We narrowed to 24,865 results for: nes
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Plasmid#82581PurposeThe plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNADepositorInsertCas9
ExpressionBacterialPromoterT7Available SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Tat K41A HA (P#37)
Plasmid#14655DepositorAvailable SinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 -5'cMyc-SMCl- S957A
Plasmid#32368DepositorAvailable SinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pICSL50015
Plasmid#50318PurposePlease note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)DepositorInsertCDS, mNEON (Branchistoma lanceolatum)
UsePlant expressionMutationBsaI/BbsI sites removed by point-mutationAvailable SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 5' cMyc- SMCI-S966A
Plasmid#32371DepositorAvailable SinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
Nkx6.2
Plasmid#15542DepositorAvailable SinceAug. 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_cys_his
Plasmid#210403PurposeFor expression of VHH with C-terminal cysteine and his tagDepositorTypeEmpty backboneTagscys-his6ExpressionBacterialPromoterlacAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_sortase_his
Plasmid#210406PurposeFor expression of VHH with C-terminal sortase and his tagsDepositorTypeEmpty backboneTagssortase-his6ExpressionBacterialPromoterlacAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_his_myc
Plasmid#210526PurposeFor expression of VHH with C-terminal his and myc tagsDepositorTypeEmpty backboneTagsHis6-mycExpressionBacterialPromoterlacAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKSB-611U6-sgRNA3 AGAG AACA
Plasmid#173673PurposeTo clone and express a gRNA in AnophelesDepositorTypeEmpty backboneUseCRISPRExpressionInsectAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pK3F
Plasmid#85770PurposeN-ICE plasmid pK3FDepositorInsertloxP-KanMX-loxP-3FLAG
UseCre/LoxExpressionYeastAvailable SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pH3FS-GAL1
Plasmid#85782PurposeN-ICE plasmid pH3FS-GAL1DepositorInsertloxP-HphMX-loxP-GAL1p-3FLAG
UseCre/LoxExpressionYeastAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
DLC1A
Plasmid#25333PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertDLC1:S1074-L1283 (DLC1 Human)
TagsHisExpressionBacterialMutationContains amino acids S1074-L1283Available SinceAug. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCRUSH
Plasmid#54480PurposeMouse genomic ROSA26 targeting vector, expresses EGFP, cre/lox conditional U6 promoter pgk-puroDepositorTypeEmpty backboneUseCre/Lox, Mouse Targeting, and RNAiTagsSA-EGFP-polyA, U6 promoter, loxP, and pgk-puroExpressionMammalianPromoterU6Available SinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pISH-Tshz1
Plasmid#74360PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Tshz1. Not for gene expression.DepositorInsertteashirt zinc finger family member 1 (Tshz1 Mouse)
Available SinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCF730
Plasmid#121668PurposeEF1a-ProCas9-Flavi-P2A-PuroDepositorInsertCas9(C)
UseLentiviralPromoterEF-1a core promoterAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBDEST.N
Plasmid#79468PurposePromoterless, Sleeping Beauty-compatible Gateway destination vector, NeomycinDepositorTypeEmpty backboneUsePromoterless gateway destination vectorPromoternoneAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Hy_pUbi-p63e eGFP SV40
Plasmid#132650PurposeExpresses eGFP mRNA from the Drosophila Ubi-p63e promoterDepositorInserteGFP
ExpressionInsectPromoterUbi-p63eAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
3XFlag-pLPC
Plasmid#73560Purposevector encoding 3 Flag tagsDepositorInsert3X-Flag
UseRetroviralTags3X-FLAGExpressionMammalianPromoterCMVAvailable SinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA3
Plasmid#199729PurposeGolden Gate entry vector; 3rd gRNA under TaU3 promoterDepositorInsertTaU3-sgRNA3 scaffold
ExpressionPlantAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA4
