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plenti-UBC-Myc-gate-pGK-HYG

Plasmid

#107395

Purpose

Lentiviral gateway destination vector

Depositor

William Kaelin

Publication

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Tags

Myc

Expression

Mammalian

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-APEX2

Plasmid

#108894

Purpose

Multisite gateway vector for 3' tagging with APEX2

Depositor

Rob Parton

Publication

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

APEX2

Use

Gateway Cloning

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGL3-U6-sgRNA-BFP

Plasmid

#107722

Purpose

For expression of sgRNA from the U6 promoter with BFP expression simultaneously

Depositor

Xingxu Huang

Publication

Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

Type

Empty backbone

Use

CRISPR

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pSMART-SOS-GFPuv

Plasmid

#102283

Purpose

Fluorescent sensor of E. coli SOS response

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

cda-GFPuv

Expression

Bacterial

Promoter

cda SOS promoter

Available since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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iRFP-Sec61beta

Plasmid

#108125

Purpose

ER marker

Depositor

Gerry Hammond

Publication

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

iRFP:SEC61B (SEC61B Human)

Expression

Mammalian

Promoter

CMV

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_T5-ARG1

Plasmid

#106476

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917

Depositor

Mikhail Shapiro

Publication

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG1

Use

Synthetic Biology

Expression

Bacterial

Promoter

T5

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBBR8k-GFPuv

Plasmid

#106385

Purpose

L-arabinose-inducible araBAD promoter expressing gfpuv on BBR1 origin

Depositor

Brian Pfleger

Publication

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

araBAD

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-GFP1

Plasmid

#106408

Purpose

P-RAN-GFP1 (RANbody GFP1::vHRP)

Depositor

Joshua Sanes

Publication

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

P-RAN-GFP1

Tags

HA and His

Expression

Mammalian

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEF5-FRT-V5-DEST-GFP-Kif26b

Plasmid

#102862

Purpose

Expresses GFP-Kif26b in mammalian cells

Depositor

Henry Ho

Publication

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Mouse Kif26b (Kif26b Mouse)

Tags

GFP

Expression

Mammalian

Promoter

EF1A

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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dCas9-FOG1[N+C]

Plasmid

#100085

Purpose

dCas9 fused to two FOG1 peptide [aa 1-45] , one at the N-terminus and one at the C-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Publication

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FOG1(aa 1-45) (ZFPM1 Human)

Use

CRISPR

Tags

3XFLag-NLS-FOG1-dCas9-FOG1-NLS

Expression

Mammalian

Promoter

CMV

Available since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAD-LyseR (in lacZ deficient strain)

Plasmid

#99245

Purpose

For autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

Depositor

Jeff Hasty

Publication

Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

Insert

phage lambda gene R (R phage lambda)

Expression

Bacterial

Promoter

beta-lactamase promoter

Available since

Oct. 14, 2017

Availability

Academic Institutions and Nonprofits only

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AAEL010097-Cas9

Plasmid

#100707

Purpose

The promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

Depositor

Omar Akbari

Publication

Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

Inserts

AAEL010097-hspcas9-GFP
Opie2-dsRed

Use

CRISPR

Tags

GFP and dsRED

Expression

Insect

Promoter

AAEL010097 and Opie2

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLKO.1-TRC.mKO2

Plasmid

#85208

Purpose

Backbone for lentiviral gene silencing with monomeric Kusabira-Orange2 expression

Depositor

Sandra Martha Gomes Dias

Publication

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV_hsyn_NES-his-CaMPARI2-F391W-G395D-WPRE-SV40

Plasmid

#101063

Purpose

AAV vector expressing CaMPARI2_F391W-G395D (Kd = 530nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Publication

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Insert

CaMPARI2_F391W-G395D

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSAM2_mCherry_Gata4

Plasmid

#72690

Purpose

Doxycyline regulated expression of Gata4 in mammalian cells

Depositor

Timothy Kamp

Publication

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Gata 4 (Gata4 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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IQGAP1_pLX307

Plasmid

#98346

Purpose

Lentiviral expression of IQGAP1

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

IQGAP1 (IQGAP1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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sox10:mCherry

Plasmid

#98695

Purpose

I-SceI meganuclease-mediated mCherry transgenesis in zebrafish

Depositor

Thomas Look

Publication

Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

Insert

mCherry

Use

Zebrafish expression

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pFREE_Amp

Plasmid

#92051

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Publication

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Cas9
gRNA array

Use

CRISPR

Promoter

pRhamBAD and ptet

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pMonAID_nCas9-PmCDA_Hyg_ALS

Plasmid

#91693

Purpose

Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls

Depositor

Akihiko Kondo

Publication

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

2x35S

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pRRLsinPGK_IRFP720_WPRE

Plasmid

#91785

Purpose

Constitutive expression of IRFP720 using PGK promoter in a 3rd generation lentiviral backbone.

Depositor

Beverly Torok-Storb

Insert

iRFP720

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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