We narrowed to 28,416 results for: REP

1. pcDNA3 Mito(OM)-deltaCKAR

   Plasmid

   #222431

   Purpose

   FRET-based reporter for monitoring deltaPKC activity at the mitochondrial outer membrane in cells.

   Depositor

   Alexandra Newton

   Article

   Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

   Insert

   Mitochondrial outer membrane delta-selective C kinase activity reporter
   

   Use

   Tags

   CFP and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJB65

   Plasmid

   #226319

   Purpose

   E. coli protein expression for protein purification

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   6xHis- (VTG to AAA MR1-6)-strep
   

   Use

   Tags

   6xHis and Strep

   Expression

   Bacterial

   Mutation

   N-terminal domain deleted, C-terminal domain dele…

   Promoter

   T7

   Available since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRAR092-L31

   Plasmid

   #185045

   Purpose

   J23108(spel), pHP14 stability hairpin, strong RBS, L31 coding region, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRAR112

   Plasmid

   #185048

   Purpose

   J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region K2A mutation, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31p
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   K2A amino acid mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRAR113

   Plasmid

   #185049

   Purpose

   J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region H6A mutation, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31p
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   H6A amino acid mutaitons

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRAR114

   Plasmid

   #185050

   Purpose

   J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region E8A mutation, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31p
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E8A amino acid mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRAR120

   Plasmid

   #185041

   Purpose

   J23108 promoter, l31 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31-sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Only first 90 nt of rpmE coding present

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRAR089-L36p

   Plasmid

   #185042

   Purpose

   J23108(spel), pHP14 stability hairpin, strong RBS, L36p coding region, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L36p
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRAR090-L36

   Plasmid

   #185043

   Purpose

   J23108(spel), pHP14 stability hairpin, strong RBS, L36 coding region, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L36
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRAR091-L31p

    Plasmid

    #185044

    Purpose

    J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31p
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTcI38-PRM-wasabi

    Plasmid

    #195554

    Purpose

    Temperature-dependent expression of mWasabi reporter with transcription activation by TcI38

    Depositor

    Mikhail Shapiro

    Article

    Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

    Inserts

    mWasabi
    TcI38
    

    Use

    Tags

    6x His and AANDENYADAS degradation tag

    Expression

    Bacterial

    Mutation

    A67T, M1V, L65S, K68R, F115L, D126G, D188G

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pS44i8GH-2

    Plasmid

    #207526

    Purpose

    Conditionally-replicating in Pseudomonas vector, high-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; SmR/SpR

    Depositor

    Pablo Ivan Nikel

    Article

    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

    Insert

    oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP
    

    Use

    CRISPR; Pseudomonas vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYUBcLIC-GFP

    Plasmid

    #183509

    Purpose

    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in mycobacteria.

    Depositor

    Martin Högbom

    Article

    Grave et al Protein Expr Purif. 2022 Oct;198:106132.

    Type

    Empty backbone

    Use

    Tags

    10x His-tag and folding reporter GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. LLP729_L2_pV01_0I_FRT-OCS-rLUC_TCTP-fLUC

    Plasmid

    #192372

    Purpose

    To test for the repressive potential of the OCS with the FRT sites around it. Then used as a control to represent the off state of many cicuits.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmx-puro-Mef2c-i

    Plasmid

    #172398

    Purpose

    Retroviral expression vector for murine direct cardiac reprogramming

    Depositor

    Li Qian

    Article

    Wang et al Cells. 2020 Jan 22;9(2). pii: cells90202

    Insert

    Mef2c-i (Mef2c Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-TAB-mOvol1

    Plasmid

    #120360

    Purpose

    piggyBac transposon for dox-inducible expression of Ovol1 (with Bgeo; ie. LacZ / NeoR fusion).

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    Ovol1 (Ovol1 Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on and CBh

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCbh-SpyCas9-T2A-puro-pA_U6-sgPolb (pE047)

    Plasmid

    #149350

    Purpose

    Mammalian expression vector for SpyCas9, puromycin resistance and human Polb guide RNA

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    SpyCas9
    

    Use

    CRISPR

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYZ_NAND4_L17_S24_S11_S19_S13

    Plasmid

    #132732

    Purpose

    T7 RNAP-driven expression of a 4-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 24, 11, 19, and 13

    Depositor

    Alexander Green, Peng Yin

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_NAND4_L17_S24_S11_S19_S13
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. BII-C3H2B

    Plasmid

    #133387

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYZ_NAND3_L11_S11_S13_S19

    Plasmid

    #132731

    Purpose

    T7 RNAP-driven expression of a 3-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 11, 13, and 19

    Depositor

    Alexander Green, Peng Yin

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_NAND3_L11_S11_S13_S19
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGAL4DBD-SuUR f.l

    Plasmid

    #112995

    Purpose

    P element vector for transposon insertion of full-length Drosophila SuUR into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Sher et al Genome Res. 2012 Jan;22(1):64-75. doi: 1

    Insert

    SuUR (SuUR Fly)
    

    Use

    P insertion vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pX459V2.0+N497A+Q695A+Q926A

    Plasmid

    #108300

    Purpose

    pX459 V2.0 (Plasmid #62988) with the N497A, Q695A, and Q926A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET24a_UDP_Xase_E162

    Plasmid

    #73160

    Purpose

    Expresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669 mutant E162A

    Depositor

    Sandra Gabelli

    Article

    Duong-Ly et al PLoS One. 2013 May 15;8(5):e64241. doi:

    Insert

    pET24a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    change glutamate 162 to alanine

    Promoter

    Available since

    March 22, 2016

    Availability

    Academic Institutions and Nonprofits only

24. pSpCas9_T2A_mCherry sgAAVS1

    Plasmid

    #192480

    Purpose

    All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as control

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Use

    Tags

    T2A mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSpCas9_T2A_mCherry sg1-sg2

    Plasmid

    #192479

    Purpose

    All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Use

    Tags

    T2A mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. HIV-1 LTR-gfp

    Plasmid

    #115809

    Purpose

    HIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vpr

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    gfp and ngfr
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    HIV1 LTR

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSpCas9_T2A_iRFP670 sgAAVS1

    Plasmid

    #192477

    Purpose

    All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a control

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Use

    Tags

    T2A iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSpCas9_T2A_iRFP670 sgBFP

    Plasmid

    #192478

    Purpose

    All-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Use

    Tags

    T2A iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSpCas9_T2A_mCherry sgBFP

    Plasmid

    #192929

    Purpose

    All-in-one plasmid for cutting BFP region in DSB Spectrum V2

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Use

    Tags

    T2A mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 7xSMAD_RE::FLuc

    Plasmid

    #124531

    Purpose

    SMAD2 FLuc luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    SMAD FLuc reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge