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pUBC_HA_2xPP7-VP64

Plasmid

#68418

Purpose

Transient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter

Depositor

John Rinn

Publication

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

PP7(deltaFG)

Use

Synthetic Biology

Tags

HA and VP64

Expression

Mammalian

Mutation

PP7: [delta]67_74 (non-aggregating)

Promoter

human UbC

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pJFRC173-20XUAS-IVS-Dre::PEST

Plasmid

#32146

Purpose

Expresses Dre recombinase under control of 20XUAS sites in Drosophila

Depositor

Gerald Rubin

Publication

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

Dre::PEST

Expression

Insect

Promoter

Hsp70

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-hATG7CS

Plasmid

#87868

Purpose

Express a active-site mutant human Atg7/Apg7 C572S in mammalian cells

Depositor

Eiki Kominami, Isei Tanida

Publication

Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

Insert

ATG7 C572S mutant (ATG7 Human)

Expression

Mammalian

Mutation

C572S mutant

Promoter

CMV

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV Δ18

Plasmid

#169465

Purpose

Encodes SARS-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Publication

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pSF143-wArchon1-KGC-EGFP-ER2

Plasmid

#108429

Purpose

Codon-optimized Archon1 fluorescent voltage reporter in C.elegans expression plasmid

Depositor

Edward Boyden

Publication

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

wArchon1-KGC-EGFP-ER2

Expression

Worm

Promoter

flp-18

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b LFn-Fla

Plasmid

#84871

Purpose

Bacterial expression of LFn-Fla fusion

Depositor

Russell Vance

Publication

Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

Inserts

LFn
Fla

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pJJH1295

Plasmid

#36914

Depositor

Jürgen Heinisch

Publication

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Insert

3x mCherry

Expression

Bacterial, Mammalian, and Y…

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pT2/CMV-Luc/PGK-SB100X

Plasmid

#192869

Purpose

Carries luciferase under CMV promoter flanked with IR/DR elements. Also carries SB100X under PGK promoter outside of the IR/DR elements.

Depositor

Maria Castro, David Largaespada

Publication

Calinescu et al J Vis Exp. 2015 Feb 22;(96). doi: 10.379

Inserts

luc
SB100X

Expression

Mammalian

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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STAGR_gRNAScaffold_mU6

Plasmid

#102844

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Publication

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert gRNAScaffold_mU6

Use

Pcr template for stagr inserts

Promoter

mU6

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Lenti-sgApc/Cre

Plasmid

#89641

Purpose

Expresses an Apc-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Publication

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Apc (Apc Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pSYC-437

Plasmid

#163829

Purpose

Foxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.

Depositor

Seok-Yong Choi

Publication

Zhang et al Nat Commun. 2020 Apr 20;11(1):1860. doi:

Inserts

Foxj1a Promoter
GCaMP6s

Use

Multisite gateway destination vector

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRosa26-Acceptor (AG103)

Plasmid

#99632

Purpose

To insert the Tre-Tight or UAS ifgMosaic Donor vectors

Depositor

Rui Benedito

Publication

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Gt(Rosa)26Sor (Gt(ROSA)26Sor Mouse)

Use

Mouse Targeting

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSR83

Plasmid

#90211

Purpose

Expresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoter

Depositor

Jeffrey Tabor

Publication

Olson et al Mol Syst Biol. 2017 Apr 24;13(4):926. do

Inserts

OmpR (ompR E. coli)
mCherry

Use

Synthetic Biology

Expression

Bacterial

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9 ABE-Umax

Plasmid

#222138

Purpose

Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Publication

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9 (cas9 Synthetic)

Use

CRISPR; Zebrafish expression

Tags

bipartite NLS

Promoter

T3

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Mammalian expression: Z-lock cofilin dark state mutant

Plasmid

#141136

Purpose

a control for optogenetic cofilin analog

Depositor

Klaus Hahn

Publication

Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

Insert

Zdk2, Cofilin, LOV2

Expression

Mammalian

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pEf1a OsTIR ires NEO pA T2BH

Plasmid

#127910

Purpose

Transposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoter

Depositor

Mark Groudine

Publication

Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

Insert

OsTIR

Use

Transposon

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUG-nLuc-3XFLAG

Plasmid

#127299

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

June 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-blueCKAR

Plasmid

#118472

Purpose

cpBFP-based single-color biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Publication

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

blueCKAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP

Plasmid

#86987

Purpose

For cloning and expression of sgRNA together with expression of Cas9 and TagRFP

Depositor

Ralf Kuehn

Publication

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

Cas9

Expression

Mammalian

Promoter

CAG

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pS1643 (pFA6-NATMX-CDC42pr-yGFP1-10)

Plasmid

#86420

Purpose

N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter

Depositor

Sue Jaspersen

Publication

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

CDC42pr-GFP1-10

Tags

GFP1-10

Expression

Yeast

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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