We narrowed to 44,705 results for: cha;
-
Plasmid#177826PurposeSite-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).DepositorInsertGFP_C289T
ExpressionMammalianMutationC289TPromoterCMVAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
MBP-TSF-TEVcs-ULK1K46I
Plasmid#171417PurposeFor the purifcation of human ULK1 kinase dead complexDepositorAvailable SinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPOP-V5 (BTB)
Plasmid#169721PurposeMammalian Expression of mouse SPOP-BTB (L186D, L190D, L193D, I217K)DepositorInsertSPOP (Spop Mouse)
TagsV5ExpressionMammalianMutationL186D, L190D, L193D, I217KPromoterCMVAvailable SinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCry2PHR-mCh-RhoA
Plasmid#42958PurposeExpresses optogenetically controlled RhoADepositorAvailable SinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pExodus CMV.Trex2
Plasmid#40210PurposeMammalian expression of codon-optimized Trex2.DepositorInsertThree prime repair exonuclease 2 (Trex2 Mouse)
ExpressionBacterial and MammalianPromoterCMV and T7Available SinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-20b-M-Ntx-S1
Plasmid#58716PurposeExpresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1SDepositorInsertNotexin
TagsMethionineExpressionBacterialMutationChanged Asparagine 1 to SerinePromoterT7Available SinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-IRAlus-Lin28
Plasmid#92347PurposePlasmid for expression of GFP gene with a Lin28 gene derived IR Alu element containing 3'UTR.DepositorInsertLin28 gene derived IR Alu element containing 3'UTR (LIN28A Human)
ExpressionMammalianPromoterCMVAvailable SinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
SNX10-PX (1-201)
Plasmid#119091PurposeBacterial expression of human phox homology (PX) domain, SNX10-PX (1-201)DepositorAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pclbw-eGFPomp25
Plasmid#58424PurposeMammalian expression of GFP targeted to the mitochondrial outer membraneDepositorInserteGFP-omp25 (Synj2bp Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationcontains aa's 109-145 of mouse omp25PromoterCMV/b-actinAvailable SinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Mfn2(K109A)-16xmyc
Plasmid#26051DepositorAvailable SinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-pcDronpa2-A69T
Plasmid#98578Purposephotoconvertible fluorescent protein pcDronpa2-A69T in pRSetB plasmid, photoconvertible by primed conversion mechanism, bacterial overexpressionDepositorInsertpcDronpa2-A69T
Tags6xHisExpressionBacterialPromoterT7Available SinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMax_BFP_A191G
Plasmid#177823PurposeSite-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).DepositorInserttagBFP_A191G
ExpressionMammalianMutationA191GPromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TEV-TBP6.9F34MF37MF77M
Plasmid#168268PurposeExpresses human TBP6.9 with the F34M/F37M/F77M triple mutantDepositorInsertTBP6.9
Tags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F34M, F37M and F77MPromoterT7 promotorAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Teto Ngn2
Plasmid#62223PurposeInducible expression of Ngn2DepositorAvailable SinceMarch 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
CFAST-Halotag-6xHp
Plasmid#214416PurposeLipid droplet localization of CFAST fused to Halotag-6xHpDepositorInsertdeletion
TagsCFAST-HaloExpressionMammalianPromoterCMVAvailable SinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
N-BLa 1.2
Plasmid#88999PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.DepositorInsertN-BLa 1.2 fusion protein
TagsFlagExpressionBacterialPromoteraraBADAvailable SinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
C-BLa 1.2
Plasmid#89000PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the C-terminal fragment of the split β-lactamase.DepositorInsertC-BLa 1.2 fusion protein
TagsFlagExpressionBacterialPromoteraraBADAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
FAB(PX-LD)
Plasmid#214426PurposeDetection of peroxisome-lipid droplet contact siteDepositorInsertFKBP-V5
TagsCFAST-Halo and FKBP-V5-RspANExpressionMammalianPromoterCMVAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-VdC9BV
Plasmid#62195PurposeExpresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous GenesDepositorInsertVP64dCas9BFPVP64
UseLentiviralTagsTwo VP64s tagged to dCas9 fused to BFPExpressionMammalianMutationD10A H840A (catalytically inactive) Cas9 (dCas9)Available SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRR-ERbeta-5Z
