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pYLCRISPR/Cas9P35S-H

Plasmid

#66189

Purpose

expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), hygro selection

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Use

Tags

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

cauliflower mosaic virus 35S promoter (P35S)

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3 Control Vector

Plasmid

#212937

Purpose

Control vector with constitutive firefly luciferase expression

Depositor

Debrya Groskreutz

Insert

luc+

Use

Luciferase

Tags

Expression

Mutation

WT

Promoter

SV40

Available since

Jan. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.3_KLF4

Plasmid

#26815

Depositor

Derrick Rossi

Article

Warren et al Cell Stem Cell. 2010 Sep 29. ():.

Insert

5'UTR-KLF4-3'UTR (KLF4 Human)

Use

Tags

Expression

Mammalian

Mutation

Starts from second ATG

Promoter

Available since

Nov. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-C2 SENP2

Plasmid

#13382

Depositor

Mary Dasso

Article

Hang et al J Biol Chem. 2002 May 31. 277(22):19961-

Insert

SENP2 (SENP2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-rsGreenF-E-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-Skylan62A-2A-YFP-SLBP

Plasmid

#205762

Purpose

Single-color FUCCI4 senor for transitions of all four cell cylce phases

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsGFE-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-SK62A-2A-YFP-SLBP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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ISceI-GR-RFP

Plasmid

#17654

Depositor

Tom Misteli

Article

Soutoglou et al Nat Cell Biol. 2007 Jun . 9(6):675-82.

Insert

I-SceI-GRLBD (SCEI, Nr3c1 Rat, Budding Yeast)

Use

Tags

mRFP

Expression

Mammalian

Mutation

C655G mutation in the glucocorticoid receptor (se…

Promoter

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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pSL-MS2-12X

Plasmid

#27119

Depositor

Robert Singer

Article

Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

Insert

MS2 binding sites (12)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 20, 2010

Availability

Academic Institutions and Nonprofits only

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GFP-P4M-SidMx2

Plasmid

#51472

Purpose

Biosensor for PtdIns4P

Depositor

Tamas Balla

Article

Hammond et al J Cell Biol. 2014 Apr 7.

Inserts

P4M-SidM(546-647)
P4M-SidM(546-647)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pXR004: CasRx pre-gRNA cloning backbone

Plasmid

#109054

Purpose

hU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs

Depositor

Patrick Hsu

Article

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-human Erbb2-IRES-GFP

Plasmid

#91888

Purpose

Retroviral expression of human Erbb2

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Erbb2 (ERBB2 Human)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Mutation

Promoter

MMLV gag

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3 BRE Luciferase

Plasmid

#45126

Depositor

Martine Roussel, Peter ten Dijke

Article

Korchynskyi et al J Biol Chem. 2002 Feb 15;277(7):4883-91.

Insert

Id1-BRE

Use

Luciferase; Bmp/smad transcriptional reporter

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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Active Wnt16-V5

Plasmid

#43825

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

Wnt16 (WNT16 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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mEGFP-pBAD

Plasmid

#54622

Purpose

Localization: Protein Expression Vector , Excitation: 488, Emission: 507

Depositor

Michael Davidson

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

6xHis and mEGFP

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV_BiCHATe27_dTomato_nlsdTomato

Plasmid

#213829

Purpose

AAV construct expressing dTomato driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_dTomato_nlsdTomato

Depositor

Gordon Fishell

Article

Furlanis et al bioRxiv 2024.07.17.603924

Has Service

AAV PHP.eB

Insert

dTomato_P2A_nls.dTomato

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAC150-KEIMA-REEP5

Plasmid

#201928

Purpose

PiggyBac Vector expressing KEIMA-REEP5

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

REEP5 (REEP5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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SpdCas9 VPR

Plasmid

#188509

Purpose

Expresses FLAG tagged SpdCas9 VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A/H840A

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX iLOV

Plasmid

#26587

Depositor

John Christie

Article

Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 1

Insert

iLOV

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-YFP-Turbo-L2

Plasmid

#127350

Purpose

gateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteins

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Use

Gateway entry vector

Tags

GS linker, V5, and YFP

Expression

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

no promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-SUMO-MarathonRT

Plasmid

#109029

Purpose

Group II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”

Depositor

Anna Pyle

Article

Zhao et al RNA. 2018 Feb;24(2):183-195. doi: 10.126

Insert

ERE_01490

Use

Tags

6xHis-SUMO tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti‐Cas9‐2A‐Blast

Plasmid

#73310

Purpose

Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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