164,826 results

1. pGEX-6p1-GST-ArcFL

   Plasmid

   #119877

   Purpose

   Bacterial Expression construct for protein production and purification

   Depositor

   Jason Shepherd

   Article

   Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

   Insert

   Activity Regulated Cytoskeletal Protein (Arc Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGLO-Blue

   Plasmid

   #220782

   Purpose

   Expresses a blue chromoprotein (visible light) with a his-tag and an inducible GFP (araBAD-promoter)

   Depositor

   Angela Leu

   Insert

   blue chromoprotein (aeBlue)
   

   Use

   Tags

   8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21b(+)-Is-PETase-W159H-S238F

   Plasmid

   #112203

   Purpose

   pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12

   Depositor

   Gregg Beckham, Christopher Johnson

   Article

   Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

   Insert

   PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
   

   Use

   Tags

   6XHIS

   Expression

   Bacterial

   Mutation

   W159H, S238F

   Promoter

   N/A

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-Hylight-T152E

   Plasmid

   #193448

   Purpose

   A binding-dead fluorescent biosensor for fructose 1,6-bisphosphate (FBP) for negative control experiments (mammalian expression vector)

   Depositor

   Richard Goodman

   Article

   Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

   Insert

   Binding-Dead Hylight
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Threonine 67 (ACT) mutated to a Glutamic Acid (GA…

   Promoter

   CMV

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX iLOV

   Plasmid

   #26587

   Depositor

   John Christie

   Article

   Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 1

   Insert

   iLOV
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. ClopHensorN

   Plasmid

   #50758

   Purpose

   Mammalian expression vector expressing the ClopHensorN chloride and proton sensor

   Depositor

   Colin Akerman

   Article

   Raimondo et al Front Cell Neurosci. 2013 Nov 13;7:202.

   Insert

   ClopHensorN
   

   Use

   Tags

   Strep-II

   Expression

   Mammalian

   Mutation

   E2GFP is EGFP-T203Y

   Promoter

   CAG

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-hALIX

   Plasmid

   #21504

   Depositor

   James Hurley

   Article

   Lee et al Science. 2008 Oct 24. 322(5901):576-80.

   Insert

   ALIX (PDCD6IP Human)
   

   Use

   Tags

   Cherry

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available Since

   Sept. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. CAG-BFP-sesRNA-GFP-WPRE-pA

   Plasmid

   #192063

   Purpose

   Expression of BFP, sesRNA in mammalian cells, with GFP as efRNA.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for tdTom
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-UbC-Tet1c-dCas9

   Plasmid

   #232178

   Purpose

   Lentiviral cassette that expresses Tet1c-dCas9 fusion with a short linker in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

   Insert

   Tet1 catalytic domain - dead Cas9 - 2A - BlastR (TET1 Human, S. pyogenes)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   hUbC

   Available Since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-Paxillin-22

    Plasmid

    #55114

    Purpose

    Localization: Focal Adhesions, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Article

    Paszek et al Nat Methods. 2012 Jul 1;9(8):825-7. doi:

    Insert

    Paxillin
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. phDLX2-N174

    Plasmid

    #60860

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses DLX2 under the EF1a promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    DLX2 (DLX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-PuroR-SMAR-g5

    Plasmid

    #208377

    Purpose

    Episomal plasmid for expression of 2 proteins with puromycin selection and EGFP monitoring

    Depositor

    Daniel Hawiger

    Article

    Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human CMV

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-pTH-iCre:EGFP-WPREpA

    Plasmid

    #213142

    Purpose

    Truncated rat TH promoter expressing iCre fused to EGFP

    Depositor

    Ulrik Gether

    Article

    Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

    Insert

    iCre
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Truncated TH promoter from rat (Rattus norvegicus…

    Available Since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. L13-Arl13bGFP

    Plasmid

    #40879

    Purpose

    3rd generation transfer vector

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. R777-E172 Hs.PIK3R1-nostop

    Plasmid

    #70456

    Purpose

    Gateway ORF clone of human PIK3R1 [NM_181523.2] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PIK3R1 (PIK3R1 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPlacPbuCas13b-gRNA-T-deGFP

    Plasmid

    #184840

    Purpose

    Expression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    single-spacer CRISPR array with spacer #1 targeting the the mRNA of deGFP, PbuCas13b nuclease
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 and lac promoter

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD24-sfGFPx1

    Plasmid

    #51558

    Purpose

    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli

    Depositor

    Francisco Malagon, Sankar Adhya

    Article

    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

    Insert

    superfolder GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-10nm-EBFP2

    Plasmid

    #231184

    Purpose

    Expresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 10 nm

    Depositor

    Dmitry Lim

    Article

    Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

    Insert

    10nm-EBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB-CAG-dCas9-VPR-pgk-hph

    Plasmid

    #233066

    Purpose

    Piggybac CRISPRa construct

    Depositor

    Markus Landthaler

    Article

    Vučićević et al bioRxiv 2024.09.12.612198

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRRL-SV40-Puro-CMV-mCherry-EGFP-LC3B

    Plasmid

    #223712

    Purpose

    Expresses autophagy flux LC3B fluorescence reporter

    Depositor

    Benjamin Wolozin

    Article

    Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

    Insert

    LC3B (MAP1LC3B Synthetic, Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    mCherry::EGFP::LC3B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. Flag-GFP-TurboID-NES_pLX304

    Plasmid

    #202019

    Purpose

    expresses GFP-TurboID in the mammalian cytosol, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    Flag-GFP-TurboID-NES
    

    Use

    Lentiviral

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pRK-ATF4

    Plasmid

    #26114

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF4 (ATF4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Mito-PyronicSF/pCMV-myc-mito

    Plasmid

    #124813

    Purpose

    Highly resposive GFP-based pyruvate nanosensor to explore mitochondrial metabolism.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Insert

    PyronicSF
    

    Use

    Tags

    Mitochondrial Sequence Signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pBzCas13b-gRNA-NT

    Plasmid

    #164861

    Purpose

    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for BzCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    BzCas13b-gRNA-NT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBzCas13b-gRNA-1

    Plasmid

    #164858

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    BzCas13b-gRNA-1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. SpCas9-CBE6b-IVT

    Plasmid

    #215834

    Purpose

    Template for in vitro transcription of CBE6b (with SpCas9)

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6b-SpCas9-2xUGI
    

    Use

    In vitro transcription template

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. pCDF-Frb-v1

    Plasmid

    #201923

    Purpose

    E. coli vector expressing FrbA, FrbB, FrbC, FrbD and FrbE for the biosynthesis of non-hydrolyzable phosphoserine from phosphoenolpyruvate.

    Depositor

    Ryan Mehl, Richard Cooley

    Article

    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

    Inserts

    FrbA
    FrbB
    FrbC
    FrbD
    FrbE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (modified), T7 (modified, moderate), T7 (modif…

    Available Since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

    Plasmid

    #138151

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    hU6_Rfxcas13d-DR_BsmBI
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti-EFS-MCS-WPRE

    Plasmid

    #163362

    Purpose

    The empty pLenti backbone with multiple cloning site that allows shuttling between pMY and pMX vectors

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCRISPR-HOT-mNeon-P2A-T2A-mCherry-CAAX

    Plasmid

    #240799

    Purpose

    CRISPR-HOT donor plasmid for mNeonGreen fusion protein co-expressing membrane-bound mCherry via a P2A-T2A tandem

    Depositor

    Hans Clevers

    Article

    Krueger et al Science. 2025 Sep 4;389(6764):eadr8753.

    Insert

    mNeonGreen-mCherry-CAAX
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge