161,123 results

1. pZR071_SFFV-dCas9-mCherry-KRAB

   Plasmid

   #180264

   Purpose

   Lentiviral expression vector for dCas9-KRAB in mammalian cells

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   KRAB and mCherry

   Expression

   Mammalian

   Mutation

   Nuclease dead Cas9

   Promoter

   SFFV

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSG5-HA-p300

   Plasmid

   #89094

   Purpose

   mammalian expression of full-length human p300 in pSG5

   Depositor

   Elizabeth Wilson

   Article

   Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

   Insert

   p300 (EP300 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.4_StrepII_TEV_PP2Ac

   Plasmid

   #217477

   Purpose

   Expresses PP2Ac with a TEV cleavable N-terminal StrepII tag

   Depositor

   Wolfgang Peti

   Article

   Padi et al Nature. 2024 Jan;625(7993):195-203. doi:

   Insert

   PP2Ac
   

   Use

   Tags

   StrepII, TEV

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETM6

   Plasmid

   #49795

   Purpose

   ePathBrick vector for pathway engineering in E. coli.

   Depositor

   Mattheos Koffas

   Article

   Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

   Type

   Empty backbone

   Use

   Tags

   S-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-VEGFR1-V5/HIS

   Plasmid

   #205616

   Purpose

   Expression of human VEGFR1 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   VEGFR1 receptor tyrosine kinase (FLT1 Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

   Plasmid

   #185919

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-MCS-TurboID-3xFLAG

   Plasmid

   #186814

   Purpose

   CAG promoter-driven expression of BioID2-fused protein (C terminus)

   Depositor

   Masahito Ikawa

   Article

   Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

   Type

   Empty backbone

   Use

   Tags

   TurboID-3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLv-HSA-uDys/eGFP

   Plasmid

   #26810

   Depositor

   Jeffrey Chamberlain

   Article

   Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

   Insert

   dystrophin (DMD Human)
   

   Use

   Lentiviral; Lentiviral plasmid backbone from l. n…

   Tags

   eGFP

   Expression

   Mutation

   deleted R4-R23 deleted exons 71-78

   Promoter

   Available since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMyBADC-kan

   Plasmid

   #84689

   Purpose

   Protein expression in mycobacteria

   Depositor

   Matthias Wilmanns

   Article

   Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   AraC

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET StrepII TEV LIC cloning vector (1R)

    Plasmid

    #29664

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Strep II and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. FHRE-Luc

    Plasmid

    #1789

    Purpose

    Reporter plasmid for FOXO3a. Forkhead responsive element with luciferase readout.

    Depositor

    Michael Greenberg

    Article

    Brunet et al Cell 1999 Mar 19;96(6):857-68.

    Insert

    FHRE (FOXO3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    3 copies of FRE

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFH2.2_mCherry-MS2x12

    Plasmid

    #205521

    Purpose

    Express mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cyto-V5-APEX2

    Plasmid

    #170572

    Purpose

    Expresses APEX2 in cytosol

    Depositor

    James Olzmann

    Article

    Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

    Insert

    APEX2
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET C-terminal TEV His6 cloning vector with BioBrick polycistronic restriction sites (9Bc)

    Plasmid

    #48285

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-CD81-(TEV)-FKBP-SmBiT

    Plasmid

    #182651

    Purpose

    human CD81-TEV cleavage site-FKBP-SmBiT tag

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

    Insert

    human CD81-TEVp cleavage site-FKBP-SmBiT (CD81 Human, Synthetic)
    

    Use

    Tags

    SmBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR_Gal4UAS_Tbet_T2A_GFP_PGK_mCherry

    Plasmid

    #85431

    Purpose

    Lentiviral vector for Gal4 inducible Tbet T2A GFP and constitutive mCherry expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    human Tbet
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1 hygro shRNA E-cadherin

    Plasmid

    #18802

    Depositor

    Bob Weinberg

    Article

    Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

    Insert

    E-cadherin shRNA (CDH1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCI-MS2V5-PABPC1

    Plasmid

    #65807

    Purpose

    expression of PABPC1-MS2 fusion. MS2 tethers PABPC1 to mRNAs containing MS2 stem loops.

    Depositor

    Niels Gehring

    Article

    Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

    Insert

    PAB1 (PABPC1 Human)
    

    Use

    Tags

    MS2 stem loops and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. gag-MCP-pol

    Plasmid

    #211370

    Purpose

    Packaging plasmid for v3 PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    MMLVgag-MCP-pol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. CXCR7-DuET

    Plasmid

    #213224

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CXCR7 (ACKR3 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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