We narrowed to 27,285 results for: Tat
-
Plasmid#46505DepositorInsertSocs2 (SOCS2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 28-162PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYPQ239-RVR
Plasmid#108863PurposeFnCpf1 Gateway entry plasmid with N623R, K629R and N633R mutationsDepositorInsertFnCpf1-RVR (FnCpf1 with N623R, K629R and N633R mutations)
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationN623R, K629R and N633R, Cpf1 is rice codon optimi…PromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF-G466V
Plasmid#131722PurposeMammalian Expression of Flag tagged BRAFDepositorInsertBRAF (BRAF Human)
UseTags3xFLAGExpressionMammalianMutationG466VPromoterCMVAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
LADL Bridge + Promoter Only Target
Plasmid#127669PurposeEncodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promotersDepositorInsertCRY2-HA-2A-mCherry and gRNAs 115 and 117
UseCRISPRTagsHAExpressionMammalianMutationPromoterEF1a and hU6Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJJR50
Plasmid#75026PurposeU6 promoter driven flipped + extended sgRNA expression vectorDepositorInsertguide RNA, flipped and extended version
UseCRISPRTagsExpressionMutationPromoterR07E5.16 (U6)Available sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-GRM3 (E767K)
Plasmid#31801DepositorInsertGRM3 (GRM3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationChanged glutamic acid 767 to lysinePromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-GRM3 (G561E)
Plasmid#31799DepositorInsertGRM3 (GRM3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationChanged glycine 561 to glutamic acidPromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mkgDUX4mal*mqg*
Plasmid#21174DepositorInsertDUX4 (DUX4 Human)
UseTagsExpressionBacterial and MammalianMutationFirst point mutation at nucleotide 5114 (numberin…PromoterAvailable sinceAug. 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-S400F_RASA2-EF1-Puro
Plasmid#114098PurposeExpress mutant RASA2 in mamalian cellsDepositorInsertRASA2 (RASA2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationS400FPromoterCMVAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II no force control (FL-based)
Plasmid#118724PurposeThe no force control for the FL-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.DepositorInserthuman Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry] (DSP Human)
UseRetroviralTagsYPet(short)-FL-mCherryExpressionMammalianMutationtruncation after aa1353PromoterCMVAvailable sinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hE2F7mg_2ntmut
Plasmid#73074Purposehuman E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding siteDepositorInsertE2F7 (E2F7 Human)
UseTagsExpressionMammalianMutationpartial gene, exons 11,12,13 spaced by introns, 2…PromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUW TetO NET1A-V5 S46A
Plasmid#69829PurposeExpresses NET1A-V5 in mammalian cells in a doxycycline inducible mannerDepositorInsertNeuroepithelial cell-transforming gene 1 protein A (NET1 Human)
UseLentiviralTagsv5ExpressionMammalianMutationS46APromoterminimal CMV promoter with tetracycline operatorAvailable sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONOR-SNCAe3-WT
Plasmid#85847PurposeDonor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP and dTomatoDepositorInsertSNCA exon 3 homology arms (SNCA Human)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Venus-TmiR-G12
Plasmid#25740PurposeLentiviral vector with Tet-based inducible expression of mouse G alpha 12 miR30-based shRNA, constitutive Venus fluorescent protein coexpression.DepositorInsertG alpha 12 miR-shRNA (Gna12 Mouse)
UseLentiviral and RNAiTagsVenusExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
CMV-10-3xFLAG-LGP2-K651E
Plasmid#58685PurposeExpresses LGP2 with a mutation in the CTD (K651E) in mammalian cellsDepositorInsertLGP2-K651E (DHX58 Human)
UseTags3x FLAGExpressionMammalianMutationmutation to CTD, K651EPromoterCMVAvailable sinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZS1[FruR-T,TreR-T]
Plasmid#60772PurposeContains PIq driving expression FruR-T, and PIq driving expression of TreR-T.DepositorInsertsFruR-T
TreR-T
UseSynthetic BiologyTagsExpressionBacterialMutationLacI/GalR repressor chimera. TAN DBD with FruR LB…PromoterAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-NSF/S647A
Plasmid#74938PurposeMammalian expression of human NSF mutant S647A (mutation in the D2 domain of the protein)DepositorInsertNSF (NSF Human)
UseTagsFLAGExpressionMammalianMutationS647A (mutation in the D2 domain of the protein)PromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-CHD5-WT-331-672aa
Plasmid#68870PurposeMammalian expression of CHD5 histone binding domain (331-672aa)DepositorInsertCHD5 (CHD5 Human)
UseTagsFLAGExpressionMammalianMutation331-672aaPromoterCMVAvailable sinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRAF-WT-IRES-mCherry
Plasmid#221027PurposeFluorescent reporter for expressing a segment of wild type BRAFDepositorInsertBRAF wildtype (BRAF Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR-B_sgRNA_DDX59 (pAVA3317)
Plasmid#239299PurposeLentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting DDX59DepositorInsertU6-driven sgRNA targeting DDX59 (DDX59 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-DIO-ExRai-AKAR2-T/A
