162,160 results

1. pIMAY*

   Plasmid

   #121441

   Purpose

   Allelic exchange plasmid with PheS* counter-selection for Staphylococcus aureus

   Depositor

   Angelika Grundling

   Article

   Schuster et al Microbiology. 2019 Apr 3. doi: 10.1099/m

   Type

   Empty backbone

   Use

   Escherichia coli/staphylococcus aureus shuttle ve…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-VAMP7

   Plasmid

   #45913

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

   Insert

   vesicle-associated membrane protein 7 (Vamp7 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-Rubicon

   Plasmid

   #28022

   Depositor

   Qing Zhong

   Article

   Sun et al J Biol Chem. 2011 Jan 7. 286(1):185-91.

   Insert

   Rubicon (RUBCN Human)
   

   Use

   Tags

   3xFlag and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF2538

   Plasmid

   #144036

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SOX4 (SOX4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)-HA-Flag-natT1R2

   Plasmid

   #113944

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Use

   Tags

   FLAG and Signal/leader sequence from influenza he…

   Expression

   Mammalian

   Mutation

   residues 22-839

   Promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHes1-DsRed

   Plasmid

   #13767

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Hes1 promoter
   

   Use

   Tags

   DsRed2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPP085

   Plasmid

   #158795

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   CasPhi-2
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-EGFP-Cdc42(Q61L)

   Plasmid

   #12600

   Depositor

   Klaus Hahn

   Article

   Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

   Insert

   EGFP-Cdc42 (Q61L) (CDC42 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Q61L

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFUGW-H1 empty vector

   Plasmid

   #25870

   Purpose

   3rd generation lentiviral vector

   Depositor

   Sally Temple

   Article

   Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   Aug. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-DIO-Sun1GFP-WPRE-pA

    Plasmid

    #160141

    Purpose

    Cre- dependent expression of Sun1GFP for nuclei isolation

    Depositor

    Andreas Pfenning

    Article

    Lawler et al J Neurosci. 2020 Nov 10. pii: JNEUROSCI.

    Insert

    Sun1-GFP (Sun1 Mouse)
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N-truncated: removed amino acids 1-207, retained …

    Promoter

    Ef1a

    Available since

    March 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. EF1a- gfp-2a-puro

    Plasmid

    #129443

    Purpose

    expression of GFP and Puro R

    Depositor

    Oskar Laur

    Insert

    gfp-puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMRX-INU-FLAG-CCPG1

    Plasmid

    #128262

    Purpose

    Expresses CCPG1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    CCPG1 (CCPG1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1/CALR wt-FLAG

    Plasmid

    #204532

    Purpose

    Mammalian expression of human CALR wt-FLAG

    Depositor

    Norio Komatsu

    Article

    Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

    Insert

    CALR wt-FLAG (CALR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-HA-mUbp43

    Plasmid

    #12454

    Depositor

    Dong-Er Zhang

    Article

    Malakhova et al EMBO J. 2006 Jun 7. 25(11):2358-67.

    Insert

    UBP43 (Usp18 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5-HA-Parkin

    Plasmid

    #17613

    Depositor

    Ted Dawson

    Article

    Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

    Insert

    Parkin (PRKN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXR004: CasRx pre-gRNA cloning backbone

    Plasmid

    #109054

    Purpose

    hU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUCP20T-eyfp

    Plasmid

    #78466

    Purpose

    Broad range bacterial fluorescence labeling

    Depositor

    Mariette Barbier

    Article

    Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

    Insert

    eYFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-GRAB_NE1m

    Plasmid

    #123308

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Has Service

    AAV Retrograde and AAV9

    Insert

    GPCR activation based NE sensor GRAB_NE1m
    

    Use

    AAV

    Tags

    Expression

    Mutation

    contains a glycine-to-threonine mutation at posit…

    Promoter

    hSyn

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA5/FRT/TO_H2B_HaloTag9_T2A_EGFP

    Plasmid

    #169332

    Purpose

    Nuclear expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag9 (H2BC21 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHEN2-SG4 R105A

    Plasmid

    #196072

    Purpose

    Express mutant (R105A) SG4 in E.coli

    Depositor

    Shankar Balasubramanian

    Article

    Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

    Insert

    SG4 R105A
    

    Use

    Tags

    3XFLAG and 6XHis

    Expression

    Bacterial

    Mutation

    R105A mutation in SG4

    Promoter

    T7

    Available since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDSP33

    Plasmid

    #199375

    Purpose

    Encodes dual cistronic nuclear localized jGCaMP7f and mScarlet-I optimized for C. elegans expression

    Depositor

    Hang Lu

    Insert

    NLS::jGCaMP7f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    no mutations, except added subcellular localizati…

    Promoter

    Available since

    April 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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22. pSMPUW_6.16_ M4_Ef1a-gateway-V5-SV40-Zeo

    Plasmid

    #128452

    Purpose

    lentiviral destination vector with Ef1a promoter, C-terminal V5 tag, SV40 promoter, and Zeo Resistance gene

    Depositor

    Lindy Barrett

    Insert

    Ef1a promoter, C-terminal V5 tag, SV40 promoter, and Zeocin Resistance gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p2T-CMV-ABEmax-BlastR

    Plasmid

    #152989

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    ABE7.10-nSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mCherry-Rab5C-S35N

    Plasmid

    #110503

    Purpose

    Dominant Negative

    Depositor

    Bernardo Mainou

    Insert

    Rab5C-S35N (RAB5C Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    S35N

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. EGFPC2-CB2 SH3+

    Plasmid

    #68821

    Purpose

    EGFP tagged Collybistin expression in mammalian cells

    Depositor

    Shiva Tyagarajan

    Article

    de Groot et al PLoS Genet. 2017 Oct 25;13(10):e1007073.

    Insert

    Collybistin (Arhgef9 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGWB505

    Plasmid

    #74847

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pB-CMPE-ePPEplus

    Plasmid

    #205246

    Purpose

    For plant prime editing in wheat plants

    Depositor

    Zong Yuan

    Article

    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

    Insert

    Csy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Detailed in manuscript

    Promoter

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA5/FRT-HA-hM3D(Gq)

    Plasmid

    #45547

    Purpose

    Expresses hM3D (Gq) neuronal activator

    Depositor

    Bryan Roth

    Article

    Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

    Insert

    hM3D (Gq)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CMV mRuby3-Synapsin1a

    Plasmid

    #187896

    Purpose

    For labeling pre-synaptic terminals

    Depositor

    Timothy Ryan

    Insert

    Synapsin1a
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGL2-Full

    Plasmid

    #16012

    Depositor

    Wafik El-Deiry

    Article

    Takimoto et al Oncogene. 2000 Mar 30. 19(14):1735-43.

    Insert

    KILLER/DR5 (TNFRSF10B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only