We narrowed to 8,771 results for: ef1

1. LV_EF1a_ERalpha(Y537S)-P2A-Hygro_Barcode

   Plasmid

   #170225

   Purpose

   Barcoded lentiviral vector to express ERalpha (Y537S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   ER (ESR1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Y537S

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti-EF1a-Ly6G-miR30

   Plasmid

   #163358

   Purpose

   Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker.

   Depositor

   Louis Ates, Teunis Geijtenbeek

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Insert

   Ly-6G (Ly6g Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFIN-EF1a-CHER-WPRE

   Plasmid

   #44353

   Depositor

   Susan Semple-Rowland

   Article

   Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

   Insert

   EF1a-CHER
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   human elongation factor -1a (pos 2526-3969)

   Available since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEF1-puro/hpb1(short)

   Plasmid

   #59973

   Purpose

   integrin b1 short headpiece expression (beta-I, hybrid, PSI domains) expression

   Depositor

   Timothy Springer

   Insert

   human integrin b1 (short) (ITGB1 Human)
   

   Use

   Tags

   Base coiled-coil and His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a promoter

   Available since

   Sept. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

5. PB-CAG-Haunt-EF1a-DsRed

   Plasmid

   #92162

   Purpose

   Haunt in trans overexpression plasmid

   Depositor

   Xiaohua Shen

   Article

   Yin et al Cell Stem Cell. 2015 May 7;16(5):504-16.

   Insert

   Haunt (Halr1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPD1026 EF1alpha TetON3G in TU2

   Plasmid

   #139258

   Purpose

   Expresses TetON3G under hEF1alpha in TUPV2

   Depositor

   Joshua Leonard

   Insert

   TetON3G
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hEF1alpha

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAV-EF1α1.1-SomArchon-GFP

   Plasmid

   #153531

   Purpose

   AAV-mediated expression of SomArchon-GFP under the EF1α1.1 promoter.

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

   Insert

   SomArchon-GFP
   

   Use

   AAV

   Tags

   ER2, GFP, and KGC

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α1.1

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LLP251 pEF1a-UNC5C-4-SunTag

   Plasmid

   #100940

   Purpose

   TALE vector with UNC5C-4 insert fused to SunTagx10, driven by EF1a, with 3xHA tag, no PURO gene

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   TALE target sequence for UNC5C, SunTag array (10xGCN4)
   

   Use

   TALEN

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. miniTol2 x EF1a_EKAREN4(TA) + PGK-puro

   Plasmid

   #167826

   Purpose

   Control sensor for EKAREN4

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4(TA)
   

   Use

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W, T420A

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. miniTol2 x EF1a_EKAREN4 + PGK-blast

    Plasmid

    #167825

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCF820_U6-sgRNA-EF1a-mCherry2_lenti

    Plasmid

    #138316

    Purpose

    Lenti expression of mCherry2 with U6 promoter for SpyCas9 sgRNA targeting GFP

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    SpyCas9 GFP sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLENTI EF1alpha FLAG/HA-LCOR

    Plasmid

    #114444

    Purpose

    Mammalian lentiviral expression vector containing N terminal FLAG/HA tagged human LCOR coding sequence

    Depositor

    Adrian Bracken

    Article

    Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

    Insert

    LCOR (LCOR Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDSM-ab-Psptef1-NAT-Ttip1

    Plasmid

    #127747

    Purpose

    Yeast pathway position 2. NatMX transcription unit with the S. paradoxus TEF1 promoter and TIP1 terminator. Nourseothricin resistance.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    nourseothricin N-acetyltransferase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Psptef1

    Available since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDF1-S400F_RASA2-EF1-Puro

    Plasmid

    #114098

    Purpose

    Express mutant RASA2 in mamalian cells

    Depositor

    Yardena Samuels

    Article

    Arafeh et al Nat Genet. 2015 Dec;47(12):1408-10. doi:

    Insert

    RASA2 (RASA2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S400F

    Promoter

    CMV

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMSCV40-EF1a-hBCL10(1-185)-SO

    Plasmid

    #75258

    Purpose

    retroviral expression vector for expression of StrepOne-Tagged human BCL10 (AS 1-185)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    Bcl10 (BCL10 Human)
    

    Use

    Retroviral

    Tags

    StrepOne Tag

    Expression

    Mammalian

    Mutation

    AS 1-185 (w/o C-terminus)

    Promoter

    pMSCV-LTRs, EF1a

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LLP245 pEF1a-TALE-DNMT3a-Wt

    Plasmid

    #100937

    Purpose

    Empty TALE vector driven by EF1a promoter, no Puro, contains DNMT3a catalytic domain

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    TALE insertion site, DNMT3a catalytic domain (DNMT3A Human, Synthetic)
    

    Use

    TALEN

    Tags

    3xHA and 3xTy1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LLP338 pEF1a-DACH1-1-SunTag

    Plasmid

    #100942

    Purpose

    TALE vector with DACH1-1 insert fused to SunTagx10, driven by EF1a, with 3xHA tag, no PURO gene

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    TALE target sequence for DACH1, SunTag array (10xGCN4)
    

    Use

    TALEN

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAG75 ARHGEF18 3'UTR mut

    Plasmid

    #12055

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    N1 3'UTR mut (ARHGEF18 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Promoter

    Available since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-U6-sgNT-2-EF1a-LibVec

    Plasmid

    #239593

    Purpose

    expresses non-targeting (NT) guide#2

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Insert

    NT (non-targeting guide)
    

    Use

    AAV; To deliver a non-targeting guide (serving as…

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV-U6-sgPlxnb2-KO-4-EF1a-LibVec

