164,826 results

1. pRS415-GPD

   Plasmid

   #183940

   Purpose

   Control vector that has a GPD promoter with no insert

   Depositor

   Michael Downey

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-p300

   Plasmid

   #23252

   Depositor

   Warner Greene

   Article

   Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

   Insert

   p300 (EP300 Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST-CMV 3xFLAG-LC3B-GFP

   Plasmid

   #123107

   Purpose

   Expresses 3xFLAG-LC3B-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Use

   Tags

   3xFLAG and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-Q23

   Plasmid

   #40261

   Depositor

   David Rubinsztein

   Article

   Narain et al J Med Genet. 1999 Oct;36(10):739-46.

   Insert

   HTT partial exon 1 Q23 (HTT Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   expanded poly-Q

   Promoter

   CMV

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHIV-Luciferase

   Plasmid

   #21375

   Purpose

   self-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and Luciferase

   Depositor

   Bryan Welm

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 19, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJL1-sfGFP

   Plasmid

   #102634

   Purpose

   In vitro expression of sfGFP from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

   Insert

   sfGFP
   

   Use

   Tags

   strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRX-IB-HaloTag7-mGFP-KDEL

   Plasmid

   #184904

   Purpose

   Stable expression of HaloTag7-mGFP-KDEL in mammalian cells to measure ER-phagy activity

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   KDEL
   

   Use

   Tags

   HaloTag7 and monomeric EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. 19ag-SOD2-3xHA-TALE(RNR2-Left)-MutH

   Plasmid

   #209294

   Purpose

   mitoBE expression vector, target mtRNR2 gene.

   Depositor

   Wensheng Wei

   Article

   Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

   Insert

   SOD2-3xHA-TALE(RNR2-Left)-MutH
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. RFP-LC3B

   Plasmid

   #200943

   Purpose

   RFP fused with LC3B for reporting LC3B lipidation

   Depositor

   Nir Hacohen

   Article

   Liu et al Science. 2023 Aug 4;381(6657):508-514. d

   Insert

   LC3B (MAP1LC3B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pC014 - LwCas13a-msfGFP

    Plasmid

    #91902

    Purpose

    Expresses active LwCas13a for mammalian RNA targeting

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LwCas13a
    

    Use

    CRISPR

    Tags

    NLS and msfGFP-NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-N1-IL6

    Plasmid

    #111933

    Purpose

    Expresses human interleukin-6 (IL6) fused to GFP in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

    Insert

    Interleukin 6 (IL6 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Codon optimized for expression in human cells

    Promoter

    CMV

    Available Since

    July 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-mTurquoise2-Caspase-1-mNeonGreen

    Plasmid

    #166784

    Purpose

    FRET analysis (pro caspase-1 cleavage) in living cells

    Depositor

    Hao Wu

    Article

    Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

    Insert

    mTurquoise2-caspase-1-mNeonGreen (CASP1 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen and mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. hsgk1

    Plasmid

    #83432

    Purpose

    full length human sgk1 with Flag tag

    Depositor

    Christie Thomas

    Article

    Raikwar et al Am J Physiol Renal Physiol. 2008 Nov;295

    Insert

    human Sgk1 (SGK1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYC-C

    Plasmid

    #207114

    Purpose

    HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

    Depositor

    Yifan Cheng

    Article

    Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. CRBN_ΔHBD

    Plasmid

    #232779

    Purpose

    Expresses CRBN_ΔHBD sequence in E. coli cells with cleavable MBP-His tag and deleted internal DDB1 binding domain

    Depositor

    Ivan Dikic

    Article

    Bailey et al Cell Chem Biol. 2024 Nov 20:S2451-9456(2

    Insert

    Cereblon (CRBN Human)
    

    Use

    Tags

    MBP-His6-TEV

    Expression

    Bacterial

    Mutation

    CRBN (residues 47 to 193 and 249 to 436) with GNG…

    Promoter

    T7

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. iF59 PB-TO-oNgn2-puro

    Plasmid

    #204734

    Purpose

    Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Optimized neurogenin-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDDGFP_LEU2D

    Plasmid

    #58352

    Purpose

    Used for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiae

    Depositor

    Simon Newstead

    Article

    Parker et al Protein Sci. 2014 Jun 20. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    GFP-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCSII-EF/mt-(n1)StayGold

    Plasmid

    #185823

    Purpose

    A soluble photostable fluorescent marker of the mitochondrial matrix. The StayGold gene has codon optimization for mammalian expression.

    Depositor

    Atsushi Miyawaki

    Article

    Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

    Insert

    mt-(n1)StayGold
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKOV

    Plasmid

    #25769

    Depositor

    George Church

    Article

    Link et al J Bacteriol. 1997 Oct . 179(20):6228-37.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-rab11 WT

    Plasmid

    #12674

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB11A (Homo sapiens) (RAB11A Human)
    

    Use

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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