Addgene

We narrowed to 21,407 results for: ESC;

Showing: 7471 - 7500 of 21407 results

Results per page:

Sort results by:

pHEA

Plasmid

#168297

Purpose

Bacterial surface display of polypeptides (nanobodies) in E. coli

Depositor

Luis Ángel Fernández

Article

Marin et al J Bacteriol. 2010 Nov;192(21):5588-602.

Insert

Enterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)

Use

Tags

6xHis, E-tag, and PelB signal peptide

Expression

Bacterial

Mutation

Promoter

LacI-Plac

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-ECFPEYFP

Plasmid

#24520

Depositor

Liusheng He, Jehonathan Pinthus

Article

He et al Cytometry A. 2003 Oct . 55(2):71-85.

Insert

ECFP, EYFP

Use

Tags

ECFP and EYFP

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
pRN12

Plasmid

#84456

Purpose

shuttle plasmid for sarA P1-mAmetrine expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

mAmetrine

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE161

Plasmid

#79453

Purpose

recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Tags

Expression

Plant

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pKIKOrbsARKm

Plasmid

#46768

Purpose

E.coli genomic integration rbsAR locus

Depositor

Claudia Vickers, Lars Nielsen

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
ecAT3.10

Plasmid

#107215

Purpose

Mammalian expression plasmid to target the ecAT3.10 extracellular ATP sensor to the cell surface.

Depositor

Mathew Tantama

Article

Conley et al PLoS One. 2017 Nov 9;12(11):e0187481. do

Insert

ecAT3.10

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTarget12f1

Plasmid

#171183

Purpose

Constitutive expression of sgRNA without donor DNA

Depositor

Kohsuke Honda

Article

Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

Insert

sgRNA scaffold in the CRISPR/Cas12f1 system

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119(SpeI) promoter

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMUT2-pLac-mCherry

Plasmid

#192859

Purpose

pMUT2 based vector for sensing lactic acid in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

lldR operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-HisD-CreiLOV

Plasmid

#192817

Purpose

Expresses CreiLOV in bacteria

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

CreiLOV

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Ateam1.03-nD/nA/pENTR1A

Plasmid

#51959

Purpose

Gateway entry vector containing ATeam1.03-nD/nA, a FRET-based ATP indicator

Depositor

Takeharu Nagai

Article

Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

Insert

ATeam1.03-nD/nA

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
mScarlet-I-mTurquoise2

Plasmid

#98839

Purpose

Produces a fusion between mTurquoise2 and mScarletI. It can be used as a positive control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mScarletI-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yomCherry-CaURA3

Plasmid

#44876

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mCherry with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

Plasmid

#45565

Purpose

Expresses I-Sce I nuclease in mammalian cells and BFP as a tracker

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Inserts

I-Sce I Nuclease
BFP

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU45

Plasmid

#167667

Purpose

ENTRY vector for gRNA5 cloning under pAtU6 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG25-pBAD-tetO-deGFPssrA

Plasmid

#102452

Purpose

Expresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

deGFP

Use

Tags

ssrA

Expression

Bacterial

Mutation

Promoter

pBAD-tetO

Available since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU46

Plasmid

#167668

Purpose

ENTRY vector for gRNA6 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mTurquoise2-Rab7(WT)

Plasmid

#112959

Purpose

In vivo visualization of Rab7 (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

Rab7

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Ii-Str_SBP-EGFP-GPI

Plasmid

#65296

Purpose

synchronize trafficking of EGFP-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Use

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pNOC-ARS-stacked-NeoR-mNeonGreen

Plasmid

#101249

Purpose

Construct for fusion expression of mNeonGreen reporter and G418 resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Tags

Expression

Mutation

Promoter

Ribi

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT_TO_FlagHA_USP10_C424A

Plasmid

#127103

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

USP10 (USP10 Human)

Use

Tags

Expression

Mammalian

Mutation

C424A

Promoter

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Ateam1.03-nD/nA/pRSETB

Plasmid

#51960

Purpose

bacterial expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

Depositor

Takeharu Nagai

Article

Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

Insert

ATeam1.03-nD/nA

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
myr-palm_mCherry2-mEGFP

Plasmid

#127816

Purpose

Hetero-dimer mCherry2-mEGFP expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

myristoylated-palmitoylated-mCherry2-mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbA5c-MevT-MBI (JBEI-3085)

Plasmid

#87950

Purpose

the overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP).

Depositor

Taek Soon Lee

Article

Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

Insert

MevT-MBI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
EB3-mScarlet-I

Plasmid

#98826

Purpose

In vivo visualization of microtuble plus ends (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

EB3

Use

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAJM.683

Plasmid

#108531

Purpose

BetIAM + PBetI-YFP Reporter. Cho sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

BetIAM + PBetI-YFP

Use

Tags

Expression

Bacterial

Mutation

BetI: S115I

Promoter

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG461-FRB-redNFAST

Plasmid

#160362

Purpose

Expresses FRB-redNFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

FRB-redNFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yomKate2-SpHis5

Plasmid

#44852

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKate2 with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKate2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
mTagBFP2-SiT-N-15

Plasmid

#55325

Purpose

Localization: Golgi TGN, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

SiT (ST6GAL1 Human)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

aa 1-45 of SiT

Promoter

CMV

Available since

Oct. 24, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pSC101-GFPmut2-mScarlet-I TAG

Plasmid

#208188

Purpose

nonsense mistranslation, TAG stop

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Tags

Expression

Bacterial

Mutation

Insertion 6TAG*(stop) 7AGT(Ser)

Promoter

Ptet+dnaK P1

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSC101-GFPmut2-mScarlet-I TGA

Plasmid

#208189

Purpose

nonsense mistranslation, TGA stop

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Tags

Expression

Bacterial

Mutation

Insertion 6TGA*(stop) 7AGT(Ser)

Promoter

Ptet+dnaK P1

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge