We narrowed to 31,507 results for: REP
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Plasmid#141191PurposeExpresses GFP-ZNF598 in mammalian cells, can be used to make inducible cell lineDepositorAvailable SinceJune 18, 2020AvailabilityAcademic Institutions and Nonprofits only
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pQ39-VC
Plasmid#175061Purposeexpresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ39) in mammalian cellsDepositorInsertpolyQ39 (HTT Human)
TagsmVenus + mCherryExpressionMammalianMutationmVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…PromoterCMV PromoterAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-NRG1-ScNeo
Plasmid#209906PurposeTo monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG eGFP NLS
Plasmid#170830PurposeDonor plasmid for knocking eGFP NLS into AAVS1 safe harbor locusDepositorInserteGFP
UseCRISPR, Synthetic Biology, and TALENTagsNLSExpressionMammalianPromoterCAGAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMy-MouseCTCFbiotag-T2A-mOrange
Plasmid#50564PurposeRetroviral vector endoding for mOrange and mouse CTCF with a short biotinylation substrate (biotag, Kimetal.,2009) fused to its C-termDepositorInsertsAmpicilin
Mouse CTCF Exons
UseRetroviralAvailable SinceFeb. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
LAMP-mChF-AIP
Plasmid#61524PurposeExpression of Homo sapiens lysosomal-associated membrane protein 1 (LAMP1) tagged with mCherry and AIPDepositorInsertlysosomal-associated membrane protein 1 (LAMP1 Human)
TagsAIP and mCherryExpressionMammalianPromoterCMVAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-P3-IgKL-mNG
Plasmid#183465PurposeTo produce AAV with the P3 promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)DepositorInsertIgKL-mNeonGreen
UseAAVTagsIgKL secretory signalAvailable SinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-GFI1B
Plasmid#125597Purposedoxycycline-inducible expression of human GFI1B in mammalian cellsDepositorInsertGrowth Factor Independent 1B transcriptional repressor (GFI1B Human)
UseLentiviralTagsNoneExpressionMammalianPromoterTRE-mCMVAvailable SinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-IL6.Pro
Plasmid#79491PurposeMultiSite Gateway entry clones, first fragment, (attL1, attR5) Human IL6 promoterDepositorAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-LHX8-T2A-PuroR
Plasmid#162350PurposeLentiviral expression of LHX8 under the control of the TetON promoterDepositorAvailable SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX_TRC311 ETV4-S
Plasmid#74983PurposeConstitutive ETV4 ORF, blasticidin selectionDepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCD5-H5_Vietnam_1203/04_sfGFP_ST
Plasmid#182546PurposeExpresses a sfGFP fused H5 protein of the prototype A/Vietnam/1203/04 virusDepositorInsertH5_Vietnam_1203_04
TagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianPromoterCMVAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-ChR2-YFP
Plasmid#204345PurposePiggyBac transposon vector suitable for inserting the ChR2-YFP optogenetic actuator driven by the ubiquitous CAG promoter into the genome of mammalian cell lines.DepositorInsertChR2-YFP
TagsYellow fluorescent proteinExpressionMammalianMutationC-terminus (aa315-737) is deleted - PMID: 1461559…PromoterCAGAvailable SinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT/DEST-3xHA/mGli2 P1-6A
Plasmid#51251PurposeEncodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848,923,956 mutated to AlaDepositorInsertGli2 (Gli2 Mouse)
UseFlpin systemTags3xHA-TEVExpressionMammalianMutationamino acids 789,805,817,848,923,956 mutated to AlaPromoterEF1aAvailable SinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-Ku70-siR-WT
Plasmid#82329PurposePlasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-LivePAR(Y107A)-Hygro
Plasmid#176073PurposeEGFP fused to the C-terminus of a WWE domain containing the mutation Tyr107Ala & a hygromycin resistance cassetteDepositorInsertLivePAR (Y107A)
UseLentiviralTagsEGFPExpressionMammalianMutationPoint mutation to convert Try107 to AlaPromoterEF1AAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
R711-X12-566: His6-MBP-tev-Hs.NF1iso2 (1203-1530)
Plasmid#159580PurposeE. coli protein expression of His6-MBP-tev-Hs.NF1iso2 (1203-1530)DepositorAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-BB0323-ss
Plasmid#137067PurposeE. coli expression clone (T7lac promoter) for mature BB0323 (aa 20-377) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66700.1
TagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature BB0323: lacks the BB0323 signal peptidePromoterT7lacAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-LivePARBackbone
Plasmid#176526PurposeExpression vector with a BamHI site in-frame with a Gly-Ser linker fused to EGFP; serves as the backbone for PAR binding domain incorporation for LivePARDepositorTypeEmpty backboneUseLentiviralTagsEGFPExpressionMammalianPromoterEF1AAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-EF1.Pro
Plasmid#79488PurposeMultiSite Gateway entry clones, first fragment, (attL1, attR5) Human EF1 promoterDepositorInsertEF1 Promoter (EEF1A1 Human, Synthetic)
UseMultisite gateway entry clonePromoterEF1 PromoterAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only