We narrowed to 10,951 results for: T7
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Plasmid#214835PurposeThis plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertNon tagged Avidin
ExpressionBacterialPromoterT7Available SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29b-dspB(E184Q)-6xHis
Plasmid#175802PurposePlasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.DepositorInsertDispersin B
TagsHexahistidine tagExpressionBacterialMutationE184QPromoterT7Available SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
CL7-LbCas12a
Plasmid#164658PurposeBacterial expression of LbCas12a using CL7/Im7DepositorInsertLbCas12a
TagsThioredoxin, CL7, SUMOExpressionBacterialPromoterT7Available SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX - T7pro - EBFP
Plasmid#138615PurposeExpress EBFP with an upstream T7 promoter in a lentiviral backboneDepositorInsertEBFP
UseLentiviralAvailable SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
4xNLS-pMJ915v2-sfGFP
Plasmid#88921PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRExpressionBacterialAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET20b-GpNLuc-6xHis
Plasmid#135932PurposeExpresses the GpNLuc LumiFluor in bacterial cells.DepositorInsertGpNLuc LumiFluor
Tags6xHisExpressionBacterialPromoterT7Available SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLS.601
Plasmid#184955PurposeExpress -Eco1 ncRNA library variantsDepositorInsertEco1 ncRNA (variants)
ExpressionBacterialMutationLibrary members with mutations described in manus…PromoterT7/lacAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oHept
Plasmid#124671PurposeExpresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coilDepositorInsertSpyCatcher002-oHept
TagsC-tag, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP
Plasmid#128424PurposeHuman Codon Optimized dCas9-DNMT3A3LDepositorInsertdCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP
UseCRISPR and LentiviralTagsGFPExpressionMammalianPromoterT7 PromoterAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21-HIS-Tol2
Plasmid#112486Purposefunctional recombinant Tol2 protein from E.coliDepositorAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_TIA1
Plasmid#106095Purposebacterial protein expression of TIA1DepositorAvailable SinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AviTag-SnoopLigase
Plasmid#105626PurposePlasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylationDepositorInsertAviTag-SnoopLigase
ExpressionBacterialPromoterT7Available SinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
S48I48 ELP
Plasmid#68394PurposeElastin-like polypeptide (VPGxG) with 48 repeats of serine guest residue followed by 48 repeats of isoleucine guest residueDepositorInsertS48I48 ELP
ExpressionBacterialPromoterT7Available SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 GFPNanobody_SNAP
Plasmid#82711PurposeExpresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.DepositorInsertGFP Nanobody
Tags6xHis Purification tag, FLAG purification tag, an…ExpressionBacterial and InsectPromoterT7 PromoterAvailable SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+30)
Plasmid#199164Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+30)
TagsMGHHHHHHGGAMutationE16R, V21K, D29K, E42K, E44K, D86K, E100K, D112K,…PromoterT7Available SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMEX174-hCALML3
Plasmid#47600DepositorAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET32-sLatherin
Plasmid#61685Purposebacterial expression of the horse sweat/saliva protein Latherin as a His-tagged thioredoxin fusionDepositorInsertLatherin (LATH horse)
TagsHIS and Trx-Tag thioredoxin proteinExpressionBacterialMutationCodon optimized for E.coliPromoterT7Available SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSL103-SpCas9-FLAG-3XNLS
Plasmid#182032PurposeExpression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNPDepositorInsertStreptococcus pyogenes cas9 (cas9 Synthetic)
UseCRISPRTags12X His tag, 3XNLS, FLAG, and MBPExpressionBacterialPromoterT7Available SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPLV_C1
Plasmid#186415PurposeEndogenous murine RNase inhibitor production for cell-free lysateDepositorInsertMurine RNase Inhibitor
UseSynthetic BiologyPromoterT7Available SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBroad-T7RNAP-apraR
Plasmid#199993PurposeT7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) apraADepositorInsertT7RNAP Circuit
ExpressionBacterialMutationcopFnullAvailable SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only