159,332 results
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AAVS1-iPE-Plus
Plasmid#214062Purposeinducible PE-Plus system for controllable prime editing; this plasmid is used to insert PE-Plus prime editor (PEmax-P2A-hP53DD-IRES-MLH1dn) in one allele of AAVS1 locusDepositorInsertPE-Plus
UseTagsExpressionMammalianMutationPromoterTRE-tightAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMetYC-Dest
Plasmid#105081PurposeYeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.DepositorTypeEmpty backboneUseTagsCub-PLVExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
c-Myc-5-HT2A
Plasmid#67944PurposeN-terminally c-myc tagged human 5-hydroxytryptamine receptor 2ADepositorInsertHTR2A (HTR2A Human)
UseTagsc-MycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
mPA-GFP-Actin-C-18
Plasmid#57121PurposeLocalization: Actin, Excitation: 400 / 504, Emission: 515 / 517DepositorInsertActin (ACTB Human)
UseTagsmPA-GFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
LEPZ
Plasmid#111161PurposemiR-E (miR-30 variant)-based RNAi with ZsGreen reporterDepositorInsertmiR-E (miR-30 variant)
UseRetroviralTagsExpressionMutationWTPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2-Vimentin-7
Plasmid#57485PurposeLocalization: Intermediate Filaments, Excitation: 507 / 572, Emission: 516 / 580DepositorInsertVimentin (VIM Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
T7-CMVtrans-FFLuc-polyA
Plasmid#101156PurposeTemplate for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterT7 promoterAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX_TRC311 ETV4-S
Plasmid#74983PurposeConstitutive ETV4 ORF, blasticidin selectionDepositorInsertETV4 (ETV4 )
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCold CL7-Cas9
Plasmid#124890Purposeobtaining full Cas9 RNPDepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
N-BLa 1.2
Plasmid#88999PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.DepositorInsertN-BLa 1.2 fusion protein
UseTagsFlagExpressionBacterialMutationPromoteraraBADAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
C-BLa 1.2
Plasmid#89000PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the C-terminal fragment of the split β-lactamase.DepositorInsertC-BLa 1.2 fusion protein
UseTagsFlagExpressionBacterialMutationPromoteraraBADAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro_MYRF
Plasmid#64843PurposeA lentiviral vector for the expression of mouse MYRF under doxycyline control.DepositorInsertMYRF (Myrf Mouse)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pR-mKate2
Plasmid#228610PurposeMammalian expression of mKate2, for MuSIC barcode constructionDepositorInsertmKate2
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
p31sheLL
Plasmid#37322DepositorInsertHPV31L1+L2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pC1-HyPerRed-mito
Plasmid#60247PurposeMitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.DepositorInsertHyPerRed-mito
UseTagsMitochondrial targeting signalExpressionMammalianMutationPromoterCMVAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEMB52/14-3-3_1_247
Plasmid#40541DepositorInsertYWHAG (YWHAG Human)
UseBaculovirusTags6xHisExpressionMutationWT; aa1-247PromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMSCV-IRES-DsRed FP (pMIDsRed II)
Plasmid#52110Purposea bicistronic IRES-DsRed containing retroviral vector, MCS is expanded from pMSCV-IRES-GFPDepositorTypeEmpty backboneUseRetroviralTagsIRES-DsRedExpressionMammalianMutationPromoterAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
mApple-N1
Plasmid#54567PurposeLocalization: N1 Cloning Vector, Excitation: 568, Emission: 592DepositorTypeEmpty backboneUseTagsmAppleExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
mPA-GFP-Calnexin-N-14
Plasmid#57122PurposeLocalization: Endoplasmic Reticulum, Excitation: 400 / 504, Emission: 515 / 517DepositorInsertCalnexin (CANX Human)
UseTagsmPA-GFPExpressionMammalianMutationI474N in CalnexinPromoterCMVAvailable sinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
TFORF0707
Plasmid#143889PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNCOR1 (NCOR1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)
Plasmid#209078PurposeNgn2 mediated cortical neuron induction, along with SNCA(3K) over-expressionDepositorUseTagssfGFPExpressionMammalianMutation3K mutant: E35K, E46K, E61KPromoterTRE4GAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_DA2m
Plasmid#140553PurposeExpresses the 2nd generation medium-affinity DA sensor GRAB_DA2m in neuronsDepositorHas ServiceAAV9InsertThe 2nd generation DA sensor GRAB_DA2m (medium affinity)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC18
Plasmid#50004PurposepUC cloning vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterlacAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAM5411
Plasmid#132663PurposeRSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistanceDepositorInsertsaadA
YFP
UseTagsExpressionBacterialMutationPromoterPaadA and PconIIAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c_ddCpf1_final
Plasmid#212040PurposeBacterial expression of the CRISPRi system for genome editing in E. coliDepositorInsertddCpf1
UseCRISPRTagsExpressionMutationPromoterL-arabinose-inducible promoter PBADAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-muOR-GFP11-T2A-GFP10-bArrestin1-T2A mCherry
Plasmid#113611PurposePPI reporterDepositorInsertmu opioid receptor
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ga13_2-mTq2
Plasmid#112933PurposeHuman Galpha13 tagged with mTurquoise, inserted at residue 128DepositorInsertGalpha13
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
mPA-GFP-Farnesyl-5
Plasmid#57133PurposeLocalization: Membrane, Excitation: 400 / 504, Emission: 515 / 517DepositorInsertFarnesyl (HRAS Human)
UseTagsmPA-GFPExpressionMammalianMutationencodes final 20 aa of NM_001130442.1PromoterCMVAvailable sinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENTR-LUC (w158-1)
Plasmid#17473PurposeGateway entry vector, Firefly LuciferaseDepositorInsertFirefly Luciferase
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7556 pHR (hU6-crCD46-EFS-PuroR-WPRE)
Plasmid#214880PurposeLentiviral vector encoding RfxCas13d targeting CD46 guideDepositorInserthU6-crCD46-EFS-PuroR-WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
A*02:02:01:01
Plasmid#203277PurposeOverexpress HLA alleleDepositorInsertA*02:02:01:01 (HLA-A Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_ETV4
Plasmid#167753PurposeExpresses ETV4 in mammalian cellsDepositorInsertETV4 (ETV4 Human)
UseTagsMACExpressionMammalianMutationno mutationsPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEntry_SARS-CoV-2-Nucleocapsid
Plasmid#168886PurposeGateway-compatible Entry vectorDepositorInsertSARS-CoV-2-Nucleocapsid (N )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTKDP-neo
Plasmid#71320PurposeDonor plasmid - kanamycin resistant donor cassetteDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
BirA pLEMO
Plasmid#119818PurposeExpress endogenous biotin ligase in E.coliDepositorInsertBiotin ligase
UseTagsExpressionBacterialMutationPromoterRhamnose promoterAvailable sinceApril 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgDrd2
Plasmid#159918PurposeMutagenesis of Drd2DepositorInsertDrd2 (Drd2 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-mArg1 promoter/enhancer -31/-3810
Plasmid#34571DepositorInsertArg1 promoter/enhancer -31/-3810 (Arg1 Mouse)
UseLuciferaseTagsFirefly LuciferaseExpressionMutationPromoterAvailable sinceJan. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-LIC
Plasmid#26099PurposeSGC Empty baculovirus expression/transfer vectorDepositorTypeEmpty backboneUseBaculovirus expressionTags6x His and Thrombin protease cleavage siteExpressionMutationPromoterpolyhedrinAvailable sinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-Sec16A
Plasmid#36155PurposeExpresses Sec16A as a GFP fusionDepositorInsertSec16A (SEC16A Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only