    Plasmid

    #239600

    Purpose

    expresses guide#4 to knock-out mouse Plxnb2, via CRISPR-Cas9

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Insert

    Plxnb2 (Plxnb2 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAV-U6-sgPlxnb2-KO-3-EF1a-LibVec

    Plasmid

    #239599

    Purpose

    expresses guide#3 to knock-out mouse Plxnb2, via CRISPR-Cas9

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Insert

    Plxnb2 (Plxnb2 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AAV-U6-sgPlxnb2-KO-2-EF1a-LibVec

    Plasmid

    #239598

    Purpose

    expresses guide#2 to knock-out mouse Plxnb2, via CRISPR-Cas9

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Insert

    Plxnb2 (Plxnb2 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AAV-U6-sgPlxnb2-KO-1-EF1a-LibVec

    Plasmid

    #239597

    Purpose

    expresses guide#1 to knock-out mouse Plxnb2, via CRISPR-Cas9

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Insert

    Plxnb2 (Plxnb2 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AAV-U6-sgRNA-EF1a-LibVec

    Plasmid

    #239591

    Purpose

    AAV vector for cloning and expression of sgRNAs - concomitant expression of GFP serves as transfection marker

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAV-U6-sgNT-1-EF1a-LibVec

    Plasmid

    #239592

    Purpose

    expresses non-targeting (NT) guide#1

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Insert

    NT (non-targeting guide)
    

    Use

    AAV; To deliver a non-targeting guide (serving as…

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKG2116_pGEEC510_pShip-EF1a-mRuby2-bGH

    Plasmid

    #239716

    Purpose

    Plasmid expressing mRuby2 with the EF1a promoter and bGH 3' UTR

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pKG2117_pGEEC511_pShip-EF1a-mRuby2-SV40

    Plasmid

    #239717

    Purpose

    Plasmid expressing mRuby2 with the EF1a promoter and SV40 3' UTR

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKG2118_pGEEC512_pShip-EF1a-mRuby2-WPRE

    Plasmid

    #239718

    Purpose

    Plasmid expressing mRuby2 with the EF1a promoter and WPRE 3' UTR

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHAGE2-Ef1a-SOX17WT-3xFLAG

    Plasmid

    #216198

    Purpose

    Overexpress wild-type human SOX17 in HEK 293T cells for use in whole cell extract EMSAs

    Depositor

    Ralf Jauch

    Article

    Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

    Insert

    SOX17 (SOX17 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Glu113Glu (silent mutation); Glu120Glu (silent mu…

    Promoter

    Available since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHAGE2-Ef1a-SOX17E57D-3xFLAG

    Plasmid

    #216199

    Purpose

    Overexpress human SOX17E57D in HEK 293T cells for use in whole cell extract EMSAs

    Depositor

    Ralf Jauch

    Article

    Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

    Insert

    SOX17 (SOX17 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Glu113Glu (silent mutation); Glu120Glu (silent mu…

    Promoter

    Available since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHAGE2-Ef1a-SOX17E57P-3xFLAG

    Plasmid

    #216200

    Purpose

    Overexpress human SOX17E57P in HEK 293T cells for use in whole cell extract EMSAs

    Depositor

    Ralf Jauch

    Article

    Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

    Insert

    SOX17 (SOX17 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Glu113Glu (silent mutation); Glu120Glu (silent mu…

    Promoter

    Available since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHAGE2-Ef1a-OCT4-3xFLAG

    Plasmid

    #216196

    Purpose

    Overexpress human OCT4 in HEK 293T cells for use in whole cell extract EMSAs

    Depositor

    Ralf Jauch

    Article

    Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

    Insert

    OCT4 (POU5F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHAGE2-Ef1a-SOX2-3xFLAG

    Plasmid

    #216197

    Purpose

    Overexpress human SOX2 in HEK 293T cells for use in whole cell extract EMSAs

    Depositor

    Ralf Jauch

    Article

    Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHR Ef1a_LaG16-synNotch_tTA-PGK_Puro

    Plasmid

    #223536

    Purpose

    Anti-GFP LaG16 nanobody-based synNotch; puro-selectable

    Depositor

    Jonathan Brunger

    Article

    Walton et al Osteoarthritis Cartilage. 2025 Feb;33(2)

    Insert

    LaG16_synNotch_tTA
    

    Use

    Lentiviral

    Tags

    c-myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. EF1a-EGFP-FMRP-bGH

    Plasmid

    #235265

    Purpose

    ComMAND EGFP-FMRP base gene

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    EGFP-Fmr1 (Fmr1 Mouse)
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. EF1a-TS.FF4x1-mRuby2-bGH

    Plasmid

    #235274

    Purpose

    3' microRNA target site expression for dual-vector iFFL circuit (closed-loop)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. EF1a-mGL(miRE.FF4)-bGH

    Plasmid

    #235264

    Purpose

    microRNA expression for dual-vector iFFL circuit

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mGreenLantern
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. EF1a-TS.FF6x1-mRuby2-bGH

    Plasmid

    #235275

    Purpose

    3' microRNA target site expression for dual-vector iFFL circuit (open-loop)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. EF1a-mRuby2-TS.FF4x1-bGH

    Plasmid

    #235272

    Purpose

    3' microRNA target site expression for dual-vector iFFL circuit (closed-loop)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. EF1a-mRuby2-TS.FF6x1-bGH

    Plasmid

    #235273

    Purpose

    3' microRNA target site expression for dual-vector iFFL circuit (open-loop)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge