We narrowed to 17,204 results for: puro

1. pLJM1 Flag Raptor HRas25

   Plasmid

   #26637

   Depositor

   David Sabatini

   Article

   Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

   Insert

   raptor-HRas25 (RPTOR Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

2. mLPx-shZMPSTE24

   Plasmid

   #69067

   Purpose

   encodes a shRNA against the protease ZMPSTE24

   Depositor

   Gerardo Ferbeyre

   Article

   Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

   Insert

   shRNA against ZMPSTE24 (ZMPSTE24 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

3. p6644 MSCV-P C-Flag HA 45E7-Kozak

   Plasmid

   #35135

   Depositor

   Peter Howley

   Article

   White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

   Insert

   HPV45 E7
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2012

   Availability

   Academic Institutions and Nonprofits only

4. p6640 MSCV-P C-FlagHA 16E7-Kozak

   Plasmid

   #35018

   Depositor

   Peter Howley

   Article

   White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

   Insert

   HPV16 E7 (E7 HPV 16)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV LTR

   Available since

   March 14, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pXPR_003 sgMSLN

   Plasmid

   #193588

   Purpose

   MSLN knockout

   Depositor

   William Hahn

   Article

   Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

   Insert

   sgMSLN (MSLN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. YSEHPTFTSQY_epitope

   Plasmid

   #165016

   Purpose

   Expression of CMV UL83 (363-373) fused to IL-2 signal sequence

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   CMV UL83 (363-373)
   

   Use

   Lentiviral

   Tags

   human IL2 signal sequence at N-term

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Flag pLJM1 RagD 77L

   Plasmid

   #19317

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   Ras-related GTP binding D (RRAGD Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S77L

   Promoter

   Available since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

8. px459 VRER

   Plasmid

   #101716

   Purpose

   sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E and T1337R)

   Promoter

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CAGhP14

   Plasmid

   #84362

   Purpose

   express FLAG-HA tagged human PRDM14

   Depositor

   Danny Reinberg

   Article

   Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

   Insert

   PRDM14 (PRDM14 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   silent mutations at coding silent 1068CtoT, 1629T…

   Promoter

   CAG

   Available since

   Feb. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pSMP-YY1_1

    Plasmid

    #36357

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    YY1 (YY1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

11. p667-UBC-CDC42BPB-V5-miniTurbo_IDG-K

    Plasmid

    #198008

    Purpose

    Gateway destination clone of CDC42BPB (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    CDC42BPB-V5-miniTurbo (CDC42BPB Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p6656 MSCV-P C-Flag HA 25E7-Kozak

    Plasmid

    #35149

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    HPV25 E7
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2012

    Availability

    Academic Institutions and Nonprofits only

13. P2A-NLS-tdtomato-EPG backbone

    Plasmid

    #215548

    Purpose

    An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFP

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p663-UBC-miniTurbo-V5-CSNK1G2_IDG-K

    Plasmid

    #214459

    Purpose

    Gateway destination clone of CSNK1G2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CSNK1G2 (CSNK1G2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p663-UBC-miniTurbo-V5-CAMKK2_IDG-K

    Plasmid

    #170577

    Purpose

    Gateway destination clone of CAMKK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CAMKK2 (CAMKK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p663-UBC-miniTurbo-V5-CDK17_IDG-K

    Plasmid

    #158174

    Purpose

    Gateway destination clone of CDK17 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDK17 (CDK17 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p6652 MSCV-P C-Flag HA 8E7-Kozak

    Plasmid

    #35145

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    HPV8 E7
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2012

    Availability

    Academic Institutions and Nonprofits only

18. p6653 MSCV-P C-Flag HA 92E7-Kozak

    Plasmid

    #35146

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    HPV92 E7
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2012

    Availability

    Academic Institutions and Nonprofits only

19. p6654 MSCV-P C-Flag HA 98E7-Kozak

    Plasmid

    #35148

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    HPV98 E7
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2012

    Availability

    Academic Institutions and Nonprofits only

20. p6651 MSCV-P C-Flag HA 76E7-Kozak

    Plasmid

    #35144

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    HPV76 E7
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2012

