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Showing: 10041 - 10080 of 17519 results

  3. pCas9GG

    Plasmid
    #131009
    Purpose
    Backbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of SpCas9
    promoter PJ23119
    mRFP expression cassette
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Bba_R0040 TetR
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    131009-plasmid-map-sequence-id-257082

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  15. pcDNA3-ABKAR-Kras

    Plasmid
    #84637
    Purpose
    Unimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targeted
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    Cerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras
    Tags
    6xHis, C-terminal targeting sequence from Kras, C…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    84637-plasmid-map-sequence-id-176126

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  18. pChlr-1SR

    Plasmid
    #52142
    Purpose
    Encodes module 1 with amino acids 12S-16R for recognition of nt 8C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 25-60 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 16Q to 16R in Module 1
    Available Since
    May 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pChlr-1NQ

    Plasmid
    #52141
    Purpose
    Encodes module 1 with amino acids 12N-16Q for recognition of nt 8U
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 25-60 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12S to 12N in Module 1
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pChlr-2CQ

    Plasmid
    #52143
    Purpose
    Encodes module 2 with amino acids 12C-16Q for recognition of nt 7A
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 61-96 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12C in Module 2
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  21. pChlr-2SR

    Plasmid
    #52146
    Purpose
    Encodes module 2 with amino acids 12S-16R for recognition of nt 7C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 61-96 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16R in Module 2
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  22. pChlr-3NQ

    Plasmid
    #52149
    Purpose
    Encodes module 3 with amino acids 12N-16Q for recognition of nt 6U
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 97-132 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12C to 12N in Module 3
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  23. pChlr-3SR

    Plasmid
    #52150
    Purpose
    Encodes module 3 with amino acids 12S-16R for recognition of nt 6C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 97-132 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12C to 12S and 16Q to 16R in Module 3
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  24. pChlr-4CQ

    Plasmid
    #52151
    Purpose
    Encodes module 4 with amino acids 12C-16Q for recognition of nt 5A
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12C in Module 4
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  25. pChlr-4SYE

    Plasmid
    #52156
    Purpose
    Encodes module 4 with amino acids 12S-13Y-16E for recognition of nt 5G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    D43951 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S, 13H to 13Y, and 16Q to 16E in…
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  26. pChlr-4NYQ

    Plasmid
    #52157
    Purpose
    Encodes module 4 with amino acids 12N-13Y-16Q for recognition of nt 5U
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 13H to 13Y in Module 4
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  27. pChlr-5NQ

    Plasmid
    #52161
    Purpose
    Encodes module 5 with amino acids 12N-16Q for recognition of nt 4U
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 169-204 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12C to 12N in Module 5
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  28. pChlr-6CQ

    Plasmid
    #52163
    Purpose
    Encodes module 6 with amino acids 12C-16Q for recognition of nt 3A
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 205-240 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12C in Module 6
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  37. pTNos:BASTA:PNos (GB0023)

    Plasmid
    #68211
    Purpose
    Provides the bar gene for resistance to basta fused to the A. tumefaciens Nopaline synthase promoter and terminator. Cloned as a level 0 GB part ready to be transferred to a GB level1 plant expression vector.
    Depositor
    Diego Orzaez
    Article
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    bar gene
    Use
    Unspecified
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    PNos
    Available Since
    Oct. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    68211-plasmid-map-sequence-id-119063

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Showing: 10041 - 10080 of 17519 results