Plasmid#171847PurposeCre-dependent, synapsin promoter-driven expression of the negative control phosphomutant ExRai-AKAR2 T/A PKA biosensor in neurons.DepositorInsertExRai-AKAR2 T/A
UseAAVTagsExpressionMammalianMutationT6A phospho-deficient mutationPromoterhSyn1Available sinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUAS-spatzle-HA
Plasmid#240235PurposeExpression of spatzle-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.DepositorInsertspatzle (spz Fly)
UseTagsHA-spGFP11ExpressionInsectMutationPromoterUASAvailable sinceJuly 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA810 - pBA904 Puro-T2A-GFP HES7 g2 CRISPRa guide (pRCA360 backbone) 674
Plasmid#238177PurposeLentiviral CRISPR guide vector expressing a HES7 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct CaptureDepositorInsertHES7 sgRNA CRISPRa (HES7 Human, Synthetic)
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR2b-ITGB1(WT)
Plasmid#215450PurposeGateway entry vector with integrin beta1 wild-type (WT)DepositorInsertITGB1 (ITGB1 Human)
UseGateway entry vectorTagsExpressionMutationSilent mutations added to disrupt shRNA binding a…PromoternoneAvailable sinceMay 29, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
CMV_R-PTEN_4A
Plasmid#227435PurposeExpression of the R-PTEN sensor with a 4A mutationDepositorInsertR-PTEN 4A (Pten Rat)
UseTagsmCyRFP2, mMaroonExpressionMammalianMutationPromoterCMVAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CB-Cdkn1a
Plasmid#228914PurposeExpression CDKN1A in mammalian cellsDepositorInsertCdkn1a (Cdkn1a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD allW
Plasmid#234609PurposeBacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.DepositorInserthnRNPA1_LCD_allW (HNRNPA1 Human)
UseTags6xHis-TEVExpressionBacterialMutationC-terminal domain (186-320) with all aromatic ami…PromoterT7Available sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
HR700_HA2TP53_Ex5
Plasmid#228860PurposeBackbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 5DepositorTypeEmpty backboneUseCRISPR and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
HR700_HA2TP53_Ex6
Plasmid#228861PurposeBackbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 6DepositorTypeEmpty backboneUseCRISPR and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
HR700_HA2TP53_Ex7
Plasmid#228862PurposeBackbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 7DepositorTypeEmpty backboneUseCRISPR and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
HR700_HA2TP53_Ex8
Plasmid#228863PurposeBackbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 8DepositorTypeEmpty backboneUseCRISPR and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAGIG-CDKN1A
Plasmid#228912PurposeExpression CDKN1A in mammalian cellsDepositorInsertCdkn1a (Cdkn1a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGIG-shCdkn1a(#4)
Plasmid#228913PurposeKnockdown of Cdkn1a in mammalian cellsDepositorInsertshCdkn1a (Cdkn1a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRetroQ-GTSE1-acGFP-S91, 262, 454, 724D
Plasmid#224742PurposeRetroviral vector for delivery and expression of acGFP1-tagged GTSE1 protein with S91, 262, 454, 724D mutationDepositorInsertGTSE1 (GTSE1 Human)
UseRetroviralTagsacGFPExpressionMutationSerine 91, 262, 454, 724 Aspartate; Valine 53 Iso…PromoterCMVAvailable sinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MARK3-GK-CR
Plasmid#229424PurposeLentiviral or overpexression of cDNADepositorInsertMARK3-GK-CR (MARK3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationGatekeeper mutation M132G; silent mutation CRISPR…PromoterEFSAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MARK2-GK-CR
Plasmid#229423PurposeLentiviral or overpexression of cDNADepositorInsertMARK2-GK-CR (MARK2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationGatekeeper mutation M129G; silent mutation CRISPR…PromoterEFSAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EF1aL-E690Kmut_ABCA3-GFP
Plasmid#188549PurposeLentiviral vector allowing for EF1aL-driven constitutive expression of E690K mutant human ABCA3:GFP fusion proteinDepositorInsertABCA3:GFP (ABCA3 Human)
UseLentiviralTagsGFPExpressionMutationamino acid E690->K690 (ABCA3 gene)PromoterEF1aLAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EF1aL-W308Rmut_ABCA3-GFP
Plasmid#188550PurposeLentiviral vector allowing for EF1aL-driven constitutive expression of W308R mutant human ABCA3:GFP fusion proteinDepositorInsertABCA3 (ABCA3 Human)
UseLentiviralTagsGFPExpressionMutationamino acid W308->R308 (ABCA3 gene)PromoterEF1aLAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W3-W4-HECT_C874only
Plasmid#187711PurposeExpress his-tagged NEDD4L W3-W4-HECT_C874only in bacterial cellsDepositorInsertNEDD4L residues 492-975 with mutations (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-491; mutations C702A, C776…PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcs2-cDELE1(142-515)-sumo
Plasmid#226107PurposecDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) fused to sumo used for in-vitro translation by wheat germ extract in in-vitro ubiquitylation assaysDepositorInsertDELE1 (DELE1 Human)
UseTagsSumoExpressionMammalianMutationencodes only residues 142-515 of DELE1 (represent…PromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only