We narrowed to 19,449 results for: tec
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Plasmid#119769PurposeConversion of C to T monocotyledons protoplastsDepositorInsertGam-APOBEC3A-nCas9-NLS-2XUGI-NLS
TagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available SinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
CP1249-CBEmax
Plasmid#119804PurposeC-to-T base editorDepositorInsertrApobec1-nSpCas9 (CP1249) -UGI-UGI
ExpressionMammalianAvailable SinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIS002
Plasmid#194013PurposeThis plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.DepositorInsertC16 Ex3 XTR f1
ExpressionBacterialAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
CP1028-ABEmax
Plasmid#119807PurposeA-to-G base editorDepositorInsertecTadA-ecTadA*(7.10)-nSpCas9 (CP1028)
ExpressionMammalianAvailable SinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAJAK-71 (pESC-NatMXsyn-USER)
Plasmid#78232PurposeEmpty vector, for the insertion of gRNA expression cassettes into the vector.DepositorTypeEmpty backboneExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
CL7-LbCas12aD156R
Plasmid#164659PurposeBacterial expression of mutated LbCas12aD156R using CL7/Im7DepositorInsertLbCas12a-D156R
TagsThioredoxin, CL7, SUMOExpressionBacterialMutationchanged Aspartic Acid 156 to ArgininePromoterT7Available SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAWH
Plasmid#133894PurposeExpression of Gal4AD-Prey hybrid protein. Homology regions for recombination with pBKWH, pBSWHhc or pMS22HDepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBKWH
Plasmid#133898PurposeExpression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWHDepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUPD2_miniDFR (GB2566)
Plasmid#160595PurposeMinimal promoter of slDFR gene containing 62bp upstream the transcription start site and the 5'UTR regionDepositorInsertminiDFR
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pShoCAST-sgRNA_entry (BO1)
Plasmid#181786PurposeExpresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
ExpressionBacterialPromoterLac and J23119Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Gfi1 NGFR
Plasmid#44630DepositorAvailable SinceApril 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMT462
Plasmid#139393PurposeXOR circuit (A000 and P000 design) with aTC and Bile Acid as inputs, AmpR, ErmRDepositorInsertXOR circuit (A000 and P000 design) with aTC and Bile Acid as inputs
UseSynthetic BiologyPromoteraTC and BA inducible promotersAvailable SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
NLS-GI-VP64-IRES-NLS-GAL4DBD-M13-SmBiT-FKF1
Plasmid#183400PurposeDual expression of GI-VP64 and GAL4DBD-M13-SmBiT-FKF1 in the nucleusDepositorInsertGI-VP64-IRES-GAL4DBD-M13-SmBiT-FKF1
ExpressionMammalianPromoterCMVAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFLIPglu-2uDelta13
Plasmid#12990DepositorInsertFLIPglu F16F D152A Delta13
UsePlant nanosensorTagsECFP and EYFPExpressionPlantMutationD152A mutation in binding proteinAvailable SinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
CP1012-CBEmax
Plasmid#119801PurposeC-to-T base editorDepositorInsertrApobec1-nSpCas9 (CP1012) -UGI-UGI
ExpressionMammalianAvailable SinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTS2386-Tier1-PhCMV-GCaMP6s
Plasmid#169519PurposeTier-1 vector encoding PhCMV-driven calcium sensor GCaMP6s (PhCMV-GCaMP6s-pA).DepositorInsertPCMV-driven green fluorescent genetically encoded calcium indicator GCaMP6s
ExpressionMammalianPromoterPhCMVAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMET7-GAG-HRAS
Plasmid#80604PurposeMammalian expression vector that expresses the HIV-1 GAG protein with HRAS fused to itDepositorInsertHIV-1 p55 GAG
ExpressionMammalianPromoterSRalphaAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
1137=Hsp70Bb-Cas9[dsRed]
Plasmid#149424PurposeThe piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)DepositorInsertCas9
UseCRISPRTagseGFPExpressionInsectPromoterDmel Hsp70BbAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC1750
Plasmid#127984PurposeAMA1 plasmid with Aspergillus optimized Cpf1, ble selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -