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pcDNA3.1 LIC 6A HIS-TEV-DPP8

Plasmid

#166793

Purpose

Mammalian cell expression vector for Flag-tagged DPP8

Depositor

Hao Wu

Publication

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP8 (DPP8 Human)

Tags

HIS-TEV

Expression

Mammalian

Promoter

CMV

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pRSETB/mStayGold(c4)

Plasmid

#212018

Purpose

For N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.

Depositor

Atsushi Miyawaki

Publication

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Type

Empty backbone

Tags

6xHis, T7 tag, Xpress tag, mStayGold(c4)

Expression

Bacterial

Promoter

T7

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-REL

Plasmid

#27253

Depositor

Thomas Gilmore

Publication

Leeman et al Oncogene. 2008 Dec 4. 27(53):6770-81.

Insert

REL (REL Human)

Tags

FLAG

Expression

Mammalian

Available since

Feb. 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pACYC-Taq

Plasmid

#102793

Purpose

Expresses Taq DNA polymerase under WT T7 promoter

Depositor

Andrew Ellington

Publication

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Taq DNA polymerase

Expression

Bacterial

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S LL100EE

Plasmid

#166798

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. LL100EE mutant is loss of CARD8 and NLRP1 binding.

Depositor

Hao Wu

Publication

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

FLAG-TEV

Expression

Mammalian

Mutation

LL100EE

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S S730A

Plasmid

#166801

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. The S730A mutant is catalytically dead.

Depositor

Hao Wu

Publication

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

FLAG-TEV

Expression

Mammalian

Mutation

S730A

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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MLV Gag-RFP

Plasmid

#1814

Depositor

Walther Mothes

Publication

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

Gag (gag MuLV)

Tags

RFP

Expression

Mammalian

Available since

May 19, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pOPINF

Plasmid

#26042

Depositor

Ray Owens

Publication

Berrow et al Nucleic Acids Res. 2007. 35(6):e45.

Insert

His3C-lacZ

Tags

MAHHHHHHSSGLEVL

Expression

Bacterial, Insect, and Mamm…

Available since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pKIKOarsBKm

Plasmid

#46766

Purpose

E.coli genomic integration at arsB

Depositor

Lars Nielsen, Claudia Vickers

Publication

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Available since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Nanog-2A-mCherry

Plasmid

#59995

Purpose

Inserts mCherry immediately downstream of the Nanog coding region

Depositor

Rudolf Jaenisch

Publication

Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

Insert

Nanog (Nanog Mouse)

Use

Mouse Targeting

Tags

2A-mCherry

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pDR-GW AT1.03

Plasmid

#28003

Depositor

Wolf Frommer

Publication

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

AT1.03

Tags

mVenus and mseCFP

Expression

Yeast

Available since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pDiGc

Plasmid

#59322

Purpose

Inducible expression of dsRed and constitutive expression of GFP in bacteria

Depositor

Sophie Helaine, David Holden

Publication

Helaine et al Proc Natl Acad Sci U S A. 2010 Feb 23;10

Inserts

EGFP
dsRed

Expression

Bacterial

Promoter

Pbad and rpsM

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pcDNA3/mStayGold(c4)=UtrCH

Plasmid

#212020

Purpose

For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Publication

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

UtrCH

Tags

mStayGold(c4)

Expression

Mammalian

Promoter

CMV

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLCA.66/2272

Plasmid

#22733

Depositor

Mark Magnuson

Publication

Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

Insert

Lox66-pU(delta)TK-EM7Neo-Lox2272

Use

Cre/Lox; Bac recombineering

Mutation

This vector was designed for assembling gene targ…

Available since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pcDNA3.1 Dendra2

Plasmid

#103967

Purpose

Mammalian expression of fluorescent protein Dendra2 used for transfection efficiency control

Depositor

Qing Deng

Publication

Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

Insert

Dendra2

Expression

Mammalian

Available since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pF150 pSOD1G93AAcGFP1

Plasmid

#26411

Purpose

contains G94A mutation in pSOD1

Depositor

Elizabeth Fisher

Publication

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

G94A

Available since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pET42b-BE3

Plasmid

#87437

Purpose

Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Publication

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

BE3 (Apobec1 Rat, Bacillus phage PBS2, Human)

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSFV-Helper1

Plasmid

#92073

Purpose

This is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.

Depositor

Henrik Garoff

Publication

Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

Insert

cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.

Use

Helper plasmid, for run-off transcription and rna…

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEGFPC1-6XHis-FLKSRP

Plasmid

#23001

Depositor

Douglas Black

Publication

Hall et al Mol Biol Cell. 2004 Feb . 15(2):774-86.

Insert

KSRP (KHSRP Human)

Tags

EGFP and His

Expression

Mammalian

Mutation

valine inserted at aa#2

Available since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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YFP NLS Beta-Actin

Plasmid

#60613

Purpose

Encodes YFP and NLS tagged beta-actin

Depositor

Primal de Lanerolle

Publication

Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

Beta-actin (ACTB Human)

Tags

EYFP and NLS

Expression

Mammalian

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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