We narrowed to 6,934 results for: CAD

1. pFRLuc-CoV2-delStem1

   Plasmid

   #177616

   Purpose

   Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. 3-nucleotide deletion in Stem 1 (negative control).

   Depositor

   Junjie Guo

   Article

   Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

   Insert

   SARS-CoV-2 FSE
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   3-nucleotide (UAC) deletion in Stem 1

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Chr7_Centromere-Targeting_gRNA

   Plasmid

   #195129

   Purpose

   dual gRNA vector targeting centromere-proximal locations on Chromosome 7p and 7q in a third generation Cas9 backbone with GFP

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   Chr7 gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cav2.2 BBS pcDNA3

   Plasmid

   #206057

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II

   Depositor

   Annette Dolphin

   Article

   Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTSlb-N748D

   Plasmid

   #60732

   Purpose

   Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations N748D.

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Inserts

   Residues 1-179 of split T7 RNAP
   Residues 180-880 of split T7 RNAP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Residues 1-180 of split T7 RNAP and Residues 180-…

   Promoter

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXs-hLIN28A

   Plasmid

   #47902

   Purpose

   Expresses human LIN28A in mammalian cells using retrovirus system.

   Depositor

   Shinya Yamanaka

   Article

   Tanabe et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

   Insert

   human LIN28A (LIN28A Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Oct. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBSFI-myr-akt-T308A/S473A

   Plasmid

   #49191

   Purpose

   cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 and S to A at position 473 in cloning vector

   Depositor

   Peter Vogt

   Article

   Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Adaptor plasmid

   Tags

   HA tag and myristolation signal

   Expression

   Mutation

   T308A; S473A

   Promoter

   T7

   Available since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAPM-miR30-MAVS-ts1

   Plasmid

   #174274

   Purpose

   MAVS knockdown

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   MAVS shRNA (MAVS Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. 904 pLNCX HA Akt1 K179M

   Plasmid

   #9007

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   Akt1 K179M (AKT1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K179M. Kinase dead.

   Promoter

   Available since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

9. canxTM-mEmerald (cytosolic)

   Plasmid

   #186962

   Purpose

   Expresses mEmerald connected with the C-terminus of Calnexin transmembrane domain.

   Depositor

   Ke Xu

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   CANX (CANX Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Only express calnexin (482-end) and add ER signal…

   Promoter

   CMV Promoter

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR221-FGFR4

    Plasmid

    #60531

    Purpose

    Entry vector for human FGFR4

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    FGFR4 (FGFR4 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    c2134a P712T

    Promoter

    NA

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pMKO shRNA Bim

    Plasmid

    #17235

    Depositor

    Joan Brugge

    Article

    Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

    Insert

    Bim shRNA (BCL2L11 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pFastBac1-LRRK2 WD40

    Plasmid

    #139211

    Purpose

    Human LRRK2 WD40 domain (residues 2142–2527) was expressed in Sf9 insect cells via baculovirus infection and purified to homogeneity.

    Depositor

    Hao Wu

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2019 Jan 29;11

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    N-His and MBP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. aav-tnt-actn2-gfp

    Plasmid

    #165034

    Purpose

    AAV-based delivery of ACTN2-GFP into cardiomyocytes in vivo

    Depositor

    William Pu

    Article

    Guo et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

    Insert

    ACTN2 (Actn2 Mouse)
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mutation

    Promoter

    cTNT

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDL691

    Plasmid

    #231166

    Purpose

    T-DNA encoding TRV2 with ipt and mobile gRNAs targeting SlUGT75C1 and SlHAIRLESS

    Depositor

    Daniel Voytas

    Article

    Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

    Insert

    ipt and mobile gRNAs targeting SlUGT75C1 and SlHAIRLESS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    PEBV sub-genomic

    Available since

    Jan. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28_6xHis-PARP1-CAT-L713F

    Plasmid

    #173944

    Purpose

    Bacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutation

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-CAT-L713F (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L713F, V762A

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLL7.0: mTiam1(64-437)-tgRFPt-SSPB R73Q

    Plasmid

    #60418

    Purpose

    Mammalian Expression of Tiam DH/PH-tgRFPt-Micro

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    mTiam1-tgRFPt-SSPB R73Q
    

    Use

    Cre/Lox and Lentiviral

    Tags

    tgRFPt

    Expression

    Mammalian

    Mutation

    mTiam 64-437 // SSPB R73Q, Y11K and A15E

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEGFP-K685R-STAT3

    Plasmid

    #71449

    Purpose

    Retroviral expression of K685R-STAT3. Please note that this plasmid contains K685R-STAT3 tagged with FLAG, not GFP.

    Depositor

    George Stark

    Article

    Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;11

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mutation

    K685R

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pCEP4-myc-HLA-B57

    Plasmid

    #135516

    Purpose

    Mammalian expression of myc-tagged HLA-B*57:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-B*57:1 (HLA-B Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDropIn-T2A-split-intein-Gal4[1-20]-N-int-BlastR

    Plasmid

    #199197

    Purpose

    Insert to create split-intein Gal4[N-int] knock-in vectors, with blasticidin resistance, via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[1-20]-N-int-BlastR
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDropIn-T2A-split-intein-Gal4[21-881]-C-int-G418R

    Plasmid

    #199198

    Purpose

    Insert to create split-intein Gal4[21-881]-C-int knock-in vectors, with G418 resistance, via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[21-881]-C-int-G418R
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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