We narrowed to 9,318 results for: control

1. pAAV-CaMKII-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

   Plasmid

   #165493

   Purpose

   Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

   Depositor

   Thomas Oertner

   Article

   Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

   Insert

   2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
   

   Use

   AAV

   Tags

   darkVenus

   Expression

   Mammalian

   Promoter

   CaMKII

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. INCTbiosyn-pAct-AtINT13

   Plasmid

   #127534

   Purpose

   Plasmid encodes A. thaliana codon optimized Integrase 13.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   Integrase 13 coding sequence codon optimized for A. thaliana expression.
   

   Expression

   Plant

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pROF441

   Plasmid

   #155336

   Purpose

   Binary vector for expression of a gRNA targeting AtAP1 promoter 

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   LB_PAtU6-26-gRNA(PAtAP1)-sgRNA_RB
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-HA-RIPK3 4KR

   Plasmid

   #78813

   Purpose

   To overexpress RIPK3 4KR in Mammalian Cell

   Depositor

   Jaewhan Song

   Article

   Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

   Insert

   RIPK3 (RIPK3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K55R, K89R, K363R, K501R

   Promoter

   CMV

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRRLNeo-pEF1a-p53ashL344P-mCerulean

   Plasmid

   #69581

   Purpose

   Expresses p53-L344P mutant tagged with mCerulean

   Depositor

   Galit Lahav

   Article

   Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

   Insert

   p53-L344P (TP53 Human)
   

   Use

   Lentiviral

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   p53 L344P mutation that prevents oliogomerization…

   Promoter

   EF1alpha

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMT[Hygro]_GBP-3xFlag-DEST

   Plasmid

   #157813

   Purpose

   A gateway plasmid for expression of GBP-3xFlag-fusion protein under the regulation of the copper-inducible metallothionein promoter in Drosophila cells; suitable for hygromycin selection

   Depositor

   Zoltan Lipinszki

   Article

   Torras-Llort et al Commun Biol. 2020 Aug 19;3(1):454. doi:

   Insert

   GFP-binding protein
   

   Use

   For drosophila cells

   Tags

   3xFlag

   Expression

   Insect

   Promoter

   metallothionein

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-NLC-DHFR-SpCas9

   Plasmid

   #124523

   Purpose

   Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

   Insert

   NLC-DHFR-SpCas9
   

   Use

   AAV

   Tags

   Destabilized domain of E.coli dihydrofolate reduc…

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. LightR-bRaf-mVenus

   Plasmid

   #162154

   Purpose

   Expresses LightR-bRaf-mVenus in mammalian cells

   Depositor

   Andrei Karginov

   Article

   Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

   Insert

   LightR-bRaf-mVenus
   

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Valine 600 is substituted with Glutamic acid

   Promoter

   CMV

   Available Since

   Dec. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXs-hs-3xHA-delta6xNHL-LIN-41

   Plasmid

   #52722

   Purpose

   retroviral expression of human LIN-41 / TRIM71 containing deletion of the 6xNHL repeats and 3 N-terminal HA tags

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   LIN-41 (TRIM71 Human)
   

   Use

   Retroviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   deleted amino acids 593–868

   Available Since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRRLHygro-pEF1a-p53ashL344P-mKate2-splitmVenusC

    Plasmid

    #69587

    Purpose

    Expresses mutated p53-L344P tagged with mKate2 and split C-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53-L344P (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - C term

    Expression

    Mammalian

    Mutation

    p53 L344P mutation that prevents dimerization and…

    Promoter

    EF1alpha

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV-Flag-Zfp423-deltaSBD

    Plasmid

    #35971

    Depositor

    Bruce Spiegelman

    Article

    Gupta et al Nature. 2010 Mar 25;464(7288):619-23. do

    Insert

    Zinc Finger Protein 423 (Zfp423 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    lacks zinc fingers 14-19 (aa 640 -838) (delta SMA…

    Available Since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

12. INCTbiosyn-pAct-AtINTBxb1

    Plasmid

    #127536

    Purpose

    Plasmid encodes A. thaliana codon optimized Integrase Bxb1.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase Bxb1 coding sequence codon optimized for A. thaliana expression.
    

    Expression

    Plant

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBMN-puro-P2A-FRB-Fis1

    Plasmid

    #135297

    Purpose

    stably express FBR-Fis1 in mammalian cells with puromycin selection

    Depositor

    Richard Youle

    Article

    Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

    Insert

    FRB-Fis1 (FIS1 Human)
    

    Use

    Retroviral

    Tags

    FRB

    Expression

    Mammalian

    Mutation

    Fis1 tail (aa 93-152)

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-HA-RIPK1 K115R

    Plasmid

    #78835

    Purpose

    To overexpress RIPK1 K115R in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK1 (RIPK1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K115R

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabe-TetCMV-puro-mVenus-PA-Rac1-T17N

    Plasmid

    #22018

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mVenus-PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Rac1 starts at I4 and contains mutation T17N.

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pEF5/FRT/DEST-3xHA/mGli2 Pc-gA

    Plasmid

    #51255

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248 mutated to Ala

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pDest27-mBIN1+17

    Plasmid

    #61096

    Purpose

    Express N-terminal GST tagged-mouse BIN1+17 in mammalian cells

    Depositor

    Robin Shaw

    Article

    Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

    Insert

    mouse BIN1 with exon 17, excluding exon 7, 11, 13-16
    

    Tags

    GST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pLJM1 Flag GFP 4E-BP1 Rheb15

    Plasmid

    #112761

    Purpose

    express GFP-4EBP1-Rheb15

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-GFP-4EBP1-Rheb15 (EIF4EBP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag eGFP and Rheb15

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTH726-CEN-RLuc/minCFLuc

    Plasmid

    #38210

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3 (=GPD)

    Available Since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAFNF-PSDd1.2-GFP

    Plasmid

    #125581

    Purpose

    Flp-dependent expression of PSD d1.2-GFP (a deletion mutant of PSD-95 fused to GFP) in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    PSDd1.2-GFP (Dlg4 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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