Plasmid#23062DepositorInsertERbeta (ESR2 Human)
ExpressionYeastAvailable SinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Alu-Lin28
Plasmid#92346PurposePlasmid for expression of GFP gene with a Lin28 gene derived Alu element containing 3'UTR.DepositorInsertLin28 gene derived Alu element containing 3'UTR (LIN28A Human)
ExpressionMammalianPromoterCMVAvailable SinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSG5-HA-ERR3 S45A
Plasmid#37849DepositorInsertEstrogen-related receptor gamma (Esrrg Mouse)
TagsHAExpressionMammalianMutationchanged Serine 45 to Alanine (AGC to GCC)PromoterSV40Available SinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
AAV r3.45
Plasmid#233684PurposeAn evolved Adeno-associated viral variant, enhances gene delivery and gene targeting in neural stem cellsDepositorInsertModified AAV2 Rep, Cap
UseAAVMutationinsertion of LATQVGQKTA (where LA and A are linke…Available SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaRIgG-2
Plasmid#228996PurposeFor bacterial expression of anti-rabbit IgG nanobody LaRIgG-2, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaRIgG-2
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaGIgG-12
Plasmid#228999PurposeFor bacterial expression of anti-mouse IgG nanobody LaMIgG-12, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaGIgG-12
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
mPA-GFP-Actin-7
Plasmid#57120PurposeLocalization: Actin, Excitation: 400 / 504, Emission: 515 / 517DepositorAvailable SinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pECE M2-SNX17 WT
Plasmid#69811PurposeExpresses M2-SNX17 in mammalian cellsDepositorAvailable SinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5 CASTOR1-Flag Cterm
Plasmid#84488Purposeoverexpression of CASTOR1 with Flag tagDepositorAvailable SinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-mMaple3-V157I
Plasmid#98577Purposephotoconvertible fluorescent protein mMaple3-V157I in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coliDepositorInsertmMaple3-V157I
Tags6xHisExpressionBacterialPromoterT7Available SinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
FKBP-CD28TMD-NTEVp(H75E)
Plasmid#137829PurposeMESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75E mutant TEV proteaseDepositorInsertFKBP-CD28TMD-NTEVp(H75E)
Tags3x FLAGExpressionMammalianMutationMutated Histidine 75 to Glutamic Acid in NTEVp fr…PromoterCMVAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCyPet-Rac1(Q61L)
Plasmid#22783DepositorAvailable SinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLv-CMV-MyoD
Plasmid#26808DepositorInsertMyoD (Myod1 Mouse)
UseLentiviralAvailable SinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSC-PreE2
Plasmid#52264PurposeBacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with PreScission protease cleavage site linkerDepositorAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
pPPEDs
Plasmid#162469Purposeprotoplast test vector of pPPEDDepositorInsertsCas9H840A
attR gateway destination cassette
UsePrime editing destination vector for transient ex…TagsMLV reverse transcriptaseExpressionPlantMutationChange histidine 840 to AlanineAvailable SinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
SNX29-PX (269-391)
Plasmid#119108PurposeBacterial expression of human phox homology (PX) domain, SNX29-PX (269-391)DepositorInsertSNX29-PX (269-391)
TagsGSTExpressionBacterialPromotertacAvailable SinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEC3111 (pSpy1C-Plac(3)_ffluc)
Plasmid#218778Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(3)Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-STChRger2-TS-EYFP
Plasmid#129393PurposeSoma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.DepositorInsertsoma targeted ChRger2
UseAAVTagsKv2.1-TS-EYFPExpressionMammalianPromoterCaMKIIaAvailable SinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ITR-PytR-mCherry
Plasmid#204868PurposeExpresses pyrrolysyl tRNA and a wild-type mCherry reporter; can be packaged into AAVDepositorInsertM. mazei pyrrolysyl tRNA
UseAAVExpressionMammalianMutationU25CPromoterU6Available SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ITR-EcYtR-mCherry
Plasmid#217362PurposeExpresses E. coli tyrosine tRNA and a wild-type mCherry reporter; can be packaged into AAVDepositorInsertE. coli tyrosine tRNA
UseAAVExpressionMammalianPromoterU6Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only