167,150 results
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Plasmid#140645PurposeCTCF tagging with mAID-CloverDepositorAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only
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pFA6a-link-ytdTomato-URA3
Plasmid#168054PurposeReplaces Addgene plasmid 118461. Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertytdTomato
TagsytdTomatoExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-eLACCO2.1-NGR
Plasmid#208023PurposeBiosensor for extracellular L-lactateDepositorInserteLACCO2.1
ExpressionMammalianAvailable SinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETMCSI-T4P_Y320A
Plasmid#122522PurposeExpression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesisDepositorInsertgp43 - DNA polymerase (43 Escherichia virus T4)
ExpressionBacterialMutationtyrosine 320 changed to alaninePromoterT7 promoterAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-ATXN1
Plasmid#48189PurposeExpresses Ataxin1DepositorAvailable SinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLPCX2-MT1-MMP-eGFP
Plasmid#89819PurposeExpress in MT1-MMP mammalian cellsDepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Cas9_Blast
Plasmid#125592PurposeLentiviral expression of spCas9 with blasticidin resistance geneDepositorInsertCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianPromoterEFS promoterAvailable SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
ADORA2B-Tango
Plasmid#66211PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJC005.em
Plasmid#182738PurposeCRISPR-Cas12a genome editing of E. faeciumDepositorTypeEmpty backboneUseCRISPRAvailable SinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP
Plasmid#66707Purposeexpresses a single gRNA and VP64-dCas9-VP64DepositorInsertshU6-gRNA expression cassette
S.Pyogenes VP64-dCas9-VP64
UseLentiviral and Synthetic BiologyTagsVP64MutationD10A and H840APromoterhU6 and hUbCAvailable SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tn7 helper plasmid
Plasmid#141161PurposeTemperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoterDepositorInsertTn7 transposon machinery
ExpressionBacterialPromoterArabinose-inducible pBADAvailable SinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKH3-TRIM28-C65A/C68A
Plasmid#92199PurposeMammalian overexpression vector for TRIM28; catalytically inactive (mutated on C65A and C68A)DepositorInsertTif1b (TRIM28 Human, Synthetic)
TagsHAExpressionMammalianMutationChanged Cysteines 65 and 68 to Alanines.Available SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-P(Per2)-DIO-intron2-dLUC
Plasmid#110059PurposePer2 transcription luciferase reporterDepositorInsertPer2 promoter and luciferase (Per2 Mouse)
UseAAV and Cre/LoxExpressionMammalianPromoterPer2Available SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
All_in_one_CRISPR/Cas9_LacZ
Plasmid#74293Purpose"All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screeningDepositorInsertsLacZ-alpha
Cas9
mCherry
UseCRISPRTagsHAExpressionBacterial and MammalianPromoterLac promoter, SV40, and T7 promoterAvailable SinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQFmcs-2H12.D11
Plasmid#221183PurposecdGreen2 expressed from a cumate-inducible promoter (Internal Lab ID: UJ11216)DepositorInsertcdGreen2
ExpressionBacterialPromoterPQ5 (cumate-inducible)Available SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLS.400
Plasmid#187201PurposeExpresses retron Eco4 RTDepositorInsertRetron Eco4 Reverse Transcriptase
UseSynthetic BiologyExpressionBacterialPromotermphRAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rich-pT7
Plasmid#108445PurposeThis plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pUC57. Xenopus globin 3UTR and 5UTR, pT7, and polyA added.DepositorTypeEmpty backbonePromoterpT7Available SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO mCherry
Plasmid#114471PurposeFlp-dependent mCherry ControlDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertmCherry
UseAAVPromoterEf1aAvailable SinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDae079 (eMutaT7[transition])
Plasmid#187622PurposeExpresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coliDepositorInsertsTadA-8e-T7RNAP
PmCDA1-T7RNAP
ExpressionBacterialAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_GR-RE_MLPmin_BC1094-luc2
Plasmid#227114PurposeGlucocorticoid receptor response element for luciferase and barcode assays; barcode BC1094DepositorInsert12x clustered GR response element linked to MLP minimal promoter driving barcode 1094 and a luciferase reporter gene
UseAAVExpressionMammalianAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNeonGreen-C1-Nir2-LNS2
Plasmid#220086PurposeC-terminal PA binding domain of Nir2DepositorInsertPITPNM1 (PITPNM1 Human)
TagsNeonGreen-GGSGGMExpressionMammalianMutationamino acids 896-1216PromoterCMVAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSico-shNKCC1
Plasmid#83027Purposeexpresses an shRNA against NKCC1DepositorInsertshNKCC1
UseCre/Lox, Lentiviral, and RNAiExpressionMammalianPromoterU6Available SinceOct. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
AP1muA-TurboID-polyA-G418 HR
Plasmid#227734PurposeHomology repair plasmid for endogenous tagging of AP1muA at the C-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells.DepositorAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_SNAP-tag2
Plasmid#226512PurposeBacterial cell expression of SNAP-tag2DepositorInsertSNAP-tag2
TagsHis-tag (10x) and Strep-Tag II,ExpressionBacterialAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9-P2A-EGFP (RTW3027)
Plasmid#139987PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianPromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV Sport2 mPer2
Plasmid#16204DepositorInsertPer2 (Per2 Mouse)
ExpressionMammalianAvailable SinceDec. 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-YTHDC2
Plasmid#222558PurposePiggyBac transposon plasmid for doxycycline inducible expression of YTHDC2DepositorInsertYTHDC2 (YTHDC2 Human)
ExpressionMammalianAvailable SinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-NEK7
Plasmid#23395DepositorInsertNEK7 (NEK7 Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
TRPML1-G-GECO1.2-ERES
Plasmid#207144PurposeFusion of Ca2+ reporter and lysosomal Ca2+-permeable channel, with ER-export signalDepositorInsertMcoln1 (MCOLN1 Human)
TagsER-Export Signal (ERES) and G-GECO1.2ExpressionMammalianPromoterCMVAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-TRIM28deltaLinker
Plasmid#124958PurposeMammalian expression of Human TRIM28 with deleted Linker region between CC and PHD domainsDepositorInserthTRIM28 (TRIM28 Human)
TagsHAExpressionMammalianMutationDeletion of linker region between CC domain and P…Available SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only