Skip to main content
Addgene
Showing: 481 - 500 of 5682 results

  3. pBW014

    Plasmid
    #131817
    Purpose
    Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 55 and 56
    Depositor
    Jonathan Silberg
    Article
    Wu et al bioRxiv May 23, 2019
    Insert
    M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 55 and 56
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    131817-plasmid-map-sequence-id-259073

    Enlarge

  4. pBW010

    Plasmid
    #131812
    Purpose
    Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 35 and 36
    Depositor
    Jonathan Silberg
    Article
    Wu et al bioRxiv May 23, 2019
    Insert
    M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 35 and 36
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    131812-plasmid-map-sequence-id-259033

    Enlarge

  5. pBW008

    Plasmid
    #131809
    Purpose
    Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 30 and 31
    Depositor
    Jonathan Silberg
    Article
    Wu et al bioRxiv May 23, 2019
    Insert
    M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 30 and 31
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    131809-plasmid-map-sequence-id-259026

    Enlarge

  6. pBW006

    Plasmid
    #131807
    Purpose
    Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 25 and 26
    Depositor
    Jonathan Silberg
    Article
    Wu et al bioRxiv May 23, 2019
    Insert
    Mastigocladus laminosus ferredoxin mutant with the estrogen recepor-ligand binding domain fused between amino acids 25 and 26
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    131807-plasmid-map-sequence-id-258996

    Enlarge

  7. pVH001

    Plasmid
    #80394
    Purpose
    Contains aTc inducible Scaffold-mCherry-AAV and TetR genes
    Depositor
    Richard Murray
    Article
    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.
    Insert
    mCherry RFP
    Use
    Synthetic Biology
    Tags
    Fused by GS linkers to Sh3pep-LZX scaffold protei…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    80394-plasmid-map-sequence-id-155809

    Enlarge

  8. pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

    Plasmid
    #238007
    Purpose
    300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.
    Depositor
    Simon Powell, Agnel Sfeir
    Article
    Jalan et al Nat Commun. 2025 May 10;16(1):4349. doi:
    Insert
    AAVS1 (AAVS1, PPP1R12C Human)
    Use
    Rna donor to study rt-dsbr
    Tags
    Expression
    Mammalian
    Mutation
    3bp insertion signature (ATC) at Cas9 cut site in…
    Promoter
    CMV
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238007-plasmid-map-sequence-id-472649

    Enlarge

  9. pYHY3

    Plasmid
    #216109
    Purpose
    aTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.
    Depositor
    Shannon Sirk
    Article
    Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:
    Insert
    NanoLuc
    Use
    Synthetic Biology; E. coli-bacteroides shuttle ve…
    Tags
    3xFLAG tag and BT3630 signal peptide
    Expression
    Bacterial
    Mutation
    Promoter
    P1TDP-A21
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216109-plasmid-map-sequence-id-434372

    Enlarge

  15. pTPGI_dCas9_VP64

    Plasmid
    #49013
    Purpose
    encodes yeast-optimized dCas9_VP64 synthetic transcription factor
    Depositor
    Timothy Lu
    Article
    Farzadfard et al ACS Synth Biol. 2013 Sep 11.
    Insert
    dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Promoter
    pTPGI (galactose+aTc inducible)
    Available since
    Jan. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    49013-plasmid-map-sequence-id-231494

    Enlarge

  20. pMD19T-slr0230-PL22-sgRNANT1-KmR

    Plasmid
    #73224
    Purpose
    Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli
    Depositor
    Paul Hudson
    Article
    Yao et al ACS Synth Biol. 2015 Dec 28.
    Insert
    PL22-sgRNA NT1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PL22
    Available since
    April 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73224-plasmid-map-sequence-id-135053

    Enlarge

Showing: 481 - 500 of 5682 results