We narrowed to 17,814 results for: multi
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Plasmid#73478PurposeEncodes the G protein to enable transsynaptic RAV infectionDepositorInsertH2B-3XHA-P2A-N2c(G)
UseAAVExpressionMammalianAvailable SinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRE-BI-hGRAD
Plasmid#207837PurposeInducible hGRAD Plasmids for rapid depletion of GFP-tagged proteins in mammalian cellsDepositorInsertsExpressionMammalianMutation215 amino acids of the full length proteinPromoterTRE3G BI promoterAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-C6F/TdTomato
Plasmid#140826PurposeOCT4, SOX2, KLF4, C-MYC, KLF2, NANOG (C6F) and tdTomato under the control of TRE (Tet-ON)DepositorInsertsExpressionMammalianMutationL104P- please see depositor commentsAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2]
Plasmid#74102Purposeplasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene)DepositorInsertsXenopus laevis beta-tubulin gene promoter region and 5'UTR
Green Lantern Green Fluorescent Protein
Rabbit beta 1-globin gene 3'UTR
ExpressionBacterialAvailable SinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-hNINJ1-GFP
Plasmid#208779PurposeInducibly expresses human NINJ1 tagged with GFPDepositorAvailable SinceAug. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_GEM2-Halo-FRB
Plasmid#197056PurposeExpression of Synechococcus elongatus encapsulin Halo-FRB-fusion in mammalian cellsDepositorInsertEncapsulin
TagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionAvailable SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TK-Firefly-Control
Plasmid#122277PurposeLentiviral vector for stable expression of Firefly luciferase.DepositorInsertFirefly luciferase
UseLentiviral and LuciferaseExpressionBacterialPromoterThymidine kinaseAvailable SinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKD184
Plasmid#90953PurposeExpresses ThsR under the PltetO-1 promoter, sfGFP under PphsA, and mCherry constitutivelyDepositorInsertsThiosulfate response regulator
Superfolder GFP
mCherry
ExpressionBacterialPromoterBba_J23114, PltetO-1, and PphsAAvailable SinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
lenti-Cas9-VQR-Blast
Plasmid#87155Purposelentiviral expression of SpCas9-VQR (NGA PAM restricted)DepositorInsertSpCas9-VQR(D1135V,R1335Q,T1337R)
UseLentiviralMutationD1135V,R1335Q,T1337RAvailable SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTH101
Plasmid#84459PurposepJB38-NWMN29-30 + SarA_P1-DsRed-TermDepositorInsertSarA_P1-DsRED from pTH1
Available SinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSP2262
Plasmid#70703PurposeBacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationD10A & H557A in SaCas9PromoterT7Available SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
HSC1-GiP
Plasmid#58254PurposeThis self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassetteDepositorInsertEnhanced Green Fluorescence Protein, Puromycin resistance gene
UseRetroviralExpressionMammalianPromoterHuman EF1-alphaAvailable SinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-HPV59 L1L2
Plasmid#52586PurposeExpresses HPV59 L1 and L2 in mammalian cellsDepositorInsertsHPV59 L1
HPV59 L2
ExpressionMammalianAvailable SinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-dCas9-P2A-EGFP
Plasmid#188898PurposeHuman lentiviral vector for expression of dCas9 with a C-terminal HA-2xNLS and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening elementDepositorInsertdCas9
UseLentiviralTagsHA-2xNLS and P2A-GFPPromoterSFFVAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
RNA-Protein Landing Pad
Plasmid#226162PurposeUsed to generate a landing pad in Flp-In cells containing promoters for protein and MS2-circRNA barcode expressionDepositorInsertsmTagBFP2-P2A-iCasp9
Reverse tetracycline transactivator 3
Hygromycin-IRES-EGFP
UseSynthetic BiologyTagsHAExpressionMammalianAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_ABE8e-SpRY-P2A-BFP_HygroR
Plasmid#213009PurposeA lentiviral vector used to create stable cell lines expressing the ABE8e-SpRY base editor and BFPDepositorInsertABE8e-SpRY-D10A
UseCRISPR and LentiviralExpressionMammalianMutationD10A nickase variant of SpRYPromoterEf-1aAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.B4
Plasmid#127849Purposenon-standard AAV2 rep-AAV-PHP.B4 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.B4 VP1 gene
UseAAVExpressionMammalianPromoterp41Available SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp MCP-mCherry IRES h2b-diRFP
Plasmid#102351PurposeLentiral expression vector bearing the MS2 Coat Protein (MCP) fused to mCherry as well as the nuclear marker histone 2b fused to two copies of irFP. Also bears the hygromycin selection marker.DepositorInsertMS2 Coat Protein
UseLentiviralTagsmCherryPromoterSFFVAvailable SinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pY036_ATP1A1_G3_Array
Plasmid#86619PurposeVector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmidDepositorInsertATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA
UseCRISPR; Co-selection via nhej using ouabainTags3xHAExpressionMammalianPromoterCBhAvailable SinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-lxlplap
Plasmid#45581DepositorInsertsUseLentiviralTagsHistone 2BExpressionMammalianPromoterUbiquitinAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only