Plasmid#199730PurposeGolden Gate entry vector; 4th gRNA under ZmU6-C1 promoterDepositorInsertZmU6C1-sgRNA4 scaffold
ExpressionPlantAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA5
Plasmid#199731PurposeGolden Gate entry vector; 5th gRNA under ZmU6-C3 promoterDepositorInsertZmU6C3-sgRNA5 scaffold
ExpressionPlantAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA6
Plasmid#199732PurposeGolden Gate entry vector; 6th gRNA under OsU6.1 promoterDepositorInsertOsU6.1-sgRNA6 scaffold
ExpressionPlantAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA7
Plasmid#199733PurposeGolden Gate entry vector; 7th gRNA under OsU6.2 promoterDepositorInsertOsU6.2-sgRNA7 scaffold
ExpressionPlantAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA8
Plasmid#199734PurposeGolden Gate entry vector; 8th gRNA under TaU6 promoterDepositorInsertTaU6-sgRNA8 scaffold
ExpressionPlantAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJC225
Plasmid#176883PurposeMoClo Level1 plasmid, allows transition into Cermak, et al., 2017 GG cloning systemDepositorTypeEmpty backboneUseCloningAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A5803
Plasmid#176878PurposeHigh constitutive expression of Firefly Luciferase in plantsDepositorInsertCmYLCV:Luc+:HSP
UseLuciferaseAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-AtUbi10-6
Plasmid#176871PurposeMoClo Level 0 plasmid, contains AtUbi1 promoterDepositorInsertAtUbi10
ExpressionPlantMutationWTAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTAG2E
Plasmid#176865PurposeFirst sgRNA in a tRNA separated array of sgRNAsDepositorInsertsgRNA_2E
UseCRISPRMutationWTAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMLC-BB1
Plasmid#166922PurposeBackbone for assembly of multi-level gene expression controllersDepositorInsertCyOFP - Orange fluorescent protein - flanked by BsaI sites
UseSynthetic BiologyExpressionBacterialPromoterJ23100 constitutiveAvailable SinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ac5-STABLE1-puro
Plasmid#208031PurposeEnables expression of an ORF (with or without tags) and selection with puromycin in insect cells. Includes GFP-T2A-puro with Actin5C promoter.DepositorTypeEmpty backboneExpressionInsectAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-PIG-miR-30b
Plasmid#64232PurposeExpresses miR-30b in mammalian cellsDepositorAvailable SinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBD21_037
Plasmid#109154PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of iRFP720DepositorInsertsmRFP
iRFP720
UseSynthetic BiologyExpressionBacterial and MammalianPromoterCMV and pTetAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0013
Plasmid#117521PurposeLevel0 Golden Gate part: CDS, SpCas9-pDepositorInsertSpCas9-p (with stop codon)
UseCRISPRAvailable SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLO103
Plasmid#90223PurposeComplements mutations in Aspergillus nidulans gene pyrG (AN6157) (mutants require uridine and uracil)DepositorInsertATET_09675
UseAspergillusPromoterpLacAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTHS
Plasmid#166938PurposeToehold switch multi-level gene expression controller (MLC)DepositorInsertGenetic circuit expressing GFP- Toehold switch
UseSynthetic BiologyExpressionBacterialPromoterPTACAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNH082
Plasmid#42165DepositorInsertmTFP1
ExpressionWormAvailable SinceFeb. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-chi28TAG
Plasmid#188984PurposeExpress chimadanin with TAG at 28 positionDepositorInsertChimadanin with TAG at 28 position
ExpressionBacterialAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
p817-mgammaFcry-EGFP
Plasmid#23156DepositorInsertmouse gammaF crystallin promoter:EGFP (eGFP Mouse, mouse gammaF promoter:EGFP)
UseMulticloning site flanked by i-scei sitesMutationmouse gammaF crystallin promoter (-225 - +73) preā¦Available SinceApril 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RNF17L
Plasmid#35413DepositorAvailable SinceFeb. 25, 2013AvailabilityAcademic Institutions and Nonprofits only