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Showing: 1201 - 1250 of 3198 results

  1. pLV(gRNA)-CMV-eGFP-U6(sgCTG)

    Plasmid
    #216732
    Purpose
    Contains a eGFP gene along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Used with the HD iPSC-derived astrocytes
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    sgCTG lentiviral guide RNA with eGFP tag
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216732-plasmid-map-sequence-id-430316

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  2. pDual-eGFP

    Plasmid
    #63215
    Purpose
    Expression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV-EF1α hybrid (CEF)
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. TLCV2-DelGFP

    Plasmid
    #133302
    Purpose
    EGFP reporter was removed in the TLCV2 vector so it could be used for inducible gene CRISPR knockout in cell lines with an eGFP reporter.
    Depositor
    Jingshi Shen
    Article
    Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    133302-plasmid-map-sequence-id-271497

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  22. LVDP 2P.2.EGFP

    Plasmid
    #231905
    Purpose
    For the production of SARS-CoV-2 virus-like particles (VLPs) in the '2-plasmid' system and packaging EGFP mRNA into the VLPs
    Depositor
    Sriram Neelamegham
    Article
    Yang et al npj Viruses. 2025 Jan; 3(4). doi: 10.103
    Insert
    nucleocapsid protein-T2A-membrane protein-IRES-envelope protein (N, E, M SARS-CoV-2)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    R203M in N protein
    Promoter
    CMV, EF-1a
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    231905-plasmid-map-sequence-id-459042

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  28. pWPT-mEGFP

    Plasmid
    #190606
    Purpose
    Obtained by creating a deletion inside the mCherry coding sequence in pWPT-/GCCACC-mEGFP-IRES-mCherry (Addgene #49235)
    Depositor
    Alessandro Quattrone
    Article
    Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    a deletion was created in mCherry CDS impairing i…
    Promoter
    EIF1-short
    Available since
    Oct. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    190606-plasmid-map-sequence-id-375284

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  37. WT Smarca4-sfGFP

    Plasmid
    #107056
    Purpose
    Expression of wild-type Smarca4 (Brg1) with C-terminal super-folder GFP fusion.
    Depositor
    Jerry Crabtree, Courtney Hodges
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    Super-folder GFP (sfGFP)
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CAG
    Available since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107056-plasmid-map-sequence-id-321500

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  43. LV-Sox10MCS5-GFP

    Plasmid
    #115783
    Purpose
    This plasmid encodes for Sox10-MCS5-GFP reporter. Cell carrying this construct expresses green fluorescence under the control of SOX-MCS5 enhancer conjugated with cfos basal promoter.
    Depositor
    Fraser Sim
    Article
    Pol et al Exp Neurol. 2013 Sep;247:694-702. doi: 1
    Insert
    mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
    Use
    Lentiviral
    Tags
    cfos basal promoter conjugated MCS5 promoter fuse…
    Expression
    Mutation
    Promoter
    SOX10 Multiple Species Conserved enhancer element…
    Available since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    115783-plasmid-map-sequence-id-226907

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  47. pCSIIbleo-miRFP670nano(R57C/C86S)-P2A-EGFP

    Plasmid
    #197370
    Purpose
    The lentiviral vector for miRFP670nano(R57C/C86S) and EGFP
    Depositor
    Michiyuki Matsuda
    Insert
    miRFP670nano(R57C/C86S)
    Use
    Lentiviral
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    R57C/C86S mutation
    Promoter
    CAG
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    197370-plasmid-map-sequence-id-389688

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  48. pCSIIbleo-iRFP713(C15S/V256C)-P2A-EGFP

    Plasmid
    #198062
    Purpose
    The lentiviral vector for iRFP713(C15S/V256C) and EGFP
    Depositor
    Michiyuki Matsuda
    Insert
    iRFP713(C15S/V256C)
    Use
    Lentiviral
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    C15S/V256C mutation
    Promoter
    CAG
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198062-plasmid-map-sequence-id-389830

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Showing: 1201 - 1250 of 3198 results