronDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRAD21 (Rad21 Mouse)
ExpressionMammalianMutationNAAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX459-CTNNB1-S45
Plasmid#164587PurposeExpresses a gRNA that overlaps the S45 codon of human CTNNB1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCTNNB1 gRNA (CTNNB1 Human)
UseCRISPRExpressionMammalianPromoterU6Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
AiP1046-pAAV-mscRE13-minBGpromoter-iCre-WPRE-hGHpA
Plasmid#163478PurposeDirect-expressing iCre AAV Virus. Alias: AiP1046 - pAAV-AiE2013m-minBG-iCre-WPRE-HGHpADepositorInsertiCre
UseAAVPromoterBeta Globin minimal promoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262m
Plasmid#161522PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE7.10 mediated A-G base editingDepositorInsertTadA(wt)-TadA(7.10)-zSpCas9(D10A)
UseCRISPR; Gateway compatible tada(wt)-tada(7.10)-zs…ExpressionPlantAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-ABE-3'UTR-sgRNA-2xMS2
Plasmid#132554PurposeVector plasmid expressing ABEmax and sgRNA scaffold with MS2 replacing the Tetraloop and loop 2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsABEmax
sgRNA, with Tetraloop and loop 2 replaced by MS2 aptamer
ExpressionMammalianAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::Vkg::mCherry
Plasmid#163929PurposeExpression of GrabFP-ECM under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 nanobody with Viking (Collagen IV) protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationG1767DAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cip-pAct1-Mnase
Plasmid#162556PurposeNheI-MluI-digested 3xFLAG-MNase-SV40 fragment was cloned into the CIp-pACT1-CYC vector to ensure constitutive expression of MNase in C. albicans.DepositorInsert3FLAG-Mnase-SV40
ExpressionYeastPromoterActineAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_mCherry-CD8-vhhGFP
Plasmid#163930PurposeExpression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUASTLOTattB_mCherry::Nrv1::vhhGFP4
Plasmid#163926PurposeExpression of GrabFP-B-intracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (intracellular) nanobody with Nrv1 protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2-sgAMPK alpha2 clone1
Plasmid#162122PurposeLentiviral sgRNA plasmid targeting human AMPK alpha2DepositorInsertsgAMPK alpha2 (PRKAA2 Human)
UseLentiviralAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2-sgAMPK alpha1 clone1
Plasmid#162119PurposeLentiviral sgRNA plasmid targeting human AMPK alpha1DepositorInsertsgAMPK alpha1 (PRKAA1 Human)
UseLentiviralAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgHK2_2
Plasmid#163461Purposelentiviral vector expressing Cas9 and an sgRNA targeting HK2DepositorAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330 NC-FKBP-Cas9
Plasmid#162725PurposeMammalian expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFKBP F36V
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330 NL-FKBP-Cas9
Plasmid#162723PurposeMammalian expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFKBP F36V
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD057 VP64-ZF37
Plasmid#138834PurposeConstitutive expression of VP64-ZF37 under the CMV promoter.DepositorInsertVP64-ZF37
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only