We narrowed to 16,478 results for: por
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pSC-mCh-P2A-ST-EGFP-CAAX
Plasmid#207636PurposeA plasmid encoding photocaged SpyCatcher (pSC) fused to mCherry and a SpyTag-EGFP localized to the cell membrane via CAAX tagDepositorInsertphotocaged SpyCatcher-mCh-P2A-SpyTag-EGFP-CAAX
UseTagsExpressionMammalianMutationAmber stop codon at SpyCatcher’s critical lysinePromoterCMVAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
LUC01 (L/Sec/P)
Plasmid#112144PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Photinus pyralis, Rat)
UseTagsExpressionMammalianMutationCysteine 258 mutated to selenocysteine (U)PromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
nuc-GafD-mTurboID-HA
Plasmid#184641PurposeExpresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome taggingDepositorInsertnuc-GlycoID-HA
UseTags3xHA tag and Nuclear localization sequenceExpressionMammalianMutationGafD -> expressed 22-178 ; BirA* -> express…PromoterCMVAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GalT-mCherry
Plasmid#214271PurposeMammalian expression of GalT-mCherry (Golgi marker)DepositorInsertGalT(1-82)-mCherry (B4GALT1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNKX2-5-T2A-GFP-PGK-Puro
Plasmid#203607PurposeDonor plasmid to create 3' knockin of NKX2-5 with T2A-GFPDepositorInsertNKX2-5-T2A-GFP-PGK-Puro-NKX2-5 (NKX2-5 Human)
UseHuman targetingTagsExpressionMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWZ854
Plasmid#208116PurposemSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-NeoDepositorInsertmNeonGreen-HPRT1-Neo
UseTagsT2AExpressionBacterial and MammalianMutationPromoterEF1alphaAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWZ852
Plasmid#208114PurposemSwAP-In marker cassette 1 with mScarlet-HygTKDepositorInsertmScarlet-HygTK
UseTagsT2AExpressionBacterial and MammalianMutationPromoterEF1alphaAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHD-SPARC2-S-LexA::p65
Plasmid#133564PurposeSwap out effector and terminator to generate SPARC2-S CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locusDepositorInsertLexA::p65
UseCRISPRTagsExpressionInsectMutationPromoter20X UASAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV2_hSyn_NES-Caprola_off-mEGFP_WRPE-SV40
Plasmid#194692PurposehSyn1 driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through AAV transductionDepositorInsertCaprola_off-mEGFP
UseAAVTagsmEGFPExpressionMutationPromoterhSyn1Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
YCplac33-NAT_MCART1
Plasmid#140591PurposeExpress MCART1 in S. cerevisiaeDepositorInsertSLC25A51 with 5' and 3' UTR of scNDT1 (SLC25A51 Human)
UseTagsExpressionYeastMutationcodon-optimized for expression in S. cerevisiaePromoterNDT1 promoter and 3'UTRAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-mCherry-FRB*
Plasmid#207145PurposeLysosome-targeted rapalog dimerizerDepositorInsertLAMP1 (LAMP1 Human)
UseTagsFRB* (T2098L mutant of FKBP-rapamycin binding dom…ExpressionMammalianMutationSilent mutationPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Y1175F-VEGFR2-mCherry
Plasmid#139608PurposeExpresses a mutant (Y1175F) VEGFR2-mCherry fusion protein in mammalian cellsDepositorInsertKDR (KDR Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cox7c-pHLuorin-sems
Plasmid#107766Purposelabeling of mitochondrial cytochrome c oxidase, pH measurementsDepositorInsertSubunit Cox7c fused to pHluorin (COX7C Human)
UseTagspHluorinExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-Macro-Linker-eGFP
Plasmid#176067PurposeEGFP fused to the C-terminus of a Macrodomain & a hygromycin resistance cassetteDepositorInsertMacro
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHD-SPARC2-D-LexA::p65
Plasmid#133562PurposeSwap out effector and terminator to generate SPARC2-D CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locusDepositorInsertLexA::p65
UseCRISPRTagsExpressionInsectMutationPromoter20X UASAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV[Exp]-Neo-EF1A>Tet3G
Plasmid#184379PurposeThe Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)DepositorInsertTet3G
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-WWE-Linker-eGFP
Plasmid#176072PurposeEGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassetteDepositorInsertWWE
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-hCD24-T2A-GFP
Plasmid#205456Purposelentiviral plasmid for expression of human CD24DepositorInserthCD24 (CD24 Human)
UseTagsExpressionMammalianMutationPromoterUBCAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-Cbir-Ago2_Myc-Nbir-Dicer
Plasmid#90013PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-DicerDepositorInsertsCBirA*-Ago2
NBirA*-Dicer
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
LUC05 (L/Cys/P)
Plasmid#112146PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with wild type Cysteine 258 and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Photinus pyralis, Rat)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-PBZC-Linker-eGFP
Plasmid#176070PurposeEGFP fused to the C-terminus of a PBZ domain & a hygromycin resistance cassetteDepositorInsertPBZ-C
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-PBM-Linker-eGFP
Plasmid#176069PurposeEGFP fused to the C-terminus of a PBM domain & a hygromycin resistance cassetteDepositorInsertPBM
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-RON-V5/HIS
Plasmid#201983Purposeexpression of human RON receptor tyrosine kinase in mammalian cellsDepositorInsertRON receptor tyrosine kinase (MST1R Human)
UseTagsV5, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB80-LAMP1-mCherry-iLID
Plasmid#174625PurposeOptogenetic coupling to lysosomes (LAMP1) via iLIDDepositorInsertLAMP1-mCherry-iLID (LAMP1 Human, Synthetic)
UseTagsmCherry-iLIDExpressionMammalianMutationmCherry: Met1DelPromoterChicken beta-actinAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MCU-D261A
Plasmid#31729DepositorInsertMCU (MCU Human)
UsePdonr gatewayTagsNoneExpressionMutationChanged D261 to AlaninePromoterAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-WDR5
Plasmid#200880PurposeMammalian expression of human WDR5 with N-terminal HaloTag fusionDepositorInsertHaloTag-WDR5 (WDR5 Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1alpha-soCoChR-tdTomato
Plasmid#107714PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoterDepositorInsertsoCoChR-tdTomato
UseAAVTagsKA2 and tdTomatoExpressionMammalianMutationPromoterEF1alphaAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC-cUnaG-P2A-nUnaG-ST-P2A-mCh
Plasmid#207634PurposeA plasmid encoding photocaged SpyCatcher (pSC)-cUnaG, nUnaG-SpyTag (ST), and mCherry separated by P2A cleaving sequences for mammalian expression.DepositorInsertphotocaged SpyCatcher-cUnaG-P2A-nUnaG-SpyTag-P2A-mCh
UseTagsExpressionMammalianMutationAmber stop codon at SC's critical lysinePromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin-FLEX-soCoChR-GFP
Plasmid#107712PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Cre-dependent virusDepositorHas ServiceAAV9InsertsoCoChR-GFP
UseAAVTagsEGFP and KA2ExpressionMammalianMutationPromoterhSynapsinAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only