    Availability

    Academic Institutions and Nonprofits only

21. p6645 MSCV-P C-Flag HA 57E7-Kozak

    Plasmid

    #35136

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    HPV57 E7
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2012

    Availability

    Academic Institutions and Nonprofits only

22. pLKO mouse ULK2 shRNA 93

    Plasmid

    #27635

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    mouse ULK2 shRNA 93
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2011

    Availability

    Academic Institutions and Nonprofits only

23. p667-UBC-BRSK2-V5-TurboID_IDG-K

    Plasmid

    #135253

    Purpose

    Gateway destination clone of BRSK2 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    BRSK2-V5-TurboID (BRSK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLPCX roGFP2-Prx2(C172A)

    Plasmid

    #158228

    Purpose

    Genetically encoded fluorescent H2O2 sensor roGFP2-Prx2(C172A) for cytosolic expression in mammalian cells (for stable cell line generation)

    Depositor

    Tobias Dick

    Article

    Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

    Insert

    roGFP2-Prx2(C172A)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p667-UBC-CDK12-V5-miniTurbo_IDG-K

    Plasmid

    #135243

    Purpose

    Gateway destination clone of CDK12 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    CDK12-V5-miniTurbo (CDK12 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSMP-Suv39H1_1

    Plasmid

    #36342

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    Suv39H1 (SUV39H1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

27. LPCX Smad2

    Plasmid

    #12636

    Depositor

    Rik Derynck

    Article

    Choy et al J Cell Biol. 2000 May 1. 149(3):667-82.

    Insert

    Smad2 (SMAD2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p663-UBC-TurboID-V5-BRSK2_IDG-K

    Plasmid

    #135272

    Purpose

    Gateway destination clone of BRSK2 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    TurboID-V5-BRSK2 (BRSK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLKO.1P shTFRC

    Plasmid

    #169882

    Purpose

    Suppress TRFC

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    shRNA against TFRC
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMN-018

    Plasmid

    #195422

    Purpose

    AAVS1 Integratable mCherry Reporter for synZiFTR ZF10

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    4X ZF10 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p663-UBC-miniTurbo-V5-DYRK1B_IDG-K

    Plasmid

    #158183

    Purpose

    Gateway destination clone of DYRK1B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-DYRK1B (DYRK1B Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PX459-APP-KO

    Plasmid

    #176487

    Purpose

    To knockout APP gene

    Depositor

    Wade Harper

    Article

    Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

    Insert

    gRNA sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSMP-YY1_2

    Plasmid

    #36358

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    YY1 (YY1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

34. pMRX-IP-GFP-LC3-mRuby3

    Plasmid

    #184898

    Purpose

    Stable expression of GFP-LC3-mRuby3 in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Okawa et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    LC3B
    

    Use

    Tags

    EGFP and mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLenti-LbCpf1-U6_FlipArray

    Plasmid

    #109349

    Purpose

    Lentiviral vector to express Cpf1 from EFS promoter, as well as a Cpf1 FlipArray from U6 promoter.

    Depositor

    Sidi Chen

    Article

    Chow et al Nat Commun. 2018 May 15;9(1):1903. doi:

    Insert

    U6-FlipArray
    

    Use

    CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. p6868 MSCV-IP N-V5 Zer1

    Plasmid

    #35100

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    Zer1 (ZER1 Human)
    

    Use

    Retroviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    March 9, 2012

    Availability

    Academic Institutions and Nonprofits only

37. pX459-HypaCas9-AAVS1_sgRNA

    Plasmid

    #183891

    Purpose

    pX459V2.0-HypaCas9 plasmid with sgRNA targeting the AAVS1 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    AAVS1 sgRNA spacer (AAVS1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LbCas12a

    Plasmid

    #188493

    Purpose

    Expresses FLAG tagged LbCas12a

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. CRISPR_TRAC

    Plasmid

    #164993

    Purpose

    Expression of gRNA targeting TCRalpha constant locus

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against TRAC
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMXs-IP-EGFP-ULK2

    Plasmid

    #38201

    Depositor

    Noboru Mizushima

    Article

    Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

    Insert

    Unc-51 like kinase 2 (Ulk2 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only